Dithiol- and NAD-dependent degradation of epoxyalkanes by Xanthobacter Py2

1995 ◽  
Vol 42 (5) ◽  
pp. 775-781 ◽  
Author(s):  
C. A. G. M. Weijers ◽  
H. Jongejan ◽  
M. C. R. Franssen ◽  
A. de Groot ◽  
J. A. M. de Bont
Keyword(s):  
Xanthobacter Py2

Cloning and expression of the genes encoding the propene monooxygenase from Xanthobacter, Py2

1996 ◽  
Vol 44 (5) ◽  
pp. 582-588 ◽  
Author(s):  
N.-Y. Zhou ◽  
C. K. Chan ◽  
Kwo Chion ◽  
D. J. Leak
Keyword(s):  
Cloning And Expression ◽  
Genes Encoding ◽  
Xanthobacter Py2

Dithiol- and NAD-dependent degradation of epoxyalkanes by Xanthobacter Py2

1995 ◽  
Vol 42 (5) ◽  
pp. 775-781 ◽  
Author(s):  
C. A. G. M. Weijers ◽  
H. Jongejan ◽  
M. C. R. Franssen ◽  
A. de Groot ◽  
J. A. M. de Bont
Keyword(s):  
Xanthobacter Py2

Chiral resolution of 2,3-epoxyalkanes by Xanthobacter Py2

1988 ◽  
Vol 27 (4) ◽  
pp. 337-340 ◽  
Author(s):  
C. A. G. M. Weijers ◽  
A. de Haan ◽  
J. A. M. de Bont
Keyword(s):  
Chiral Resolution ◽  
Xanthobacter Py2

Initial Characterization of the Enzyme and Cloning of Genes Involved in the Enantioselective Epoxyalkane Degradation by Xanthobacter PY2

Biocatalysis ◽  
1994 ◽  
Vol 10 (1-4) ◽  
pp. 227-232
Author(s):  
J. Swaving ◽  
C. A. G. M. Weijers ◽  
C. K. Chan Kwo Chion ◽  
D. J. Leak ◽  
A. J. J. Van Ooyen ◽  
...  
Keyword(s):  
Initial Characterization ◽  
Xanthobacter Py2

scholarly journals Complementation of Xanthobacter Py2 mutants defective in epoxyalkane degradation, and expression and nucleotide sequence of the complementing DNA fragment

Microbiology ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 477-484 ◽  
Author(s):  
J. Swaving ◽  
C. A. G. M. Weijers ◽  
A. J. J. v. Ooyen ◽  
J. A. M. d. Bont
Keyword(s):  
Nucleotide Sequence ◽  
Dna Fragment ◽  
Xanthobacter Py2

Batch cultivation of Xanthobacter Py2 on 1-pentene

1994 ◽  
Vol 16 (9) ◽  
pp. 989-994 ◽  
Author(s):  
F. Van Keulen ◽  
M. M. R. Da Fonseca
Keyword(s):  
Batch Cultivation ◽  
Xanthobacter Py2

scholarly journals Purification and characterization of two components of epoxypropane isomerase/carboxylase from Xanthobacter Py2

1996 ◽  
Vol 319 (2) ◽  
pp. 499-506 ◽  
Author(s):  
Chan K. N. CHION CHAN KWO ◽  
David J LEAK
Keyword(s):  
Amino Acid ◽  
Reductase Activity ◽  
Sequence Similarity ◽  
Exchange Chromatography ◽  
Amino Acid Sequences ◽  
Predicted Amino Acid Sequence ◽  
Reading Frame ◽  
C Terminus ◽  
A Subunit ◽  
Xanthobacter Py2

Epoxypropane isomerase from Xanthobacter Py2 has been resolved into at least two components (A and B) by ion-exchange chromatography. Both components were required for the degradation of epoxypropane and were purified further. Component A was apparently homohexameric with a subunit Mr of about 44000, and possessed NAD+-dependent dihydrolipoamide dehydrogenase activity and lipoamide reductase activity. It was sensitive to inhibition by o-phenanthroline and the thiol-specific reagents N-ethylmaleimide (NEM) and p-chloromercuribenzoate. Component B was homodimeric with a subunit Mr of 62170 and contained 2 mol·mol-1 FAD. It had an NADPH-dependent lipoamide reductase activity which was sensitive to NEM and p-chloromercuribenzoate. The N-terminal amino acid sequences and monomer sizes of components A and B correspond to those of ORF1 and ORF3 respectively (ORF = open reading frame) of a recently published sequence of a clone which complements mutants unable to degrade epoxypropane. NADPH was found to replace the need for a low-Mr fraction in epoxypropane degradation assays containing components A and B and NAD+. The predicted amino acid sequence of component A (ORF1) has been analysed and shown to contain a potential ADP binding site near the N-terminus and a putative cofactor binding domain near the C-terminus, with sequence similarity to the biotinyl and lipoyl binding domains of biotin-dependent carboxylases and 2-oxoacid dehydrogenases respectively. A reaction mechanism for epoxypropane degradation, incorporating recent evidence for combined isomerization and carboxylation to acetoacetate, is discussed.

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