Preparation of stearoyl lactic acid ester catalyzed by lipases from Rhizomucor miehei and porcine pancreas optimization using response surface methodology

1999 ◽  
Vol 52 (4) ◽  
pp. 579-584 ◽  
Author(s):  
K. R. Kiran ◽  
N. G. Karanth ◽  
S. Divakar
Keyword(s):  
Response Surface Methodology ◽  
Lactic Acid ◽  
Response Surface ◽  
Acid Ester ◽  
Rhizomucor Miehei ◽  
Porcine Pancreas

scholarly journals Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 831 ◽  
Author(s):  
Juan Calero ◽  
Diego Luna ◽  
Carlos Luna ◽  
Felipa Bautista ◽  
Beatriz Hurtado ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Silica Gel ◽  
Rhizomucor Miehei ◽  
Rhizomucor Miehei Lipase ◽  
Reaction Parameters ◽  
Experimental Conditions ◽  
Inorganic Solids ◽  
Lipozyme Rm Im ◽  
Miehei Lipase

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.

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