Synthetic spider dragline silk proteins and their production in Escherichia coli

1997 ◽  
Vol 47 (1) ◽  
pp. 23-32 ◽  
Author(s):  
S. R. Fahnestock ◽  
S. L. Irwin
Keyword(s):  
Escherichia Coli ◽  
Silk Proteins ◽  
Dragline Silk ◽  
Spider Dragline Silk

scholarly journals Spider dragline silk proteins in transgenic tobacco leaves: accumulation and field production

2004 ◽  
Vol 2 (5) ◽  
pp. 431-438 ◽  
Author(s):  
Rima Menassa ◽  
Hong Zhu ◽  
Costas N. Karatzas ◽  
Anthoula Lazaris ◽  
Alex Richman ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Silk Proteins ◽  
Dragline Silk ◽  
Tobacco Leaves ◽  
Spider Dragline Silk ◽  
Field Production

scholarly journals Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183397 ◽  
Author(s):  
Ali D. Malay ◽  
Kazuharu Arakawa ◽  
Keiji Numata
Keyword(s):  
Amino Acid ◽  
Silk Proteins ◽  
Dragline Silk ◽  
Spider Dragline Silk

scholarly journals Novel Assembly Properties of Recombinant Spider Dragline Silk Proteins

2004 ◽  
Vol 14 (22) ◽  
pp. 2070-2074 ◽  
Author(s):  
Daniel Huemmerich ◽  
Thomas Scheibel ◽  
Fritz Vollrath ◽  
Shulamit Cohen ◽  
Uri Gat ◽  
...  
Keyword(s):  
Silk Proteins ◽  
Dragline Silk ◽  
Spider Dragline Silk

scholarly journals Super Bacteria: A New Hope of Manufacturing Spider Silk in an Efficient Way

2021 ◽  
Vol 8 (2) ◽  
pp. 225-226
Author(s):  
Chandrayee Talukdar ◽  
Swastik Sastri
Keyword(s):  
Escherichia Coli ◽  
Spider Silk ◽  
Huge Amount ◽  
Dragline Silk ◽  
Synthetic Genes ◽  
Spider Dragline Silk ◽  
Split Intein ◽  
Microbial System ◽  
Simple Product ◽  
Made In

The important properties of spider dragline silk and other protein polymers will find many applications. We have demonstrated the production of spider silk, which has many important properties, are produced from the bacteria including Escherichia coli. The productions of high molecular weight spider drag line encoded by synthetic genes. Silk protein can be efficiently produced by the microbial system has become an advantageous method like quick secretion and simple product recovery has become an efficient method .From the observation of various experiments done by several scientists has shown silk made in laboratory. The study of RIKEN centre for sustainable resource science has shown that spider silk can be produce huge amount. Observation shown that joining of the fragments by split intein sequence  which then cut itself to yield full name protein .Spun into fibers make the microbial spider silk tough , stretchable and stronger. Better modification of bioengineering can increase the amount of production.

Scalable Spider Silk Inspired Materials with High Extensibility and Super Toughness

2021 ◽  
Vol 893 ◽  
pp. 31-35
Author(s):  
Jin Lian Hu ◽  
Yuan Zhang Jiang ◽  
Lin Gu
Keyword(s):  
Chemical Synthesis ◽  
Spider Silk ◽  
Helical Structure ◽  
Silk Proteins ◽  
Dragline Silk ◽  
Spider Dragline Silk ◽  
Argiope Trifasciata ◽  
Chemical Synthesis Route ◽  
Β Sheet ◽  
Huge Challenge

Spiders silks have extraordinary strength and toughness simultaneously, thus has become dreamed materials by scientists and industries. Although there have been tremendous attempts to prepare fibers from genetically manufacture spider silk proteins, however, it has been still a huge challenge because of tedious procedure and high cost. Here, a facile spider-silk-mimicking strategy is reported for preparing highly scratchable polymers and supertough fibers from chemical synthesis route. Polymer films with high extensibility (>1200%) and supertough fibers (~387 MJ m-3) are achieved by introducing polypeptides with β-sheet and α-helical structure in polyureathane/urea polymers. Notabley,the toughness of the fiber is more than twice the reported value of a normal spider dragline silk, and comparable with the toughest spider silk, aciniform silk of Argiope trifasciata.

scholarly journals Antheraea pernyiSilk Fiber: A Potential Resource for Artificially Biospinning Spider Dragline Silk

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Yaopeng Zhang ◽  
Hongxia Yang ◽  
Huili Shao ◽  
Xuechao Hu
Keyword(s):  
Mechanical Properties ◽  
Optical Orientation ◽  
Silk Proteins ◽  
Dragline Silk ◽  
Silk Fibers ◽  
Spider Dragline Silk ◽  
Initial Modulus ◽  
Sheet Structure ◽  
Potential Resource ◽  
Breaking Energy

The outstanding properties of spider dragline silk are likely to be determined by a combination of the primary sequences and the secondary structure of the silk proteins.Antheraea pernyisilk has more similar sequences to spider dragline silk than the silk from its domestic counterpart,Bombyx mori. This makes it much potential as a resource for biospinning spider dragline silk. This paper further verified its possibility as the resource from the mechanical properties and the structures of theA. pernyisilks prepared by forcible reeling. It is surprising that the stress-strain curves of theA. pernyifibers show similar sigmoidal shape to those of spider dragline silk. Under a controlled reeling speed of 95 mm/s, the breaking energy was1.04×105 J/kg, the tensile strength was 639 MPa and the initial modulus was 9.9 GPa. It should be noted that this breaking energy of theA. pernyisilk approaches that of spider dragline silk. The tensile properties, the optical orientation and theβ-sheet structure contents of the silk fibers are remarkably increased by raising the spinning speeds up to 95 mm/s.

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