A set of Hansenula polymorpha integrative vectors to construct lacZ fusions

1999 ◽  
Vol 53 (1) ◽  
pp. 23-29 ◽  
Author(s):  
N. Brito ◽  
M. D. Pérez ◽  
G. Perdomo ◽  
C. González ◽  
P. García-Lugo ◽  
...  
Keyword(s):  
Hansenula Polymorpha ◽  
Lacz Fusions ◽  
Integrative Vectors

Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions

Gene ◽  
1986 ◽  
Vol 45 (3) ◽  
pp. 299-310 ◽  
Author(s):  
Alan M. Myers ◽  
Alexander Tzagoloff ◽  
Dennis M. Kinney ◽  
Carol J. Lusty
Keyword(s):  
Lacz Fusions ◽  
Integrative Vectors ◽  
Multiple Cloning Sites

scholarly journals A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae

2018 ◽  
Vol 3 ◽  
pp. 72
Author(s):  
Peter W Daniels ◽  
Anuradha Mukherjee ◽  
Alastair SH Goldman ◽  
Bin Hu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Manipulation ◽  
Strand Break ◽  
Integration Strategy ◽  
System A ◽  
Target Dna ◽  
Biosynthesis Gene ◽  
Dna Fragment ◽  
Integrative Vectors ◽  
Yeast Genomes

Integrating a desired DNA sequence into yeast genomes is a widely-used genetic manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration method is to use an integrative plasmid such as pRS or YIplac series as the target DNA carrier. The nature of this method risks multiple integrations of the target DNA and the potential loss of integrated DNA during cell proliferation. In this study, we developed a novel yeast integration strategy based on the widely used CRISPR-Cas9 system and created a set of plasmids for this purpose. In this system, a plasmid bearing Cas9 and gRNA expression cassettes will induce a double-strand break (DSB) inside a biosynthesis gene such as Met15 or Lys2. Repair of the DSB will be mediated by another plasmid bearing upstream and downstream sequences of the DSB and an integration sequence in between. As a result of this repair the sequence is integrated into genome by replacing the biosynthesis gene, the disruption of which leads to a new auxotrophic genotype. The newly-generated auxotroph can serve as a traceable marker for the integration. In this study, we demonstrated that a DNA fragment up to 6.3 kb can be efficiently integrated into the Met15 or Lys2 locus using this system. This novel integration strategy can be applied to various yeasts, including natural yeast isolated from wild environments or different yeast species such as Candida albicans.

Investigations on the possible role of the endoplasmic reticulum in the development of peroxisomes in the yeast Hansenula polymorpha

1985 ◽  
Vol 51 (5-6) ◽  
pp. 572-572
Author(s):  
M. Veenhuis ◽  
A. C. Douma ◽  
P. G. Bruinenberg ◽  
W. Harder
Keyword(s):  
Endoplasmic Reticulum ◽  
Hansenula Polymorpha

scholarly journals Different structures of selected and unselected araB–lacZ fusions

1997 ◽  
Vol 23 (6) ◽  
pp. 1133-1145 ◽  
Author(s):  
Genevieve Maenhaut-Michel ◽  
Catherine E. Blake ◽  
David R. F. Leach ◽  
James A. Shapiro
Keyword(s):  
Lacz Fusions

scholarly journals Watermelon glyoxysomal malate dehydrogenase is sorted to peroxisomes of the methylotrophic yeast, Hansenula polymorpha

FEBS Letters ◽  
1993 ◽  
Vol 334 (1) ◽  
pp. 128-132 ◽  
Author(s):  
I.J. van der Klei ◽  
K.N. Faber ◽  
I. Keizer-Gunnink ◽  
C. Gietl ◽  
W. Harder ◽  
...  
Keyword(s):  
Malate Dehydrogenase ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast
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