Characterization of Bacillus thuringiensis Strain DOR4 Toxic to Castor Semilooper Achaea janata: Proteolytic Processing and Binding of Toxins to Receptors

2008 ◽  
Vol 57 (1) ◽  
pp. 72-77
Author(s):  
Madhusudhan Budatha ◽  
Gargi Meur ◽  
P. S. Vimala Devi ◽  
P. B. Kirti ◽  
Aparna Dutta-Gupta
Keyword(s):  
Bacillus Thuringiensis ◽  
Proteolytic Processing ◽  
Achaea Janata ◽  
Castor Semilooper ◽  
Thuringiensis Strain

Characterization of the anti-cancer-cell parasporal proteins of aBacillus thuringiensisisolate

2000 ◽  
Vol 46 (10) ◽  
pp. 913-919 ◽  
Author(s):  
Satoko Yamashita ◽  
Tetsuyuki Akao ◽  
Eiichi Mizuki ◽  
Hiroyuki Saitoh ◽  
Kazuhiko Higuchi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cell ◽  
Human Cancer ◽  
Proteolytic Processing ◽  
Proteinase K ◽  
Major Protein ◽  
Human Cancer Cell ◽  
Soil Isolate ◽  
Anti Cancer

An unusual activity, associated with non-insecticidal and non-haemolytic parasporal inclusion proteins of a Bacillus thuringiensis soil isolate, designated 89-T-26-17, was characterized. The parasporal inclusion of this isolate was bipyramidal, rounded at both ends, containing proteins of 180, 150, 120, 100, and 88 kDa. No homologies with the Cry and Cyt proteins of B. thuringiensis were detected based on N-terminal sequences. Proteolytic processing of the inclusion proteins by proteinase K, trypsin, and chymotrypsin produced a major protein of 64 kDa exhibiting cytocidal activity against human leukaemic T cells and uterus cervix cancer (HeLa) cells. The protease-activated proteins showed no cytotoxicity to normal T cells.Key words: Bacillus thuringiensis parasporal inclusion, non-insecticidal, non-haemolytic, cytocidal activity, human cancer cell.

Cloning and Characterization of Two Novel Crystal Protein Genes, cry54Aa1 and cry30Fa1, from Bacillus thuringiensis Strain BtMC28

2009 ◽  
Vol 58 (6) ◽  
pp. 654-659 ◽  
Author(s):  
Furong Tan ◽  
Jun Zhu ◽  
Jie Tang ◽  
Xueming Tang ◽  
Shiquan Wang ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Protein ◽  
Thuringiensis Strain ◽  
Crystal Protein Genes

scholarly journals Differential expression and characterization of cypermethrin-degrading potential proteins in Bacillus thuringiensis strain, SG4

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Pankaj ◽  
Geeta Negi ◽  
Saurabh Gangola ◽  
Priyanka Khati ◽  
Govind Kumar ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Differential Expression ◽  
Thuringiensis Strain

Characterization of cry9Da4, cry9Eb2, and cry9Ee1 genes from Bacillus thuringiensis strain T03B001

2013 ◽  
Vol 97 (22) ◽  
pp. 9705-9713 ◽  
Author(s):  
Changlong Shu ◽  
Huiqin Su ◽  
Jie Zhang ◽  
Kanglai He ◽  
Dafang Huang ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Thuringiensis Strain
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