The role of the alternative respiratory pathway in the stimulation of cephalosporin C formation by soybean oil in Acremonium chrysogenum

1999 ◽  
Vol 51 (5) ◽  
pp. 633-638 ◽  
Author(s):  
L. Karaffa ◽  
E. Sándor ◽  
J. Kozma ◽  
C. P. Kubicek ◽  
A. Szentirmai
Keyword(s):  
Soybean Oil ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway ◽  
Stimulation Of

The Role of Cu in Respiration of Pea Plants and Heterotrophically Growing Scenedesmus Cells

1988 ◽  
Vol 43 (5-6) ◽  
pp. 438-442 ◽  
Author(s):  
Matilde Barón Ayala ◽  
Gerhard Sandmann
Keyword(s):  
Cytochrome Oxidase ◽  
Respiratory Activity ◽  
The Other ◽  
Nadh Oxidation ◽  
Alternative Respiration ◽  
Respiratory Pathway ◽  
Cu Deficiency ◽  
Alternative Respiratory Pathway

In Scenedesmus about half of NADH oxidation proceeds via a cyanide-sensitive and the other half via a cyanide-insensitive respiratory pathway. In contrast, respiration is completely cyanide sensitive in pea indicating that the alternative respiratory pathway is absent. Cu deficiency in pea plants and in heterotrophically grown Scenedesmus cells interferes with respiratory activity of mitochondria. In both organisms, the cyanide-sensitive NADH oxidation was strongly decreased during cultivation in low Cu media. Cu sensitivity was also observed for the alternative respiratory pathway in Scenedesmus. These results suggest that a Cu-containing component is involved in the alternative respiratory pathway. This is the main reason why alternative respiration cannot be regarded as a compensation for low cytochrome-oxidase activities during Cu starvation. The Cu dependency of the cyanide-sensitive respiration was located at the site of cytochrome oxidase. A strong coordination of the biosynthesis of the Cu-containing cytochrome-oxidase complex was evident. When the endogenous Cu pool was low, formation of cytochrome aa3, another component of cytochrome oxidase, was also decreased.

Stimulation of the cyanide-resistant alternative respiratory pathway by oxygen in Acremonium chrysogenum correlates with the size of the intracellular peroxide pool

2003 ◽  
Vol 49 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Levente Karaffa ◽  
Erzsébet Sándor ◽  
Erzsébet Fekete ◽  
József Kozma ◽  
Attila Szentirmai ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Respiratory Activity ◽  
Peroxyl Radical ◽  
Radical Scavenger ◽  
Acremonium Chrysogenum ◽  
Alternative Respiration ◽  
Respiratory Pathway ◽  
Submerged Cultures ◽  
Alternative Respiratory Pathway ◽  
The Relationship

The relationship between oxygen input and activity of the cyanide-resistant alternative respiration of submerged cultures of Acremonium crysogenum was investigated. The volumetric oxygen transfer coefficient of the respective cultures correlated positively within almost two ranges of magnitude with the size of the intracellular peroxide pool, which in turn, correlated with the activity of the cyanide-resistant alternative respiratory pathway. Increased aeration also stimulated the glucose uptake rate but had no effect on the total respiration rate or the growth rate. Addition of the lipid peroxyl radical scavenger DL-α-tocopherol to A. chrysogenum cultures decreased the rate of intracellular peroxide production as well as glucose uptake. An increase in the cyanide-resistant fraction of total respiration was observed, while growth and the total respiratory activity remained unchanged. We conclude that intracellular peroxides may stimulate the alternative respiration in A. chrysogenum.Key words: Acremonium chrysogenum, alternative respiration, oxygen, peroxide, Kla.

scholarly journals The critical role of plasma membrane H+-ATPase activity in cephalosporin C biosynthesis of Acremonium chrysogenum

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0238452 ◽  
Author(s):  
Alexander Zhgun ◽  
Mariya Dumina ◽  
Ayrat Valiakhmetov ◽  
Mikhail Eldarov
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Critical Role ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum

Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol

2010 ◽  
Vol 101 (12) ◽  
pp. 4549-4553 ◽  
Author(s):  
Hyun Yong Shin ◽  
Jin Young Lee ◽  
You Ree Jung ◽  
Seung Wook Kim
Keyword(s):  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Cephalosporin C Production ◽  
Stimulation Of

Performance improvement of cephalosporin C fermentation by Acremonium chrysogenum with DO-Stat based strategy of co-feeding soybean oil and glucose

2013 ◽  
Vol 48 (12) ◽  
pp. 1822-1830 ◽  
Author(s):  
Hongzhen Luo ◽  
Jingshu Zhang ◽  
Guoqiang Yuan ◽  
Yanli Zhao ◽  
Han Liu ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Performance Improvement ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum

scholarly journals Temperature-dependent alterations of respiration in leaves of two selected vascular plant species: the role of the alternative oxidase pathway

Bragantia ◽  
2002 ◽  
Vol 61 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Natalia V. Pystina ◽  
Roman A. Danilov
Keyword(s):  
Alternative Oxidase ◽  
Alternative Pathway ◽  
Vascular Plant ◽  
Relative Activity ◽  
Temperature Dependent ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway ◽  
Benzhydroxamic Acid ◽  
Increasing Temperature

Effects of higher temperatures on respiration and activity of alternative oxidase (AOX) were studied in mature leaves of Ajuga reptans L. and Rhodiola rosea L. Total respiration in both A. reptans and R. rosea increased exponentially with the increasing temperature of 10 °C to 35 °C. Respiration in the presence of benzhydroxamic acid (BHAM) also increased exponentially in accordance with the increasing temperature in the leaves of both A. reptans and R. rosea. Relative activity of the alternative pathway decreased significantly in the leaves of A. reptans with increasing temperatures. However, an increase in the relative activity of the alternative pathway was detected in the leaves of R. rosea. Thermoresistance of the alternative respiratory pathway was considered to be higher in R. rosea compared to A. reptans. We suppose that A. reptans and R. rosea have different mechanisms regulating partitioning of electrons to the alternative respiratory pathway.

Stimulation of Ca(2+)-dependent exocytosis of the sperm acrosome by cAMP acting downstream of phospholipase A2

Reproduction ◽  
2000 ◽  
pp. 57-68 ◽  
Author(s):  
J Garde ◽  
ER Roldan
Keyword(s):  
Arachidonic Acid ◽  
Phospholipase A2 ◽  
Phospholipase C ◽  
Phosphodiesterase Inhibitors ◽  
Dibutyryl Camp ◽  
Camp Phosphodiesterase ◽  
Ram Sperm ◽  
Camp Formation ◽  
Stimulation Of

Spermatozoa undergo exocytosis in response to agonists that induce Ca2+ influx and, in turn, activation of phosphoinositidase C, phospholipase C, phospholipase A2, and cAMP formation. Since the role of cAMP downstream of Ca2+ influx is unknown, this study investigated whether cAMP modulates phospholipase C or phospholipase A2 using a ram sperm model stimulated with A23187 and Ca2+. Exposure to dibutyryl-cAMP, phosphodiesterase inhibitors or forskolin resulted in enhancement of exocytosis. However, the effect was not due to stimulation of phospholipase C or phospholipase A2: in spermatozoa prelabelled with [3H]palmitic acid or [14C]arachidonic acid, these reagents did not enhance [3H]diacylglycerol formation or [14C]arachidonic acid release. Spermatozoa were treated with the phospholipase A2 inhibitor aristolochic acid, and dibutyryl-cAMP to test whether cAMP acts downstream of phospholipase A2. Under these conditions, exocytosis did not occur in response to A23187 and Ca2+. However, inclusion of dibutyryl-cAMP and the phospholipase A2 metabolite lysophosphatidylcholine did result in exocytosis (at an extent similar to that seen when cells were treated with A23187/Ca2+ and without the inhibitor). Inclusion of lysophosphatidylcholine alone, without dibutyryl-cAMP, enhanced exocytosis to a lesser extent, demonstrating that cAMP requires a phospholipase A2 metabolite to stimulate the final stages of exocytosis. These results indicate that cAMP may act downstream of phospholipase A2, exerting a regulatory role in the exocytosis triggered by physiological agonists.

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