Purification and partial characterization of an elastinolytic proteinase from Aspergillus flavus culture filtrates

1996 ◽  
Vol 46 (2) ◽  
pp. 138-142 ◽  
Author(s):  
J. E. Mellon ◽  
P. J. Cotty
Keyword(s):  
Aspergillus Flavus ◽  
Partial Characterization ◽  
Culture Filtrates

PARTIAL CHARACTERIZATION OF SOIL HUMIC ACIDS THROUGH BIODEGRADATION

1967 ◽  
Vol 13 (5) ◽  
pp. 581-586 ◽  
Author(s):  
S. P. Mathur ◽  
E. A. Paul
Keyword(s):  
Amino Acids ◽  
Oxygen Tension ◽  
Humic Acids ◽  
Aromatic Amino Acids ◽  
Partial Characterization ◽  
Penicillium Frequentans ◽  
Enzyme Systems ◽  
Culture Filtrates ◽  
Partial Degradation

A strain of Penicillium frequentans was successfully employed for partial degradation and characterization of humic acids. Salicyl alcohol and salicylaldehyde were detected in culture filtrates of the fungus utilizing humates under reduced oxygen tension. The enzyme systems involved in the degradation of humic acids were adaptive. The humate-adapted mycelium was capable of metabolizing a number of compounds which occur in soil as products of degradation of lignin, aromatic amino acids, and plant glycosides but not polyphenolic hydrocarbons, resorcinol, and phloroglucinol.

A Maize Lectin-Like Protein with Antifungal Activity against Aspergillus flavus

2009 ◽  
Vol 72 (1) ◽  
pp. 120-127 ◽  
Author(s):  
R. L. BAKER ◽  
R. L. BROWN ◽  
Z.-Y. CHEN ◽  
T. E. CLEVELAND ◽  
A. M. FAKHOURY
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin Contamination ◽  
Partial Characterization ◽  
Ear Rot ◽  
Sources Of Resistance ◽  
Fungistatic Activity ◽  
Maize Genotypes ◽  
Proteomics Approach ◽  
Maize Kernels

The filamentous fungus Aspergillus flavus causes an ear rot on maize and produces a mycotoxin (aflatoxin) in colonized maize kernels. Aflatoxins are carcinogenic to humans and animals upon ingestion. Aflatoxin contamination results in a large loss of profits and marketable yields for farmers each year. Several research groups have worked to pinpoint sources of resistance to A. flavus and the resulting aflatoxin contamination in maize. Some maize genotypes exhibit greater resistance than others. A proteomics approach has recently been used to identify endogenous maize proteins that may be associated with resistance to the fungus. Research has been conducted on cloning, expression, and partial characterization of one such protein, which has a sequence similar to that of cold-regulated proteins. The expressed protein, ZmCORp, exhibited lectin-like hem-agglutination activity against fungal conidia and sheep erythrocytes. Quantitative real-time PCR assays revealed that ZmCOR is expressed 50% more in maize kernels from the Mp420 line, a type of maize resistant to A. flavus, compared with the expression level of the gene in the susceptible B73 line. ZmCORp exhibited fungistatic activity when conidia from A. flavus were exposed to the protein at a final concentration of 18 mM. ZmCORp inhibited the germination of conidia by 80%. A 50% decrease in mycelial growth resulted when germinated conidia were incubated with the protein. The partial characterization of ZmCORp suggests that this protein may play an important role in enhancing kernel resistance to A. flavus infection and aflatoxin accumulation.

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte
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