High-Level Expression of Ice Nuclei in Erwinia herbicola Is Induced by Phosphate Starvation and Low Temperature

1998 ◽  
Vol 36 (6) ◽  
pp. 370-376 ◽  
Author(s):  
Amy L. Fall ◽  
Ray Fall
Keyword(s):  
Low Temperature ◽  
Phosphate Starvation ◽  
High Level Expression ◽  
Erwinia Herbicola ◽  
Ice Nuclei ◽  
High Level

scholarly journals SNP in DFR1 Coding Sequence Is Tightly Associated with Anthocyanin Accumulation in Cabbage (B. oleracea var. capitata f. alba) at Low Temperature

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 602 ◽  
Author(s):  
Hayoung Song ◽  
Jong-In Park ◽  
Byung-Ho Hwang ◽  
Hankuil Yi ◽  
HyeRan Kim ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Amino Acid ◽  
Low Temperature ◽  
Anthocyanin Accumulation ◽  
Nucleotide Polymorphism ◽  
High Level Expression ◽  
Single Nucleotide ◽  
Coding Sequence ◽  
Low Level ◽  
High Level

Keeping green leaf color at the time of harvest is one of the important traits for breeding of Brassica oleracea var. capitata f. alba, and this trait is related to low anthocyanin contents. To understand the differential accumulation of anthocyanins in cabbage, we selected high anthocyanin accumulators (HAAs) and low anthocyanin accumulator (LAAs) of cabbages and examined the anthocyanin content and the expression of anthocyanin biosynthesis-related genes. Among many genes investigated, BoDFR1 was found to be closely related to anthocyanin accumulation, even under low temperature (LT) conditions. BoDFR1 sequence analyses between HAAs and LAAs revealed that there is a single nucleotide polymorphism (SNP) (1118T/A) in the coding sequence, which substitutes one amino acid from Leu261 to His261; we named BoDFR1 with His261 substitution as BoDFR1v. This amino acid substitution did not affect dihydroflavonol 4-reductase (DFR) activity and substrate specificity, but the polymorphism showed tight association to the BoDFR1 expression, i.e., high level expression of BoDFR1 and low level expression of BoDFR1v under LT conditions. The high levels of BoDFR1 expression were due to the high levels of BoMYB114 and BobHLH expressions combined with low level expression of BoMYBL2, a repressor MYB. On the other hand, low levels of BoDFR1v expression seemed to be related to very low level expressions of BoMYB114 and BobHLH combined with a high level expression of BoMYBL2. It seems that different expression levels of these regulatory genes for MBW (MYB-bHLH-WD40) complex between HAAs and LAAs regulate BoDFR expression and anthocyanin accumulation. Using a single nucleotide polymorphism (SNP) between BoDFR1 and BoDFR1v, molecular markers for PCR and high resolution melt analyses were developed and validated to distinguish between HAAs and LAAs. Combined use of the BoDFR1 SNP marker with other stress markers, such as a cold tolerant marker, will greatly improve cabbage breeding.

High-Level Expression of Human β-Defensin-2 Gene with Rare Codons in E. coli Cell-Free System

2006 ◽  
Vol 13 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Haiqin Chen ◽  
Zhinan Xu ◽  
Naizheng Xu ◽  
Peilin Cen
Keyword(s):  
Coli Cell ◽  
High Level Expression ◽  
Free System ◽  
Cell Free System ◽  
E Coli ◽  
Rare Codons ◽  
High Level

scholarly journals An opportunistic promoter sharing regulatory sequences with either a muscle-specific or a ubiquitous promoter in the human aldolase A gene.

1993 ◽  
Vol 13 (1) ◽  
pp. 9-17 ◽  
Author(s):  
J P Concordet ◽  
M Salminen ◽  
J Demignon ◽  
C Moch ◽  
P Maire ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Integration Site ◽  
Regulatory Elements ◽  
Regulatory Sequences ◽  
Beta Globin ◽  
Fast Skeletal Muscle ◽  
High Level Expression ◽  
Adult Skeletal Muscle ◽  
High Level ◽  
Aldolase A

The human aldolase A gene is transcribed from three different promoters, pN, pM, and pH, all of which are clustered within a small 1.6-kbp DNA domain. pM, which is highly specific to adult skeletal muscle, lies in between pN and pH, which are ubiquitous but particularly active in heart and skeletal muscle. A ubiquitous enhancer, located just upstream of pH start sites, is necessary for the activity of both pH and pN in transient transfection assays. Using transgenic mice, we studied the sequence controlling the muscle-specific promoter pM and the relations between the three promoters and the ubiquitous enhancer. A 4.3-kbp fragment containing the three promoters and the ubiquitous enhancer showed an expression pattern consistent with that known in humans. In addition, while pH was active in both fast and slow skeletal muscles, pM was active only in fast muscle. pM activity was unaltered by the deletion of a 1.8-kbp region containing the ubiquitous enhancer and the pH promoter, whereas pN remained active only in fast skeletal muscle. These findings suggest that in fast skeletal muscle, a tissue-specific enhancer was acting on both pN and pM, whereas in other tissues, the ubiquitous enhancer was necessary for pN activity. Finally, a 2.6-kbp region containing the ubiquitous enhancer and only the pH promoter was sufficient to bring about high-level expression of pH in cardiac and skeletal muscle. Thus, while pH and pM function independently of each other, pN, remarkably, shares regulatory elements with each of them, depending on the tissue. Importantly, expression of the transgenes was independent of the integration site, as originally described for transgenes containing the beta-globin locus control region.

scholarly journals High level expression of transgenes by use of 5^|^#8242;-untranslated region of the Arabidopsis thaliana arabinogalactan-protein 21 gene in dicotyledons

2012 ◽  
Vol 29 (3) ◽  
pp. 319-322 ◽  
Author(s):  
Takeshi Matsui ◽  
Hideyuki Matsuura ◽  
Kazutoshi Sawada ◽  
Eiji Takita ◽  
Satoko Kinjo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Untranslated Region ◽  
Arabinogalactan Protein ◽  
High Level Expression ◽  
High Level
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