Human scFv antibody fragments specific for the epithelial tumour marker MUC-1, selected by phage display on living cells

2001 ◽  
Vol 50 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Cindy Wong ◽  
Robert Waibel ◽  
Michael Sheets ◽  
Jean-Pierre Mach ◽  
Ricarda Finnern
Keyword(s):  
Phage Display ◽  
Tumour Marker ◽  
Living Cells ◽  
Antibody Fragments ◽  
Scfv Antibody ◽  
Human Scfv ◽  
Epithelial Tumour

scholarly journals Selection of scFv Antibody Fragments Binding to Human Blood versus Lymphatic Endothelial Surface Antigens by Direct Cell Phage Display

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0127169 ◽  
Author(s):  
Thomas Keller ◽  
Romana Kalt ◽  
Ingrid Raab ◽  
Helga Schachner ◽  
Corina Mayrhofer ◽  
...  
Keyword(s):  
Phage Display ◽  
Human Blood ◽  
Surface Antigens ◽  
Antibody Fragments ◽  
Scfv Antibody ◽  
Endothelial Surface ◽  
Direct Cell ◽  
Selection Of

scholarly journals Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume

2021 ◽  
Author(s):  
K. K. Khaing ◽  
K. Rangnoi ◽  
H. Michlits ◽  
N. Boonkerd ◽  
N. Teaumroong ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Scfv Antibody ◽  
Nitrogen Fixing ◽  
Phage Display Technology ◽  
Human Scfv ◽  
Display Technology

Human scFv antibody generated from phage display technology was successfully used for the generation of specific recombinant antibodies: yiN92-1e10 and yiDOA9-162 for the detection of Bradyrhizobium strains SUTN9-2 and DOA9, respectively.

Isolation of soluble scFv antibody fragments specific for small biomarker molecule, L-Carnitine, using phage display

2016 ◽  
Vol 428 ◽  
pp. 9-19 ◽  
Author(s):  
Rabab M. Abou El-Magd ◽  
Nicolas F. Vozza ◽  
Jack A. Tuszynski ◽  
David S. Wishart
Keyword(s):  
Phage Display ◽  
Antibody Fragments ◽  
Scfv Antibody ◽  
Soluble Scfv

scholarly journals Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Shadman ◽  
Safar Farajnia ◽  
Mohammad Pazhang ◽  
Mohammadreza Tohidkia ◽  
Leila Rahbarnia ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
High Reactivity ◽  
Scfv Antibody ◽  
Phage Library ◽  
Human Antibody ◽  
Exotoxin A ◽  
Phage Elisa ◽  
Human Scfv ◽  
Domain I

Abstract Background Pseudomonas aeruginosa is the leading cause of nosocomial infections, especially in people with a compromised immune system. Targeting virulence factors by neutralizing antibodies is a novel paradigm for the treatment of antibiotic-resistant pseudomonas infections. In this respect, exotoxin A is one of the most potent virulence factors in P. aeruginosa. The present study was carried out to identify a novel human scFv antibody against the P. aeruginosa exotoxin A domain I (ExoA-DI) from a human scFv phage library. Methods The recombinant ExoA-DI of P. aeruginosa was expressed in E. coli, purified by Ni-NTA column, and used for screening of human antibody phage library. A novel screening procedure was conducted to prevent the elimination of rare specific clones. The phage clone with high reactivity was evaluated by ELISA and western blot. Results Based on the results of polyclonal phage ELISA, the fifth round of biopanning leads to the isolation of several ExoA-DI reactive clones. One positive clone with high affinity was selected by monoclonal phage ELISA and used for antibody expression. The purified scFv showed high reactivity with the recombinant domain I and full-length native exotoxin A. Conclusions The purified anti-exotoxin A scFv displayed high specificity against exotoxin A. The human scFv identified in this study could be the groundwork for developing a novel therapeutic agent to control P. aeruginosa infections.

scholarly journals Construction of human ScFv phage display library against ovarian tumor

2006 ◽  
Vol 26 (5) ◽  
pp. 497-499 ◽  
Author(s):  
Jinsong Xia ◽  
Hao Bi ◽  
Qin Yao ◽  
Shen Qu ◽  
Yiqiang Zong
Keyword(s):  
Phage Display ◽  
Ovarian Tumor ◽  
Phage Display Library ◽  
Human Scfv
Sign in / Sign up

Export Citation Format

Share Document