Role of Type 1 Fimbriae in the Adhesion of Escherichia coli to Salivary Mucin and Secretory Immunoglobulin A

1996 ◽  
Vol 33 (3) ◽  
pp. 200-208 ◽  
Author(s):  
Alice Moshier ◽  
Molakala S. Reddy
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Type 1 Fimbriae ◽  
Salivary Mucin ◽  
Secretory Immunoglobulin

scholarly journals Secretory immunoglobulin A carries oligosaccharide receptors for Escherichia coli type 1 fimbrial lectin.

1990 ◽  
Vol 58 (9) ◽  
pp. 3073-3077 ◽  
Author(s):  
A E Wold ◽  
J Mestecky ◽  
M Tomana ◽  
A Kobata ◽  
H Ohbayashi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

scholarly journals Reduced Phase Switch Capacity and Functional Adhesin Expression of Type 1-Fimbriated Escherichia coli from Immunoglobulin A-Deficient Individuals

2006 ◽  
Vol 75 (2) ◽  
pp. 932-940 ◽  
Author(s):  
Forough L. Nowrouzian ◽  
Vanda Friman ◽  
Ingegerd Adlerberth ◽  
Agnes E. Wold
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Carriage Rate ◽  
E Coli ◽  
Frequent Failure ◽  
Colonic Cells ◽  
Secretory Immunoglobulin ◽  
Carbohydrate Chains

ABSTRACT The mannose-specific adhesin of type 1 fimbriae is the most common adhesin in Escherichia coli. One receptor for this adhesin is the carbohydrate chains of secretory immunoglobulin A (S-IgA), and intestinal E. coli from IgA-deficient individuals has a reduced capacity to adhere to mannose-containing receptors. Here, we investigated the expression of the mannose-specific adhesin and its capacity to switch to the fimbriated phenotype in colonic resident and transient E. coli strains isolated from control (n = 16) and IgA-deficient (n = 17) persons. Resident E. coli strains from IgA-deficient individuals displayed weaker mannose-specific adherence to colonic cells than resident strains from control individuals (21 versus 44 bacteria/cell, P = 0.0009) due to three mechanisms: a lower carriage rate of the fimH gene (90% versus 97%, not significant), more frequent failure to switch on the fim genes (30% versus 6%, P = 0.02), and the reduced adhesive potential of fimH + isolates capable of phase switch (26 versus 46 bacteria/cell, P = 0.02). On the other hand, resident strains from IgA-deficient individuals displayed stronger mannose-resistant adherence than resident strains from control individuals (P = 0.04) and transient strains from IgA-deficient individuals (P = 0.01). The presence of S-IgA appears to favor the establishment of E. coli clones which readily express mannose-specific adhesins in the bowel microbiota.

Inhibition of enteroaggregative Escherichia coli adhesion to HEp-2 cells by secretory immunoglobulin A from human colostrum

2001 ◽  
Vol 20 (7) ◽  
pp. 672-678 ◽  
Author(s):  
ROSELICE MARIO FERNANDES ◽  
SOLANGE BARROS CARBONARE ◽  
MAGDA MARIA SALLES CARNEIRO-SAMPAIO ◽  
LUIZ RACHID TRABULSI
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
Human Colostrum ◽  
Secretory Immunoglobulin

scholarly journals Fab-Independent Antiadhesion Effects of Secretory Immunoglobulin A on S-Fimbriated Escherichia coli Are Mediated by Sialyloligosaccharides

1998 ◽  
Vol 66 (8) ◽  
pp. 3971-3973 ◽  
Author(s):  
Horst Schroten ◽  
Christoph Stapper ◽  
Ricarda Plogmann ◽  
Henrik Köhler ◽  
Jörg Hacker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Human Milk ◽  
Immunoglobulin A ◽  
Secretory Immunoglobulin A ◽  
E Coli ◽  
Human Epithelial Cells ◽  
Early Human ◽  
Secretory Immunoglobulin

ABSTRACT S-fimbriated Escherichia coli strains cause sepsis and meningitis in newborns and are known to recognize the carbohydrate sequence sialyl-(α2-3)-galactoside. We show that adhesion of cloned S-fimbriated E. coli to human epithelial cells is inhibited Fab independently by sialyloligosaccharides on secretory immunoglobulin A (s-IgA). This indicates an anti-infective function of s-IgA (Fc), particularly in early human milk.

scholarly journals Escherichia coli type-1 fimbriae are critical to overcome initial bottlenecks of infection upon low-dose inoculation in a porcine model of cystitis

Microbiology ◽  
2021 ◽  
Vol 167 (10) ◽  
Author(s):  
Kristian Stærk ◽  
Rasmus Birkholm Grønnemose ◽  
Thomas Kastberg Nielsen ◽  
Nicky Anúel Petersen ◽  
Yaseelan Palarasah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Porcine Model ◽  
Post Inoculation ◽  
Upec Strain ◽  
Content Type ◽  
Type 1 Fimbriae ◽  
Susceptibility To Infection ◽  
General Importance

Most uropathogenic Escherichia coli (UPEC) express type-1 fimbriae (T1F), a key virulence factor for urinary tract infection (UTI) in mice. Evidence that conclusively associates this pilus with uropathogenesis in humans has, however, been difficult to obtain. We used an experimental porcine model of cystitis to assess the role of T1F in larger mammals more closely related to humans. Thirty-one pigs were infected with UPEC strain UTI89 or its T1F deficient mutant, UTI89ΔfimH, at inoculum titres of 102 to 108 colony forming units per millilitre. Urine and blood samples were collected and analysed 7 and 14 days post-inoculation, and whole bladders were removed at day 14 and analysed for uroepithelium-associated UPEC. All animals were consistently infected and reached high urine titres independent of inoculum titre. UTI89ΔfimH successfully colonized the bladders of 1/6 pigs compared to 6/6 for the wild-type strain. Intracellular UPEC were detectable in low numbers in whole bladder explants. In conclusion, low doses of UPEC are able to establish robust infections in pigs, similar to what is presumed in humans. T1F are critical for UPEC to surpass initial bottlenecks during infection but may be dispensable once infection is established. While supporting the conclusions from mice studies regarding a general importance of T1F in successfully infecting the host, the porcine UTI models’ natural high, more human-like, susceptibility to infection, allowed us to demonstrate a pivotal role of T1F in initial establishment of infection upon a realistic low-inoculum introduction of UPEC in the bladder.

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