Salt-Sensitive and Auxotrophic Mutants of Halomonas elongata and H. meridiana by Use of Hydroxylamine Mutagenesis

1997 ◽  
Vol 34 (2) ◽  
pp. 85-90 ◽  
Author(s):  
David Cánovas ◽  
Carmen Vargas ◽  
Antonio Ventosa ◽  
Joaquín J. Nieto
Keyword(s):  
Auxotrophic Mutants ◽  
Halomonas Elongata ◽  
Hydroxylamine Mutagenesis

scholarly journals Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes.

1998 ◽  
Vol 273 (19) ◽  
pp. 11986
Author(s):  
David Cánovas ◽  
Carmen Vargas ◽  
Fernando Iglesias-Guerra ◽  
Laszlo N. Csonka ◽  
David Rhodes ◽  
...  
Keyword(s):  
Moderate Halophile ◽  
Isolation And Characterization ◽  
Halomonas Elongata ◽  
Ectoine Synthesis

scholarly journals Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

Catalysts ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 520
Author(s):  
David Roura Padrosa ◽  
Zoya Nisar ◽  
Francesca Paradisi
Keyword(s):  
Continuous Flow ◽  
Membrane Reactors ◽  
Dialysis Membrane ◽  
Widespread Application ◽  
Recycling System ◽  
Alanine Dehydrogenase ◽  
Halomonas Elongata ◽  
Amino Donor ◽  
Recycling Systems ◽  
Excellent Stability

Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthesise amines with unfavourable equilibria. To circumvent this, recycling systems for the amino donor, such as amino acid dehydrogenases or aldolases, have proved useful to push the equilibria while avoiding high amino donor concentrations. In this work, we report the use of a new alanine dehydrogenase from the halotolerant bacteria Halomonas elongata which exhibits excellent stability to different cosolvents, combined with the well characterised CbFDH as a recycling system of L-alanine for the amination of three model substrates with unfavourable equilibria. In a step forward, the amino donor recycling system has been co-immobilised and used in flow with success as well as re-used as a dialysis enclosed system for the amination of an aromatic aldehyde.

scholarly journals Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

1995 ◽  
Vol 52 (3) ◽  
pp. 548-554 ◽  
Author(s):  
V. Kava - Cordeiro ◽  
E.A. Luna - Alves - Lima ◽  
J.L. Azevedo
Keyword(s):  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Nitrous Acid ◽  
Wild Strain ◽  
Wild Type ◽  
Survival Curves ◽  
Auxotrophic Mutants ◽  
Morphological Mutants

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

scholarly journals Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

2016 ◽  
Vol 14 (39) ◽  
pp. 9306-9311 ◽  
Author(s):  
Martina Letizia Contente ◽  
Matteo Planchestainer ◽  
Francesco Molinari ◽  
Francesca Paradisi
Keyword(s):  
Stereoelectronic Effects ◽  
Aromatic Substrates ◽  
Halomonas Elongata

scholarly journals Protection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and In Vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model

2006 ◽  
Vol 74 (11) ◽  
pp. 6491-6495 ◽  
Author(s):  
Sunhee Lee ◽  
Bo-Young Jeon ◽  
Svetoslav Bardarov ◽  
Mei Chen ◽  
Sheldon L. Morris ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Glutamine Synthetase ◽  
Mycobacterium Bovis ◽  
Murine Model ◽  
Triple Mutant ◽  
Auxotrophic Mutants ◽  
Bcg Vaccination ◽  
In Vivo Growth ◽  
Subcutaneous Immunization

ABSTRACT We generated four individual glutamine synthetase (GS) mutants (ΔglnA1, ΔglnA2, ΔglnA3, and ΔglnA4) and one triple mutant (ΔglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

Production of carminomycin by auxotrophic mutants of Actinomadura carminata

1975 ◽  
Vol 15 (5) ◽  
pp. 383-385 ◽  
Author(s):  
O. A. Lapchinskaya ◽  
T. A. Saburova ◽  
V. I. Ponomarenko
Keyword(s):  
Auxotrophic Mutants
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