Real-Time PCR Identification of Six Malassezia Species

2017 ◽  
Vol 74 (6) ◽  
pp. 671-677 ◽  
Author(s):  
Amin Ilahi ◽  
Inès Hadrich ◽  
Sourour Neji ◽  
Houaida Trabelsi ◽  
Fattouma Makni ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Malassezia Species ◽  
Pcr Identification

scholarly journals Multi-Probe Real-Time PCR Identification of Common Mycobacterium Species in Blood Culture Broth

2009 ◽  
Vol 11 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Suporn Foongladda ◽  
Suporn Pholwat ◽  
Boonchuay Eampokalap ◽  
Pattarachai Kiratisin ◽  
Ruengpung Sutthent
Keyword(s):  
Blood Culture ◽  
Real Time ◽  
Real Time Pcr ◽  
Culture Broth ◽  
Mycobacterium Species ◽  
Pcr Identification

Qualitative real-time PCR identification of the khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae)

2018 ◽  
Vol 54 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Satoshi Furui ◽  
Akihiro Miyanoshita ◽  
Taro Imamura ◽  
Yasutaka Minegishi ◽  
Ryota Kokutani
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Trogoderma Granarium ◽  
Pcr Identification ◽  
Khapra Beetle

Validation of the TadpoleTMCampylobacter jejuni Real-Time PCR Identification Kit

2019 ◽  
Vol 102 (3) ◽  
pp. 842-854
Author(s):  
Yuan Jiang ◽  
Jinling Shen ◽  
Feng Xue ◽  
Lina Zhao ◽  
Jielin Yang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Target Genes ◽  
Accurate Method ◽  
Common Species ◽  
Stability Study ◽  
Time Saving ◽  
Columbia Blood Agar ◽  
Negative Results ◽  
Pcr Identification

Abstract Background: The gene-based real-time PCR method for identification of Campylobacter jejuni is more simple, rapid and accurate than the traditional biochemical method. Objective: A performance validation of the TadpoleTMCampylobacter jejuni Real-Time PCR Identification Kit was performed. Method: The assay uses TaqMan Real-time PCR technology to amplify target genes from isolated colonies. Bacterial deoxyribonucleic acid (DNA) from inclusivity and exclusivity organisms cultured on Columbia Blood Agar, Campy-Cefex agar and modified Charcoal Cefoperazone Deoxycholate was extracted and analyzed on three instruments: Applied Biosystems (ABI) 7500 Fast, ABI StepOne Plus and Bio-Rad CFX96. Results: When 57 distinct strains of C. jejuni were tested for inclusivity, all 57 strains produced positive results on the three instruments. In exclusivity testing, all 35 strains of related organisms, including 7 non-target Campylobacter strains and other common species, produced negative results on the three instruments. The Independent Laboratory validation consisting of an inclusivity and exclusivity evaluation for 10 C. jejuni isolates and 10 nontarget Campylobacter isolates also showed 100% expected results on the three instruments. In addition, in robustness testing, small, deliberate changes to the assay parameters, including cell suspension turbidity, heat lysis time, and DNA template volume in the PCR reaction, did not affect the kit performance. Finally, the combined lot-to-lot and stability study on both the ABI 7500 Fast and the ABI StepOne Plus showed that the 11 C. jejuni strains and 5 nontarget Campylobacter strains can be correctly identified by the three independently manufactured, lots and it supported a shelf life of 9 months when stored at –20°C. Conclusions: The Tadpole method offers a rapid, accurate, and robust alternative for C. jejuni identification. Highlights: Rapid and accurate method to identify C. jejuni, which has a good robustness and high stability. It is flexible and offers the advantages of reduced labor and time saving.

scholarly journals Real-time PCR identification and detection of Fuscoporia torulosa in Quercus ilex

2007 ◽  
Vol 0 (0) ◽  
pp. 070924013950002-???
Author(s):  
G. Campanile ◽  
L. Schena ◽  
N. Luisi
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quercus Ilex ◽  
Pcr Identification
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