Differences in rhodamine-123 efflux in B-type chronic lymphocytic leukemia suggest possible gender and stage variations in drug-resistance gene activity

1998 ◽  
Vol 76 (5) ◽  
pp. 189-194 ◽  
Author(s):  
H. Steiner ◽  
A. Polliack ◽  
C. Kimchi-Sarfaty ◽  
D. Libster ◽  
E. Fibach ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Chronic Lymphocytic Leukemia ◽  
Resistance Gene ◽  
Lymphocytic Leukemia ◽  
Gene Activity ◽  
Rhodamine 123 ◽  
Drug Resistance Gene

The association of a genetic variant in multi-drug resistance gene and colorectal cancer susceptibility

Gene Reports ◽  
2021 ◽  
pp. 101252
Author(s):  
Forouzan Amerizadeh ◽  
Mehrdad Moetamani-Ahmadi ◽  
Soodabeh Shahidsales ◽  
Farzad Rahmani ◽  
Masoumeh Gharib ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Resistance ◽  
Resistance Gene ◽  
Genetic Variant ◽  
Cancer Susceptibility ◽  
Multi Drug Resistance ◽  
Drug Resistance Gene

Abstract 5135: Genome-wide CRISPR-based screening reveals ABCG2 as a novel drug resistance gene in pancreatic cancer

2019 ◽  
Author(s):  
Ryne C. Ramaker ◽  
Andrew A. Hardigan ◽  
Emily R. Gordon ◽  
Richard M. Myers ◽  
Sara J. Cooper
Keyword(s):  
Pancreatic Cancer ◽  
Drug Resistance ◽  
Resistance Gene ◽  
Drug Resistance Gene ◽  
Genome Wide ◽  
Novel Drug

scholarly journals Gene Therapy for Chronic Myelogenous Leukemia (CML): A Retroviral Vector That Renders Hematopoietic Progenitors Methotrexate-Resistant and CML Progenitors Functionally Normal and Nontumorigenic In Vivo

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4687-4698 ◽  
Author(s):  
Robert C.H. Zhao ◽  
R. Scott McIvor ◽  
James D. Griffin ◽  
Catherine M. Verfaillie
Keyword(s):  
Drug Resistance ◽  
Resistance Gene ◽  
Chronic Myelogenous Leukemia ◽  
Retroviral Vector ◽  
Normal Function ◽  
Myelogenous Leukemia ◽  
Dhfr Gene ◽  
Drug Resistance Gene ◽  
Cd34 Cells

Abstract Chronic myelogenous leukemia (CML) is a malignant disease of the human hematopoietic stem cell caused by the BCR/ABL gene rearrangement. The only curative therapy is allogeneic transplantation. Although autologous transplants may prolong survival, most patients relapse because of disease persisting in the host and in the graft. Continued administration of chemotherapy after transplant could reduce the incidence of relapse provided that the autograft can be protected by transfer of a drug-resistance gene. However, CML autografts will almost certainly contain malignant stem cells that will also be rendered drug-resistant. The presence of the BCR/ABL oncoprotein is necessary and sufficient for malignant transformation seen in CML. We thus hypothesized that transfer of a vector that combines a drug-resistance gene with anti-BCR/ABL antisense (AS) sequences may allow for posttransplant chemotherapy to decrease persistent disease while rendering inadvertently transduced CML stem and progenitor cells functionally normal. We constructed a retroviral vector, LasBD, that combines the methotrexate (MTX)-resistant tyrosine-22 dihydrofolate-reductase (tyr22-DHFR) gene and AS sequences directed at the b3a2 BCR/ABL breakpoint. b3a2 BCR/ABL containing 32D and MO7e cells were transduced with LasBD and selected in MTX for 14 days. Expression of the AS sequences reduced BCR/ABL mRNA and p210BCR/ABL protein levels by 6- to 10-fold in most cells. This subsequently led to the restoration of normal function of BCR/ABL cDNA+ cells: they grew significantly slower in the presence of interleukin-3 (IL-3); they underwent apoptotic cell death when cultured without IL-3; and they had restored expression and function of adhesion receptors. These effects were specific, because LasBD-containing AS sequences directed at the b3a2 BCR/ABL breakpoint did not affect p190BCR/ABL-containing cells. LasBD also rendered 20% to 30% of primary Ph− and Ph+ CD34+ cells MTX-resistant and decreased BCR/ABL mRNA levels in MTX resistant Ph+ CD34+ cells by 10-fold. Expression of the MTX-resistant DHFR gene and the AS sequences has been stable for at least 1 year in vitro and for more than 70 days in vivo. Finally, LasBD decreased tumorigenicity of 32DBCR/ABL cells in vivo by 3 to 4 logs. In conclusion, the tyr22-DHFR gene in the LasBD vector can protect normal hematopoietic cells from MTX-mediated toxicity, whereas the AS sequences in LasBD can suppress expression of the BCR/ABL gene and restore normal function of BCR/ABL cDNA-containing cells. The LasBD vector may therefore prove to be an extremely useful adjunct in autologous transplantation for CML.

Sign in / Sign up

Export Citation Format

Share Document