Organization and expression in Pseudomonas putida of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90

1996 ◽  
Vol 45 (4) ◽  
pp. 490-501 ◽  
Author(s):  
H. Kimura ◽  
H. Miyashita ◽  
Y. Sumino
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Lysobacter Lactamgenus

Cloning and characterization of a gene cluster involved in the catabolism of p-nitrophenol from Pseudomonas putida DLL-E4

2010 ◽  
Vol 101 (19) ◽  
pp. 7516-7522 ◽  
Author(s):  
Wenjing Shen ◽  
Weidong Liu ◽  
Jing Zhang ◽  
Jian Tao ◽  
Haihua Deng ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster

scholarly journals The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440

2019 ◽  
Vol 218 ◽  
pp. 1-11 ◽  
Author(s):  
Huizhong Liu ◽  
Huaduo Yan ◽  
Yujie Xiao ◽  
Hailing Nie ◽  
Qiaoyun Huang ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Pseudomonas Putida Kt2440

scholarly journals Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate.

1994 ◽  
Vol 176 (21) ◽  
pp. 6479-6488 ◽  
Author(s):  
C S Harwood ◽  
N N Nichols ◽  
M K Kim ◽  
J L Ditty ◽  
R E Parales
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster

Pseudomonas putida mutants in the exbBexbDtonB gene cluster are hypersensitive to environmental and chemical stressors

2004 ◽  
Vol 6 (6) ◽  
pp. 605-610 ◽  
Author(s):  
Patricia Godoy ◽  
Maria-Isabel Ramos-Gonzalez ◽  
Juan L. Ramos
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster

scholarly journals Genetic and functional characterization of the gene cluster directing the biosynthesis of putisolvin I and II in Pseudomonas putida strain PCL1445

Microbiology ◽  
2008 ◽  
Vol 154 (7) ◽  
pp. 2070-2083 ◽  
Author(s):  
Jean-Frédéric Dubern ◽  
Eric R. Coppoolse ◽  
Willem J. Stiekema ◽  
Guido V. Bloemberg
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Functional Characterization

scholarly journals Chromosomal Integration, Tandem Amplification, and Deamplification in Pseudomonas putida F1 of a 105-Kilobase Genetic Element Containing the Chlorocatechol Degradative Genes from Pseudomonas sp. Strain B13

1998 ◽  
Vol 180 (17) ◽  
pp. 4360-4369 ◽  
Author(s):  
Roald Ravatn ◽  
Sonja Studer ◽  
Dirk Springael ◽  
Alexander J. B. Zehnder ◽  
Jan Roelof van der Meer
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Pseudomonas Sp ◽  
Genetic Element ◽  
Structural Genes ◽  
Copy Numbers ◽  
Target Sites ◽  
Multiple Copies ◽  
Pseudomonas Putida F1 ◽  
Lower Copy

ABSTRACT Analysis of chlorobenzene-degrading transconjugants ofPseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) fromPseudomonas sp. strain B13 revealed that theclc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. putida RR22, a total of seven or eight chromosomal copies of the entire genetic element were present when the strain was cultivated on chlorobenzene. Chromosomal integrations of the 105-kb clc element occurred in two different loci, and the target sites were located within the 3′ end of glycine tRNA structural genes. Tandem amplification of theclc element was preferentially detected in one locus on the F1 chromosome. After prolonged growth on nonselective medium, transconjugant strain RR22 gradually diverged into subpopulations with lower copy numbers of the clc element. Two nonadjacent copies of the clc element in different loci always remained after deamplification, but strains with only two copies could no longer use chlorobenzene as a sole substrate. This result suggests that the presence of multiple copies of theclc gene cluster was a prerequisite for the growth ofP. putida RR22 on chlorobenzene and that amplification of the element was positively selected for in the presence of chlorobenzene.

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