Why Does Clostridium acetireducens Not Use Interspecies Hydrogen Transfer for Growth on Leucine?

1997 ◽  
Vol 35 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Laurence Girbal ◽  
Jóhann Örlygsson ◽  
Bert J. Reinders ◽  
Jan C. Gottschal
Keyword(s):  
Hydrogen Transfer ◽  
Interspecies Hydrogen Transfer

Multidimensional modelling to investigate interspecies hydrogen transfer in anaerobic biofilms

2006 ◽  
Vol 40 (16) ◽  
pp. 3099-3108 ◽  
Author(s):  
D.J. Batstone ◽  
C. Picioreanu ◽  
M.C.M van Loosdrecht
Keyword(s):  
Hydrogen Transfer ◽  
Interspecies Hydrogen Transfer ◽  
Multidimensional Modelling

scholarly journals Coaggregation Facilitates Interspecies Hydrogen Transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus

2005 ◽  
Vol 71 (12) ◽  
pp. 7838-7845 ◽  
Author(s):  
Shun'ichi Ishii ◽  
Tomoyuki Kosaka ◽  
Katsutoshi Hori ◽  
Yasuaki Hotta ◽  
Kazuya Watanabe
Keyword(s):  
Microscopic Observation ◽  
Hydrogen Transfer ◽  
Large Fraction ◽  
Physical Contact ◽  
Exponential Growth Phase ◽  
Interspecies Hydrogen Transfer ◽  
Intercellular Spaces ◽  
Propionate Oxidation ◽  
The Mean ◽  
Methanothermobacter Thermautotrophicus

ABSTRACT A thermophilic syntrophic bacterium, Pelotomaculum thermopropionicum strain SI, was grown in a monoculture or coculture with a hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus strain ΔH. Microscopic observation revealed that cells of each organism were dispersed in a monoculture independent of the growth substrate. In a coculture, however, these organisms coaggregated to different degrees depending on the substrate; namely, a large fraction of the cells coaggregated when they were grown on propionate, but relatively few cells coaggregated when they were grown on ethanol or 1-propanol. Field emission-scanning electron microscopy revealed that flagellum-like filaments of SI cells played a role in making contact with ΔH cells. Microscopic observation of aggregates also showed that extracellular polymeric substance-like structures were present in intercellular spaces. In order to evaluate the importance of coaggregation for syntrophic propionate oxidation, allowable average distances between SI and ΔH cells for accomplishing efficient interspecies hydrogen transfer were calculated by using Fick's diffusion law. The allowable distance for syntrophic propionate oxidation was estimated to be approximately 2 μm, while the allowable distances for ethanol and propanol oxidation were 16 μm and 32 μm, respectively. Considering that the mean cell-to-cell distance in the randomly dispersed culture was approximately 30 μm (at a concentration in the mid-exponential growth phase of the coculture of 5 × 107 cells ml−1), it is obvious that close physical contact of these organisms by coaggregation is indispensable for efficient syntrophic propionate oxidation.

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