Evidence that acidic fibroblast growth factor promotes maturation of rat satellite-cell-derived myotubes in vitro

1999 ◽  
Vol 65 (3) ◽  
pp. 161 ◽  
Author(s):  
Sabine Düsterhöft ◽  
D. Pette
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Satellite Cell ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor

In vitro lens transdifferentiation of Xenopus laevis outer cornea induced by Fibroblast Growth Factor (FGF)

Development ◽  
1997 ◽  
Vol 124 (2) ◽  
pp. 421-428
Author(s):  
L. Bosco ◽  
G. Venturini ◽  
D. Willems
Keyword(s):  
Growth Factor ◽  
Xenopus Laevis ◽  
Fibroblast Growth Factor ◽  
Specific Inhibitor ◽  
Bovine Brain ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Lens Fibers

It has been shown that lens regeneration from outer cornea of larval Xenopus laevis is dependent on neural retina both in vivo and in tissue culture. The isolated outer cornea cultured in the presence of bovine brain-derived acidic Fibroblast Growth Factor (aFGF) is able to reprogram the differentiation into lens fibers, although this transdifferentiative process is not coupled with the formation of a normally organized lens. The capacity of aFGF to promote lens differentiation from cornea is not linked to its mitogenic activity. The cultured corneal cells can transdifferentiate into lens fibers in the presence of aFGF when DNA replication and cell proliferation are prevented by addition of aphidicolin, a specific inhibitor of DNA polymerase in eukaryotes, to the culture medium.

scholarly journals Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor

1998 ◽  
Vol 336 (1) ◽  
pp. 213-222 ◽  
Author(s):  
Elona KOLPAKOVA ◽  
Antoni WIĘDŁOCHA ◽  
Harald STENMARK ◽  
Olav KLINGENBERG ◽  
Pål Ø. FALNES ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
High Efficiency ◽  
Signal Sequence ◽  
Binding Protein ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Reading Frame ◽  
Intracellular Binding

In addition to its extracellular action, there is evidence that acidic fibroblast growth factor (aFGF) acts inside cells. To identify intracellular proteins interacting with aFGF, we screened a HeLa cell library in the yeast two-hybrid system using pLex-aFGF as a bait. A clone binding to aFGF, but not to the non-mitogenic mutant aFGF-K132E, was isolated and characterized. The insert contained an open reading frame corresponding to a novel protein of 42 kDa. The protein, termed aFGF intracellular binding protein (FIBP), is mainly hydrophilic and does not contain an N-terminal signal sequence. In vitro-translated FIBP bound specifically to a fusion protein of maltose-binding protein and aFGF. FIBP became post-translationally associated with microsomes added to the cell-free protein synthesizing system, and the membrane-associated protein bound aFGF with high efficiency. Immunoblots and fluorescence microscopy demonstrated that the protein is present in nuclei and, to a lesser extent, associated with mitochondria and other cytoplasmic membranes. The possibility is discussed that FIBP may be involved in the mitogenic action of aFGF. The nucleotide sequences described in this paper have been submitted to GeneBank database under accession numbers AF010187 (human FIBP) and AF010188 (simian FIBP).

Activities of Human Acidic Fibroblast Growth Factor in an In Vitro Dermal Equivalent Model

1991 ◽  
Vol 97 (5) ◽  
pp. 793-798 ◽  
Author(s):  
Louis. Dubertret ◽  
Françoise. Brunner-Ferber ◽  
John. Misiti ◽  
Kenneth A. Thomas ◽  
Marie-Liesse. Dubertret
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Equivalent Model ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Dermal Equivalent
Sign in / Sign up

Export Citation Format

Share Document