Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon, randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates

1999 ◽  
Vol 51 (1) ◽  
pp. 91-99 ◽  
Author(s):  
D. Corroler ◽  
N. Desmasures ◽  
M. Gueguen
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Polymerase Chain Reaction Analysis ◽  
Phenotypic Characterization ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Histidine Biosynthesis ◽  
Polymerase Chain

scholarly journals A Micro Polymerase Chain Reaction Module for Integrated and Portable DNA Analysis Systems

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Elisa Morganti ◽  
Cristian Collini ◽  
Cristina Potrich ◽  
Cristina Ress ◽  
Andrea Adami ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Thermal Characterization ◽  
Chain Reaction ◽  
Element Analysis ◽  
Thermal Requirements ◽  
Chip Carrier ◽  
Polymerase Chain ◽  
Analysis System

This work deals with the design, fabrication, and thermal characterization of a disposable miniaturized Polymerase Chain Reaction (PCR) module that will be integrated in a portable and fast DNA analysis system. It is composed of two independent parts: a silicon substrate with embedded heater and thermometers and a PDMS (PolyDiMethylSiloxane) chamber reactor as disposable element; the contact between the two parts is assured by a mechanical clamping obtained using a Plastic Leaded Chip Carrier (PLCC). This PLCC is also useful, avoid the PCR mix evaporation during the thermal cycles. Finite Element Analysis was used to evaluate the thermal requirements of the device. The thermal behaviour of the device was characterized revealing that the temperature can be controlled with a precision of ±0.5°C. Different concentrations of carbon nanopowder were mixed to the PDMS curing agent in order to increase the PDMS thermal conductivity and so the temperature control accuracy.

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