Expression of human prostate-specific antigen (PSA) in a mouse tumor cell line reduces tumorigenicity and elicits PSA-specific cytotoxic T lymphocytes

1996 ◽  
Vol 42 (6) ◽  
pp. 362-368 ◽  
Author(s):  
Chungwen Wei ◽  
Eugene Storozynsky ◽  
A. J. McAdam ◽  
Kun-Yun Yeh ◽  
Brian R. Tilton ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cell ◽  
Cell Line ◽  
Prostate Specific Antigen ◽  
Cytotoxic T Lymphocytes ◽  
Specific Antigen ◽  
Tumor Cell Line ◽  
Human Prostate ◽  
Mouse Tumor

Cytotoxic T lymphocytes against the antigen-processing-defective RMA-S tumor cell line

1992 ◽  
Vol 22 (6) ◽  
pp. 1639-1642 ◽  
Author(s):  
Alice J. A. M. Sijts ◽  
Marloes L. H. De Bruijn ◽  
John D. Nieland ◽  
W. Martin Kast ◽  
Cornelis J. M. Melief
Keyword(s):  
T Lymphocytes ◽  
Tumor Cell ◽  
Cell Line ◽  
Cytotoxic T Lymphocytes ◽  
Antigen Processing ◽  
Tumor Cell Line

scholarly journals Effect of emoxipine on cytotoxicity of peripheral blood mononuclears under cultivation with cytarabine and cyclocytidine

2021 ◽  
pp. 3-10
Author(s):  
Darya B. Nizheharodava ◽  
Marina M. Zafranskaya ◽  
Eugenii I. Kvasyuk ◽  
Aliaksei G. Sysa
Keyword(s):  
T Lymphocytes ◽  
Tumor Cell ◽  
Cell Line ◽  
Peripheral Blood ◽  
Tumor Cell Line ◽  
Lymphoid Cells ◽  
Modified Nucleosides ◽  
Special Role ◽  
Peripheral Blood Mononuclears

Taking into account the special role of oxidative stress that increases during cancer chemotherapy, the effect of the antioxidant emoxipine on peripheral blood mononuclears was studied under conditions that simulate the cytotoxic effects of antimetabolites of a number of modified cytidine nucleosides in relation to the tumor cell line K562. Lymphoid cells were also a source for subsequent modelling of the immune response to the cancer. It was found that neither the modified nucleosides themselves nor their combination with emoxipine caused changes in IL-2-stimulated cytotoxicity of lymphoid cells in relation to K562 tumor cell line. A study of the expression of the CD107a marker showed a significant stimulating effect of 1 µmol/L of citarabine on the activation of subpopulations of T-lymphocytes (CD3+ ) and cytotoxic T-lymphocytes (CD3+ CD8+ ).

Generation of tumor-specific cytotoxic T-lymphocytes from peripheral blood of colorectal cancer patients for adoptive immunotherapy.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 3056-3056
Author(s):  
Marco Bregni ◽  
Serena Del Bue ◽  
Andrea Galli ◽  
Alberto della Valle ◽  
Pasquale Ferrante ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cytotoxic T Lymphocytes ◽  
Peripheral Blood ◽  
Tumor Cell Line ◽  
Interleukin 4 ◽  
Autologous Tumor ◽  
The Third ◽  
Ifn Γ

3056 Background: Adoptive T-cell transfer (ACT) using autologous TIL, grown ex vivo and then infused into the cancer patient after lymphoablative chemotherapy, has emerged as an effective treatment for patients with metastatic melanoma. However, this approach has been hampered by the difficulty of isolating TILs from tumors other than melanoma, and of amplifying a sufficient quantity of autologous tumor-reactive T cells. So we decided to adopt a recently described procedure for generating in vitro large numbers of anti-tumor HLA-restricted CTLs, by stimulating patient’s CD8-enriched peripheral blood mononuclear cells (PBMCs) with DCs pulsed with apoptotic solid tumor cells (TCs) as a source of tumor antigens. Methods: 61 patients affected by CRC were enrolled. Tumor biopsies were obtained at surgery, together with 100 ml of heparinized peripheral blood. Tumors were dissociated to a single-cell suspension and cultured in order to obtain tumor cell line from each patient. Dendritic cells (DCs) were generated from previously separated PBMCs, using a magnetic positive selection of CD14+ monocytes, cultured in presence of Interleukin-4 and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF). Anti-tumor cytotoxic T lymphocytes (CTLs) were elicited using DCs as antigen-presenting cells, autologous apoptotic tumor cells as source of antigens and T CD-8 lymphocytes enriched effectors, with weekly stimulation. To evaluate the cytotoxic activity of CTLs, interferon-γ (IFN-γ) secretion was assessed by ELISPOT. Results: Tumor cell lines and DCs were obtained from 19 out of 61 patients. ELISPOT was performed so far for 6 patients: strong IFN-γ secretion was detected at the third, fourth and fifth stimulations for one patient and at the second for another patient, whereas for three patients a weak secretion was detected during the second and the third stimulations. CTLs from one patient did not react to the stimulations. Conclusions: Generation of CTLs suitable for ACT immunotherapy is feasible from peripheral blood in patients with CRC.

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