Extracellular laccase production during hyphal interactions between Trichoderma sp. and Shiitake, Lentinula edodes

1998 ◽  
Vol 49 (5) ◽  
pp. 589-593 ◽  
Author(s):  
J.-M. Savoie ◽  
G. Mata ◽  
C. Billette
Keyword(s):  
Lentinula Edodes ◽  
Laccase Production ◽  
Trichoderma Sp ◽  
Extracellular Laccase

scholarly journals Extracellular laccase production and phenolic degradation by an olive mill wastewater isolate

2018 ◽  
Vol 69 (1) ◽  
pp. 231 ◽  
Author(s):  
R. Kumar ◽  
Y. Raizner ◽  
L. I. Kruh ◽  
O. Menashe ◽  
H. Azaizeh ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Antimicrobial Agents ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Laccase Production ◽  
16S Rna ◽  
Extracellular Laccase ◽  
Acinetobacter Pittii ◽  
Constitutive Production ◽  
Olive Mill

Olive mill wastewater (OMWW) presents a challenge to the control of effluents due to the presence of a high organic load, antimicrobial agents (monomeric-polymeric phenols, volatile acids, polyalcohols, and tannins), salinity and acidity. In this study, the production of extracellular laccase, monomeric or polymeric phenol, from an OMWW isolate based on its ability to biodegrade phenols and gallic acid as a model of phenolic compounds in OMWW was investigated. Phylogenetic analysis of the 16S RNA gene sequences identified the bacterial isolate (Acinetobacter REY) as being closest to Acinetobacter pittii. This isolate exhibited a constitutive production of extracellular laccase with an activity of 1.5 and 1.3 U ml/L when supplemented with the inducers CuSO4 and CuSO4+phenols, respectively. Batch experiments containing minimal media supplemented with phenols or gallic acid as the sole carbon and energy source were performed in order to characterize their phenolic biodegradability. Acinetobacter REY was capable of biodegrading up to 200 mg/L of phenols and gallic acid both after 10 h and 72 h, respectively.

scholarly journals Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation

2011 ◽  
Vol 68 (3) ◽  
pp. 295-300 ◽  
Author(s):  
Érica Clarissa D'Agostini ◽  
Talita Rafaele D'Agostini Mantovani ◽  
Juliana Silveira do Valle ◽  
Luzia Doretto Paccola-Meirelles ◽  
Nelson Barros Colauto ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Lentinula Edodes ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Biofuel Production ◽  
Laccase Production ◽  
Plant Residues ◽  
Low Carbon ◽  
Protein Nitrogen ◽  
Hydrolysis Of

Basidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth and decreased laccase production. The highest activities were obtained with a C/N ratio of 5. P. ostreatus, L. edodes and A. blazei produced more laccase when AS, AS:U and U, respectively, were added. In addition, C/N ratios lower than 30 induced laccase syntheses, inhibited mycelial growth and were a better condition for pre-hydrolysis of plant residues.

scholarly journals The Nutritional Value Enhancement of Oil Palm Empty Fruit Bunches as Animal Feed Using the Fungus Coprinus Comatus, with Different Numbers of Inoculums and Incubation Times

2021 ◽  
Vol 16 (3) ◽  
pp. 269-274
Author(s):  
Sitti Sabariyah ◽  
Rusdi ◽  
Damry ◽  
Asriani Hasanuddin
Keyword(s):  
Oil Palm ◽  
Biological Treatment ◽  
Trametes Versicolor ◽  
Nutritional Value ◽  
Lentinula Edodes ◽  
Animal Feed ◽  
Coprinus Comatus ◽  
Empty Fruit Bunches ◽  
Trichoderma Sp ◽  
Degradation Ability

This study aimed to increase the nutritional value of oil palm empty bunches (EFB) as ruminant animal feed by using biological treatment. To achieve this, five fungi species were used, including Trametes Versicolor, Lentinula edodes, Coprinus comatus, Pleurotus sajor-caju, and Trichoderma sp, which were inoculated for 20 days. Furthermore, the study consisted of 2 stages, in the first, the five species were tested for their degradability to lignin. In the second, the results were analyzed for their degradation ability by treating several numbers of inoculums (0.5 ml, 0.75 ml, and 1.0 ml) at different incubation times (20, 30, and 40 days). The results showed that the fungi treatment gave different lignin levels of oil palm empty fruit bunches compared to others. Furthermore, treatment with Coprinus comatus fungi produced the lowest lignin and the highest cellulose levels than others. This species works well compared to other fungi in the delignification of oil palm empty fruit bunches. With the use of Coprinus comatus, the lowest lignin and highest cellulose levels were obtained in a 0.5 ml inoculum treatment and at 30 days incubation time, however, there was no interaction. Conclusively, this study indicated that the application of Coprinus comatus to oil palm empty fruit bunches reduces lignin levels and increases cellulose by 22.04% and 20%, respectively. Consequently, there is an improved nutritional value of oil palm empty fruit bunches.

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

Microbiology ◽  
2005 ◽  
Vol 151 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Charles Junghanns ◽  
Monika Moeder ◽  
Gudrun Krauss ◽  
Claudia Martin ◽  
Dietmar Schlosser
Keyword(s):  
Parent Compound ◽  
Laccase Production ◽  
Breakdown Product ◽  
Endocrine Activity ◽  
Water Contaminants ◽  
Endocrine Disrupting Compound ◽  
Lower Specificity ◽  
Extracellular Laccase ◽  
Endocrine Disrupting ◽  
Molecular Masses

Degradation of technical nonylphenol (t-NP), known as an endocrine-disrupting compound mixture, was assessed, using the mitosporic fungal strain UHH 1-6-18-4 isolated from nonylphenol-contaminated river water, and a strain of the aquatic hyphomycete Clavariopsis aquatica. GC-MS analysis could resolve 12 peaks attributable to nonyl chain-branched t-NP isomers. All were degraded, to individual extents. Analysis of degradation metabolites suggested intracellular hydroxylation of the nonyl moieties of individual t-NP isomers. Further metabolites also indicated shortening of branched nonyl chains, and 4-hydroxybenzoic acid was identified as a t-NP breakdown product in UHH 1-6-18-4. The t-NP degradation efficiency was higher in UHH 1-6-18-4 than in C. aquatica, and a lower specificity in degradation of individual t-NP constituents in UHH 1-6-18-4 than in C. aquatica was observed. Strain UHH 1-6-18-4 concomitantly produced extracellular laccase under degradation conditions. A mixture of CuSO4 and vanillic acid considerably enhanced laccase production in both fungi. Laccase preparations derived from UHH 1-6-18-4 and C. aquatica cultures also converted t-NP. Laccase-catalysed transformation of t-NP led to the formation of products with higher molecular masses than that of the parent compound. These results emphasize a role of fungi occurring in aquatic ecosystems in degradation of water contaminants with endocrine activity, which has not previously been considered. Furthermore, the results are in support of two different mechanisms employed by fungi isolated from aquatic environments to initiate t-NP degradation: hydroxylation of individual t-NP isomers at their branched nonyl chains and further breakdown of the alkyl chains of certain isomers; and attack of t-NP by extracellular laccase, the latter leading to oxidative coupling of primary radical products to compounds with higher molecular masses.

Sign in / Sign up

Export Citation Format

Share Document