News & Notes: Isolation of Ewingella americana from the Cultivated Mushroom, Agaricus bisporus

1996 ◽  
Vol 33 (5) ◽  
pp. 334-337 ◽  
Author(s):  
Peter W. Inglis ◽  
John F. Peberdy
Keyword(s):  
Agaricus Bisporus ◽  
Ewingella Americana ◽  
Cultivated Mushroom

scholarly journals Cloning of a chitinase gene from Ewingella americana, a pathogen of the cultivated mushroom, Agaricus bisporus

2000 ◽  
Vol 23 (3) ◽  
pp. 685-688 ◽  
Author(s):  
P.W. Inglis ◽  
J.F. Peberdy ◽  
R.E. Sockett
Keyword(s):  
Agaricus Bisporus ◽  
Genomic Library ◽  
Signal Sequence ◽  
Selective Medium ◽  
Peptide Sequence ◽  
Accession Number ◽  
Gene Encoding ◽  
Expression Screening ◽  
Ewingella Americana ◽  
Cultivated Mushroom

We have isolated a gene encoding a chitinase (EC 3.2.1.14) from Ewingella americana, a recently described pathogen of the mushroom Agaricus bisporus. This gene, designated chiA (EMBL/Genbank/DDBJ accession number X90562), was cloned by expression screening of a plasmid-based E. americana HindIII genomic library in Escherichia coli using remazol brilliant violet-stained carboxymethylated chitin incorporated into selective medium. The chiA gene has a 918-bp ORF, terminated by a TAA codon, with a calculated polypeptide size of 33.2 kDa, likely corresponding to a previously purified and characterised 33-kDa endochitinase from E. americana. The deduced amino acid sequence shares 33% identity with chitinase II from Aeromonas sp. No. 10S-24 and 7.8% identity with a chitinase from Saccharopolyspora erythraeus. Homology to other chitinase sequences was otherwise low. The peptide sequence deduced from chiA lacks a typical N-terminal signal sequence and also lacks the chitin binding and type III fibronectin homology units common to many bacterial chitinases. The possibility that this chitinase is not primarily adapted for the environmental mineralisation of pre-formed chitin, but rather for the breakdown of nascent chitin, is discussed in the context of mushroom disease.

Accumulation and distribution of selenium and cesium in the cultivated mushroom Agaricus bisporus — A radiotracer-aided study

Chemosphere ◽  
1998 ◽  
Vol 36 (8) ◽  
pp. 1787-1798 ◽  
Author(s):  
Johannes T. van Elteren ◽  
Urszula D. Woroniecka ◽  
Koos J. Kroon
Keyword(s):  
Agaricus Bisporus ◽  
Cultivated Mushroom

A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus

1982 ◽  
Vol 52 (1) ◽  
pp. 43-48 ◽  
Author(s):  
W.C. Wong ◽  
J.T. Fletcher ◽  
B.A. Unsworth ◽  
T.F. Preece
Keyword(s):  
Agaricus Bisporus ◽  
Bacterial Disease ◽  
Cultivated Mushroom

scholarly journals Identification of two laccase genes in the cultivated mushroom Agaricus bisporus

1993 ◽  
Vol 139 (6) ◽  
pp. 1209-1218 ◽  
Author(s):  
C. R. Perry ◽  
M. Smith ◽  
C. H. Britnell ◽  
D. A. Wood ◽  
C. F. Thurston
Keyword(s):  
Agaricus Bisporus ◽  
Cultivated Mushroom ◽  
Laccase Genes

Aggressiveness of green mould on cultivated mushroom (Agaricus bisporus) in Turkey

2020 ◽  
Vol 127 (5) ◽  
pp. 695-708 ◽  
Author(s):  
Mehmet Aydoğdu ◽  
İlker Kurbetli ◽  
Aytül Kitapçı ◽  
Görkem Sülü
Keyword(s):  
Agaricus Bisporus ◽  
Cultivated Mushroom ◽  
Green Mould

scholarly journals Resistance of Pre- and Post-epidemic Strains of Agaricus bisporus to Trichoderma aggressivum f. aggressivum

Plant Disease ◽  
2003 ◽  
Vol 87 (12) ◽  
pp. 1457-1461 ◽  
Author(s):  
X. Chen ◽  
M. D. Ospina-Giraldo ◽  
V. Wilkinson ◽  
D. J. Royse ◽  
C. P. Romaine
Keyword(s):  
North America ◽  
Agaricus Bisporus ◽  
Disease Susceptibility ◽  
Protection Assay ◽  
Complementary Methods ◽  
Green Mold ◽  
Methods Of Analysis ◽  
Cultivated Mushroom ◽  
Cultivated Mushrooms ◽  
Grain Protection

Since the early 1990s, the epidemic of green mold on the cultivated mushroom Agaricus bisporus in North America has been caused by Trichoderma aggressivum f. aggressivum. The findings of earlier research suggested that the microevolutionary emergence of T. aggressivum f. aggressivum coincided with the onset of the epidemic. This hypothesis was tested further by determining the disease susceptibility of mushroom strains grown widely before the epidemic manifested. The results of complementary methods of analysis, which entailed a grain protection assay and cropping trials, established that two pre-epidemic strains were more susceptible to green mold than three post-epidemic strains being cultivated at the time of the epidemic. Thus, if T. aggressivum f. aggressivum had been present within cultivated mushrooms prior to the epidemic, it should have been detected. It still appears to be true that T. aggressivum f. aggressivum emerged during the 1990s in a manner that remains unclear.

Chromosomal Abnormalities Associated with Strain Degeneration in the Cultivated Mushroom,Agaricus bisporus

1996 ◽  
Vol 20 (3) ◽  
pp. 229-241 ◽  
Author(s):  
Paul A. Horgen ◽  
Daisy Carvalho ◽  
Anton Sonnenberg ◽  
Aimin Li ◽  
L.J.L.D. Van Griensven
Keyword(s):  
Agaricus Bisporus ◽  
Chromosomal Abnormalities ◽  
Cultivated Mushroom
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