Effects of phosphoenol pyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum

1994 ◽  
Vol 40 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Marcel Gubler ◽  
Sung Min Park ◽  
Mike Jetten ◽  
Gregory Stephanopoulos ◽  
Anthony J. Sinskey
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pyruvate Carboxylase ◽  
Lysine Production ◽  
Phosphoenol Pyruvate Carboxylase ◽  
Pyruvate Carboxylase Deficiency ◽  
Phosphoenol Pyruvate

scholarly journals Changes in the kinetic properties and phosphorylation state of phosphoenol pyruvate carboxylase in Zea mays leaves in reponse to light and dark

FEBS Letters ◽  
1987 ◽  
Vol 213 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Gillian A. Nimmo ◽  
Gavin A.L. McNaughton ◽  
Charles A. Fewson ◽  
Malcolm B. Wilkins ◽  
Hugh G. Nimmo
Keyword(s):  
Zea Mays ◽  
Pyruvate Carboxylase ◽  
Kinetic Properties ◽  
Phosphorylation State ◽  
Phosphoenol Pyruvate Carboxylase ◽  
Phosphoenol Pyruvate

scholarly journals Effect of Pyruvate Carboxylase Overexpression on the Physiology of Corynebacterium glutamicum

2002 ◽  
Vol 68 (11) ◽  
pp. 5422-5428 ◽  
Author(s):  
Mattheos A. G. Koffas ◽  
Gyoo Yeol Jung ◽  
Juan C. Aon ◽  
Gregory Stephanopoulos
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pyruvate Carboxylase ◽  
Carbon Sources ◽  
Acid Synthesis ◽  
Aspartate Kinase ◽  
Growth Enhancement ◽  
Lysine Production ◽  
Carboxylase Activity ◽  
Amino Acid Synthesis ◽  
Permeabilized Cells

ABSTRACT Pyruvate carboxylase was recently sequenced in Corynebacterium glutamicum and shown to play an important role of anaplerosis in the central carbon metabolism and amino acid synthesis of these bacteria. In this study we investigate the effect of the overexpression of the gene for pyruvate carboxylase (pyc) on the physiology of C. glutamicum ATCC 21253 and ATCC 21799 grown on defined media with two different carbon sources, glucose and lactate. In general, the physiological effects of pyc overexpression in Corynebacteria depend on the genetic background of the particular strain studied and are determined to a large extent by the interplay between pyruvate carboxylase and aspartate kinase activities. If the pyruvate carboxylase activity is not properly matched by the aspartate kinase activity, pyc overexpression results in growth enhancement instead of greater lysine production, despite its central role in anaplerosis and aspartic acid biosynthesis. Aspartate kinase regulation by lysine and threonine, pyruvate carboxylase inhibition by aspartate (shown in this study using permeabilized cells), as well as well-established activation of pyruvate carboxylase by lactate and acetyl coenzyme A are the key factors in determining the effect of pyc overexpression on Corynebacteria physiology.

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