Patterns of ligninolytic enzymes in Trametes versicolor . Distribution of extra- and intracellular enzyme activities during cultivation on glucose, wheat straw and beech wood

1997 ◽  
Vol 47 (4) ◽  
pp. 412-418 ◽  
Author(s):  
D. Schlosser ◽  
R. Grey ◽  
W. Fritsche
Keyword(s):  
Wheat Straw ◽  
Enzyme Activities ◽  
Trametes Versicolor ◽  
Beech Wood ◽  
Ligninolytic Enzymes ◽  
Intracellular Enzyme

scholarly journals Role of laccase as an enzymatic pretreatment method to improve lignocellulosic saccharification

2014 ◽  
Vol 4 (8) ◽  
pp. 2251-2259 ◽  
Author(s):  
Lucy Heap ◽  
Anthony Green ◽  
David Brown ◽  
Bart van Dongen ◽  
Nicholas Turner
Keyword(s):  
Wheat Straw ◽  
Trametes Versicolor ◽  
Enzymatic Pretreatment ◽  
Pretreatment Method ◽  
Alkaline Peroxide

The saccharification of wheat straw was improved when an incubation step was performed withTrametes versicolorlaccase (TvL) and the mediator 1-hydroxybenzotriazole (1-HBT) prior to an alkaline peroxide extraction (APE).

scholarly journals Torrefaction of Woody and Agricultural Biomass: Influence of the Presence of Water Vapor in the Gaseous Atmosphere

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 30
Author(s):  
María González Martínez ◽  
Estéban Hélias ◽  
Gilles Ratel ◽  
Sébastien Thiéry ◽  
Thierry Melkior
Keyword(s):  
Water Vapor ◽  
Water Content ◽  
Wheat Straw ◽  
Beech Wood ◽  
High Water ◽  
High Water Content ◽  
Biomass Degradation ◽  
Moist Atmosphere ◽  
Thermochemical Transformation ◽  
Degradation Reactions

Biomass preheating in torrefaction at an industrial scale is possible through a direct contact with the hot gases released. However, their high water-content implies introducing moisture (around 20% v/v) in the torrefaction atmosphere, which may impact biomass thermochemical transformation. In this work, this situation was investigated for wheat straw, beech wood and pine forest residue in torrefaction in two complementary experimental devices. Firstly, experiments in chemical regime carried out in a thermogravimetric analyzer (TGA) showed that biomass degradation started from lower temperatures and was faster under a moist atmosphere (20% v/v water content) for all biomass samples. This suggests that moisture might promote biomass components’ degradation reactions from lower temperatures than those observed under a dry atmosphere. Furthermore, biomass inorganic composition might play a role in the extent of biomass degradation in torrefaction in the presence of moisture. Secondly, torrefaction experiments on a lab-scale device made possible to assess the influence of temperature and residence time under dry and 100% moist atmosphere. In this case, the difference in solid mass loss between dry and moist torrefaction was only significant for wheat straw. Globally, an effect of water vapor on biomass transformation through torrefaction was observed (maximum 10%db), which appeared to be dependent on the biomass type and composition.

scholarly journals Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Zichen Zhang ◽  
Aabid Manzoor Shah ◽  
Hassan Mohamed ◽  
Nino Tsiklauri ◽  
Yuanda Song
Keyword(s):  
Enzyme Activities ◽  
Dominant Role ◽  
Value Added ◽  
Ligninolytic Enzymes ◽  
Agricultural Wastes ◽  
Submerged Cultivation ◽  
X Ray Diffraction ◽  
Lignocellulose Conversion ◽  
Selective Delignification ◽  
Hydrolysis Of Cellulose

Lignocellulosic waste is the most abundant biorenewable biomass on earth, and its hydrolysis releases highly valued reducing sugars. However, the presence of lignin in the biopolymeric structure makes it highly resistant to solubilization thereby hindering the hydrolysis of cellulose and hemicellulose. Microorganisms are known for their potential complex enzymes that play a dominant role in lignocellulose conversion. Therefore, the current study was designed to isolate and screen potential microorganisms for their selective delignification ability for the pretreatment of lignocellulosic biomass. An extensive isolation and screening procedure yielded 36 desired isolates (22 bacteria, 7 basidiomycete fungi, and 7 filamentous fungi). Submerged cultivation of these desired microorganisms revealed 4 bacteria and 10 fungi with potent lignocellulolytic enzyme activities. The potent isolates were identified as Pleurotus, Trichoderma, Talaromyces, Bacillus, and Chryseobacterium spp. confirmed by morphological and molecular identification. The efficiency of these strains was determined through enzyme activities, and the degraded substrates were analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). Among all isolated microbes, Pleurotus spp. were found to have high laccase activity. The cellulose-decomposing and selective delignification strains were subjected to solid-state fermentation (SSF). SSF of field waste corn stalks as a single-carbon source provides Pleurotus spp. better condition for the secretion of ligninolytic enzymes. These isolated ligninolytic enzymes producing microorganisms may be used for the effective pretreatment of lignocellulosic agricultural wastes for the production of high value-added natural products by fermentation.

Sign in / Sign up

Export Citation Format

Share Document