Modulation of gene expression by (CA) n microsatellites in the filamentous ascomycete Podospora anserina

1999 ◽  
Vol 52 (2) ◽  
pp. 191-195 ◽  
Author(s):  
A. Khashnobish ◽  
A. Hamann ◽  
H. D. Osiewacz
Keyword(s):  
Gene Expression ◽  
Podospora Anserina ◽  
Filamentous Ascomycete ◽  
Modulation Of Gene Expression

scholarly journals Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach

2020 ◽  
Vol 117 (31) ◽  
pp. 18424-18430 ◽  
Author(s):  
Emily K. Bowman ◽  
Matthew Deaner ◽  
Jan-Fang Cheng ◽  
Robert Evans ◽  
Ernst Oberortner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Essential Genes ◽  
Yeast Gene ◽  
Promoter Regions ◽  
Guide Rna ◽  
Metabolic Genes ◽  
Complementary Approach ◽  
Modulation Of Gene Expression ◽  
Generation Sequencing

Most classic genetic approaches utilize binary modifications that preclude the identification of key knockdowns for essential genes or other targets that only require moderate modulation. As a complementary approach to these classic genetic methods, we describe a plasmid-based library methodology that affords bidirectional, graded modulation of gene expression enabled by tiling the promoter regions of all 969 genes that comprise the ito977 model ofSaccharomyces cerevisiae’s metabolic network. When coupled with a CRISPR-dCas9–based modulation and next-generation sequencing, this method affords a library-based, bidirection titration of gene expression across all major metabolic genes. We utilized this approach in two case studies: growth enrichment on alternative sugars, glycerol and galactose, and chemical overproduction of betaxanthins, leading to the identification of unique gene targets. In particular, we identify essential genes and other targets that were missed by classic genetic approaches.

Resveratrol Modulation of Gene Expression

2005 ◽  
pp. 167-191
Author(s):  
Valeria Cucciolla ◽  
Adriana Borriello ◽  
Vincenzo Zappia ◽  
Fulvio Della Ragione
Keyword(s):  
Gene Expression ◽  
Modulation Of Gene Expression

Resveratrol Modulation of Gene Expression: The Role of Transcription Factors

2005 ◽  
pp. 168-191
Author(s):  
Fulvio Della Ragione ◽  
Valeria Cucciolla ◽  
Adriana Borriello ◽  
Vincenzo Zappia
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Modulation Of Gene Expression
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