Substrate Specificity of the Highly Alkalophilic Bacterial Proteinase Esperase: Relation to the X-Ray Structure

2001 ◽  
Vol 42 (5) ◽  
pp. 368-371 ◽  
Author(s):  
Dessislava Nikolova Georgieva ◽  
Stanka Stoeva ◽  
Wolfgang Voelter ◽  
Nicolay Genov ◽  
Christian Betzel
Keyword(s):  
Substrate Specificity ◽  
X Ray ◽  
Bacterial Proteinase

scholarly journals Substrate specificity of three new intradiol dioxygenases: an X-ray characterization

2005 ◽  
Vol 61 (a1) ◽  
pp. c209-c209 ◽  
Author(s):  
M. Ferraroni ◽  
V. M. Travkin ◽  
M. P. Kolomytseva ◽  
A. Scozzafava ◽  
L. Golovleva ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
X Ray

X-ray Structure, Bioinformatics Analysis, and Substrate Specificity of a 6-Phospho-β-glucosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis

2020 ◽  
Vol 60 (12) ◽  
pp. 6392-6407
Author(s):  
Wayde Veldman ◽  
Marcelo Vizona Liberato ◽  
Vitor M. Almeida ◽  
Valquiria P. Souza ◽  
Maira A. Frutuoso ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Bacillus Licheniformis ◽  
Glycoside Hydrolase ◽  
Bioinformatics Analysis ◽  
X Ray

Chemo-enzymatic Synthesis of 3′-azido/-amino-C-4′-spirooxetano-xylo nucleosides

2020 ◽  
Vol 7 (1) ◽  
pp. 120-127 ◽  
Author(s):  
Manish Kumar ◽  
Rajesh Kumar ◽  
Neha Rana ◽  
Ashok K. Prasad
Keyword(s):  
Substrate Specificity ◽  
Ethyl Acetate ◽  
Enzymatic Synthesis ◽  
Environmentally Friendly ◽  
Modified Nucleosides ◽  
Quantitative Yield ◽  
Hydroxymethyl Group ◽  
Crucial Step ◽  
X Ray ◽  
Sugar Derivative

Conformationally locked 3'-azido-C-4'-spirooxetano-xylonucleosides T, U, C and A have been synthesized by following chemo-enzymatic convergent route. One of the 3'-azido-C-4'- spirooxetano-xylonucleosides, i.e. T was converted into 3'-amino-C-4'-spirooxetano-xylothymidine by reduction of azide to amine with H2/Pd-C in ethyl acetate in quantitative yield. The crucial step in the synthesis of spirooxetano-xylonucleosides is the Lipozyme® TL IM-mediated exclusive diastereoselective acetylation of 4-C-hydroxymethyl group in dihydroxysugar derivative, 3-azido-3-deoxy-4-Chydroxymethyl- 1,2-O-isopropylidene-α-D-xylofuranose in quantitative yield. The diastereoselective monoacetylation of 4-C-hydroxymethyl in dihydroxysugar derivative was unambiguously confirmed by X-ray crystal study on the tosylated compound obtained by the tosylation of Lipozyme® TL IM - mediated monoacetylated sugar derivative. The broader substrate specificity and exclusive selective nature of Lipozyme® TL IM can be utilised for the development of environmentally friendly methodologies for the synthesis of different sugar-modified nucleosides of importance.

scholarly journals X-ray Analysis of the NMC-A β-Lactamase at 1.64-Å Resolution, a Class A Carbapenemase with Broad Substrate Specificity

1998 ◽  
Vol 273 (41) ◽  
pp. 26714-26721 ◽  
Author(s):  
Peter Swarén ◽  
Laurent Maveyraud ◽  
Xavier Raquet ◽  
Stéphanie Cabantous ◽  
Colette Duez ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Broad Substrate Specificity ◽  
X Ray ◽  
Class A

scholarly journals X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity

2012 ◽  
Vol 40 (20) ◽  
pp. 10554-10566 ◽  
Author(s):  
Akira Hirata ◽  
Kosuke Fujishima ◽  
Ryota Yamagami ◽  
Takuya Kawamura ◽  
Jillian F. Banfield ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Broad Substrate Specificity ◽  
X Ray ◽  
Trna Splicing ◽  
Fourth Type

scholarly journals Expansion of Substrate Specificity and Catalytic Mechanism of Azoreductase by X-ray Crystallography and Site-directed Mutagenesis

2008 ◽  
Vol 283 (20) ◽  
pp. 13889-13896 ◽  
Author(s):  
Kosuke Ito ◽  
Masayuki Nakanishi ◽  
Woo-Cheol Lee ◽  
Yuehua Zhi ◽  
Hiroshi Sasaki ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Catalytic Mechanism ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
X Ray ◽  
X Ray Crystallography
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