Analysis of the respiro-fermentative growth of Saccharomyces cerevisiae on glucose in a fed-batch fermentation strategy for accurate parameter estimation

1994 ◽  
Vol 41 (6) ◽  
pp. 664-669
Author(s):  
A. O. Ejiofor ◽  
B. O. Solomon ◽  
C. Posten ◽  
W.-D. Deckwer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Parameter Estimation ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Fermentative Growth ◽  
Fermentation Strategy

Study on Process of Xylanase by Fed-Batch Fermentation

2015 ◽  
Vol 737 ◽  
pp. 381-387 ◽  
Author(s):  
Mei Zhen Gong ◽  
Rui Huang ◽  
Jin Ling Guo ◽  
Hua Shun Yu ◽  
Juan Yao ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Xylanase Activity ◽  
Fed Batch ◽  
Xylanase Production ◽  
Fermentation Kinetics ◽  
Protein Mass ◽  
Pulp Industry ◽  
Fed Batch Fermentation ◽  
Key Enzyme ◽  
Fermentation Strategy

Xylanase has good application prospect in the paper and pulp industry, feed industry, health care and bioenergy industry,etc.The xylanase is one of the key enzyme in the utilization of biomass. Fermentation kinetics equation was constructed based on Logistic and Luedeking-Piret equation. The results showed that the dissolved oxygen was related to the stirring speed, raising to 45% naturally after the log growth phase (about 30h) on the base of the optimum medium process of xylanase production by Trichoderma reesei. Fed-batch fermentation strategy was designed based on fermentation kinetics equations. The xylanase activity was further enhanced to 2406.175U/mL, enzyme activity per unit protein mass was 6573U/mg.

scholarly journals Efficient Alcoholic Conversion of Glycerol by Engineered Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Takashi Watanabe ◽  
Sadat M. R. Khattab
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nicotinamide Adenine Dinucleotide ◽  
Batch Fermentation ◽  
Plant Biomass ◽  
Glycerol Oxidation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Engineered Saccharomyces Cerevisiae ◽  
Biomass Decomposition ◽  
Engineered Strain

Glycerol is an eco-friendly solvent that enhances plant biomass decomposition via glycerolysis in many pretreatment methods. Nonetheless, the lack of efficient conversion of glycerol by natural Saccharomyces cerevisiae hinders its use in these methods. Here, we have aimed to develop a complete strategy for the generation of efficient glycerol-converting yeast by modifying the oxidation of cytosolic nicotinamide adenine dinucleotide (NADH) by an O2-dependent dynamic shuttle, while abolishing both glycerol phosphorylation and biosynthesis. By following a vigorous glycerol oxidation pathway, the engineered strain increased the conversion efficiency (CE) to up to 0.49 g ethanol/g glycerol (98% of theoretical CE), with production rate > 1 g×L×h, when glycerol was supplemented in a single fed-batch fermentation in a rich medium. Furthermore, the engineered strain fermented a mixture of glycerol and glucose, producing > 86 g/L bioethanol with 92.8% CE. To our knowledge, this is the highest ever reported titer in this field. Notably, this strategy changed conventional yeast from a non-grower on minimal medium containing glycerol to a fermenting strain with productivity of 0.25-0.5 g×L×h and 84-78% CE, which converted 90% of the substrate to products. Our findings may improve the utilization of glycerol in several eco-friendly biorefinery approaches.

Sugarcane must fed-batch fermentation by Saccharomyces cerevisiae: impact of sterilized and non-sterilized sugarcane must

2019 ◽  
Vol 112 (8) ◽  
pp. 1177-1187
Author(s):  
Maria Letícia Bonatelli ◽  
Jaciane Lutz Ienczak ◽  
Carlos Alberto Labate
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation
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