Restriction endonuclease activity in Clostridium thermocellum and Clostridium thermosaccharolyticum

1996 ◽  
Vol 45 (1-2) ◽  
pp. 127-131 ◽  
Author(s):  
T. R. Klapatch ◽  
A. L. Demain ◽  
L. R. Lynd
Keyword(s):  
Restriction Endonuclease ◽  
Clostridium Thermocellum ◽  
Endonuclease Activity ◽  
Clostridium Thermosaccharolyticum

An Electrochemical Assay for Restriction Endonuclease Activity Using Graphene Monolayer

2014 ◽  
Vol 161 (12) ◽  
pp. B261-B264 ◽  
Author(s):  
Kamrul Islam ◽  
Rohit Chand ◽  
Dawoon Han ◽  
Ik-Soo Shin ◽  
Yong-Sang Kim
Keyword(s):  
Restriction Endonuclease ◽  
Graphene Monolayer ◽  
Endonuclease Activity ◽  
Electrochemical Assay

Determination of restriction endonuclease activity in toluene lysates of bacterial cells

1984 ◽  
Vol 97 (2) ◽  
pp. 183-185 ◽  
Author(s):  
N. N. Sokolov ◽  
A. B. Fitsner ◽  
�. B. Khoroshutina ◽  
M. Ya. Kheislere
Keyword(s):  
Restriction Endonuclease ◽  
Bacterial Cells ◽  
Endonuclease Activity

Ethanol from cellulosic biomass

1983 ◽  
Vol 300 (1100) ◽  
pp. 323-333 ◽  
Keyword(s):  
Clostridium Thermocellum ◽  
Liquid Fuel ◽  
Thermophilic Bacteria ◽  
Cellulosic Biomass ◽  
Anaerobic Microorganism ◽  
Direct Production ◽  
Effective Utilization ◽  
Mutant Strains ◽  
Total Utilization ◽  
Clostridium Thermosaccharolyticum

The major objective of this project is to achieve the direct microbiological conversion of cellulosic biomass to liquid fuel, ethanol. Within the scope of this objective, it is also the intent to maximize the conversion efficiency of ethanol production from biomass. This can be achieved through the effective utilization of both the cellulosic (six-carbon sugar) and hemicellulosic (five-carbon sugar) fractions in biomass. The degradation of cellulosic biomass is achieved through the use of a thermophilic and anaerobic bacterium, Clostridium thermocellum . This microorganism is unique in that it is able to hydrolyse both the cellulosic and hemicellulosic fractions of biomass but, unfortunately, it is not able to metabolize the pentoses. Therefore, to achieve total utilization of biomass, a second thermophilic and anaerobic microorganism, Clostridium thermosaccharolyticum , has been under study owing to its ability to convert pentoses to ethanol. Mutation, selection and adaption programmes have yielded ethanol tolerant strains of both organisms. A fermentation process using mutant strains of the anaerobic, thermophilic bacteria Clostridium thermocellum and Clostridium thermosaccharolyticum has been investigated for the direct production of ethanol from agricultural cellulosics. Through strain improvements for increased ethanol tolerance and catabolite selectivity, alcohol yields of 85% of the theoretical maximum have been obtained from solka floc with mixed culture. The method of isolation and the performance of these improved strains on both refined cellulosics and a realistic biomass, corn [maize] stover, is presented in detail.

Photo-Cross-Linked Oligonucleotide Duplex as a Decoy-DNA for Inhibition of Restriction Endonuclease Activity

2001 ◽  
Vol 29 (4) ◽  
pp. 223-233 ◽  
Author(s):  
Akira Murakami ◽  
Yoko Yamamoto ◽  
Masataka Namba ◽  
Reiko Iwase ◽  
Tetsuji Yamaoka
Keyword(s):  
Restriction Endonuclease ◽  
Endonuclease Activity ◽  
Oligonucleotide Duplex

scholarly journals Restriction endonuclease activity induced by NC-1A virus infection of aChlorella-like green alga

1986 ◽  
Vol 14 (15) ◽  
pp. 6017-6030 ◽  
Author(s):  
Yunnan Xia ◽  
Dwight E. Burbank ◽  
James L. Van Etten
Keyword(s):  
Virus Infection ◽  
Restriction Endonuclease ◽  
Green Alga ◽  
Endonuclease Activity
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