Characterization of a novel Streptococcus thermophilus rolling-circle plasmid used for vector construction

1998 ◽  
Vol 50 (2) ◽  
pp. 174-180 ◽  
Author(s):  
D. K. Y. Solaiman ◽  
G. A. Somkuti
Keyword(s):  
Streptococcus Thermophilus ◽  
Rolling Circle ◽  
Vector Construction

scholarly journals Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes

2006 ◽  
Vol 80 (7) ◽  
pp. 3523-3531 ◽  
Author(s):  
Reimar Johne ◽  
Walter Wittig ◽  
Daniel Fernández-de-Luco ◽  
Ursula Höfle ◽  
Hermann Müller
Keyword(s):  
Rolling Circle Amplification ◽  
Large Tumor ◽  
Rolling Circle ◽  
Reading Frame ◽  
Field Samples ◽  
Close Relationship ◽  
Avian Polyomavirus ◽  
Avian Group ◽  
Pyrrhula Pyrrhula

ABSTRACT Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds—avian polyomavirus (APV) and goose hemorrhagic polyomavirus (GHPV)—are the primary agents of acute and chronic disease with high mortality rates in young birds. Screening of field samples of diseased birds by consensus PCR revealed the presence of two novel polyomaviruses in the liver of an Eurasian bullfinch (Pyrrhula pyrrhula griseiventris) and in the spleen of a Eurasian jackdaw (Corvus monedula), tentatively designated as finch polyomavirus (FPyV) and crow polyomavirus (CPyV), respectively. The genomes of the viruses were amplified by using multiply primed rolling-circle amplification and cloned. Analysis of the FPyV and CPyV genome sequences revealed a close relationship to APV and GHPV, indicating the existence of a distinct avian group among the polyomaviruses. The main characteristics of this group are (i) involvement in fatal disease, (ii) the existence of an additional open reading frame in the 5′ region of the late mRNAs, and (iii) a different manner of DNA binding of the large tumor antigen compared to that of the mammalian polyomaviruses.

scholarly journals CHARACTERIZATION OF HAZELNUT MILK FERMENTED BY LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS AND STREPTOCOCCUS THERMOPHILUS

2018 ◽  
pp. 677-686 ◽  
Author(s):  
Ertan Ermiş ◽  
Rabia Güneş ◽  
İnci Zent ◽  
Muhammed Yusuf Çağlar ◽  
Mustafa Tahsin Yılmaz
Keyword(s):  
Streptococcus Thermophilus ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Delbrueckii Subsp

scholarly journals Massive sequencing of artisan cheeses from raw sheep’s milk

2019 ◽  
Vol 6 (5) ◽  
pp. 236-240
Author(s):  
Ruiz Julia ◽  
Etcheverria Analia ◽  
Padola Nora ◽  
Kruger Alejandra ◽  
Medina Luis
Keyword(s):  
Dairy Products ◽  
Food Industry ◽  
Streptococcus Thermophilus ◽  
Molecular Techniques ◽  
Native Flora ◽  
Three Samples ◽  
Massive Sequencing ◽  
Traditional Fermentation ◽  
Antibacterial Capacity

Lactic acid bacteria (LAB) are used in the food industry to confer aromatic characteristics and their antibacterial capacity. In this study the native flora of LAB that participates in the traditional fermentation of semi-hard cheeses made with raw sheep's milk from the region of Andalusia, Spain was analyzed. Three samples of four different commercial cheeses were taken. Massive sequencing was carried out to identify the lactic and accompanying flora. Predominant lactic flora was Lactococcus lactis, Streptococcus thermophilus, Lactobacillus paracasei and Lactococcus raffinolactis, and to a lesser extent other species of the genera Lactobacillus, Streptococcus, Pediococcus and Leuconestoc. The accompanying flora was composed of species of the genera Mycoplasma, Pseudomonas, Acinetobacter, Chryseobacterium, Mannheimia, Trueperella, Enterococcus, Vibrio, Serratia, Macrococcus, Staphylococcus, Massilia, Flavobacterium, Yersinia, Gallaecimonas, Hafnia, Leclercia, Obesumbacterium, Morganella and Kluyvera. These results show that modern molecular techniques are very good tools to identify natural LABs of artisanal dairy products. The characterization of the native flora of the artisanal cheese allows us to evaluate the microbiological diversity of the natural population of LAB and the symbiosis with another type of flora.

scholarly journals Molecules and Metabolites from Natural Products as Inhibitors of Biofilm in Candida spp. pathogens

2019 ◽  
Vol 19 (28) ◽  
pp. 2567-2578 ◽  
Author(s):  
Rajeev K. Singla ◽  
Ashok K. Dubey
Keyword(s):  
Natural Products ◽  
Mechanism Of Action ◽  
Functional Characterization ◽  
Streptococcus Thermophilus ◽  
Antifungal Drugs ◽  
Bioactive Molecules ◽  
Review Of The Literature ◽  
Candida Spp ◽  
Biofilm Inhibition

Background: Biofilm is a critical virulence factor associated with the strains of Candida spp. pathogens as it confers significant resistance to the pathogen against antifungal drugs. Methods: A systematic review of the literature was undertaken by focusing on natural products, which have been reported to inhibit biofilms produced by Candida spp. The databases explored were from PubMed and Google Scholar. The abstracts and full text of the manuscripts from the literature were analyzed and included if found significant. Results: Medicinal plants from the order Lamiales, Apiales, Asterales, Myrtales, Sapindales, Acorales, Poales and Laurales were reported to inhibit the biofilms formed by Candida spp. From the microbiological sources, lactobacilli, Streptomyces chrestomyceticus and Streptococcus thermophilus B had shown the strong biofilm inhibition potential. Further, the diverse nature of the compounds from classes like terpenoids, phenylpropanoid, alkaloids, flavonoids, polyphenol, naphthoquinone and saponin was found to be significant in inhibiting the biofilm of Candida spp. Conclusion: Natural products from both plant and microbial origins have proven themselves as a goldmine for isolating the potential biofilm inhibitors with a specific or multi-locus mechanism of action. Structural and functional characterization of the bioactive molecules from active extracts should be the next line of approach along with the thorough exploration of the mechanism of action for the already identified bioactive molecules.

Characterization of the plasmid pMB1 from Bifidobacterium longum and its use for shuttle vector construction

1996 ◽  
Vol 147 (3) ◽  
pp. 133-143 ◽  
Author(s):  
M Rossi ◽  
P Brigidi ◽  
A Gonzalez Vara y Rodriguez ◽  
D Matteuzzi
Keyword(s):  
Shuttle Vector ◽  
Bifidobacterium Longum ◽  
Vector Construction

scholarly journals Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum

Plasmid ◽  
2014 ◽  
Vol 73 ◽  
pp. 1-9 ◽  
Author(s):  
Tannaz Jalilsood ◽  
Ali Baradaran ◽  
Foo Hooi Ling ◽  
Shuhaimi Mustafa ◽  
Khatijah Yusof ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Rolling Circle Replication ◽  
Rolling Circle
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