Repression of fatty-acyl-CoA oxidase-encoding gene expression is not necessarily a determinant of high-level production of dicarboxylic acids in industrial dicarboxylic-acid-producing Candida tropicalis

2001 ◽  
Vol 56 (3-4) ◽  
pp. 478-485 ◽  
Author(s):  
A. Hara ◽  
M. Ueda ◽  
T. Matsui ◽  
M. Arie ◽  
H. Saeki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dicarboxylic Acid ◽  
Candida Tropicalis ◽  
Dicarboxylic Acids ◽  
Fatty Acyl ◽  
Acyl Coa Oxidase ◽  
Encoding Gene ◽  
High Level ◽  
Level Production

scholarly journals ATP and NADPH engineering of Escherichia coli to improve the production of 4-hydroxyphenylacetic acid using CRISPRi

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yu-Ping Shen ◽  
Yu-Ling Liao ◽  
Qian Lu ◽  
Xin He ◽  
Zhi-Bo Yan ◽  
...  
Keyword(s):  
Raw Material ◽  
E Coli ◽  
Hydroxyphenylacetic Acid ◽  
Short Palindromic Repeat ◽  
Final Strain ◽  
Encoding Gene ◽  
Novel Strategy ◽  
High Level ◽  
Encoding Genes ◽  
Level Production

Abstract Background 4-Hydroxyphenylacetic acid (4HPAA) is an important raw material for the synthesis of drugs, pesticides and biochemicals. Microbial biotechnology would be an attractive approach for 4HPAA production, and cofactors play an important role in biosynthesis. Results We developed a novel strategy called cofactor engineering based on clustered regularly interspaced short palindromic repeat interference (CRISPRi) screening (CECRiS) for improving NADPH and/or ATP availability, enhancing the production of 4HPAA. All NADPH-consuming and ATP-consuming enzyme-encoding genes of E. coli were repressed through CRISPRi. After CRISPRi screening, 6 NADPH-consuming and 19 ATP-consuming enzyme-encoding genes were identified. The deletion of the NADPH-consuming enzyme-encoding gene yahK and the ATP-consuming enzyme-encoding gene fecE increased the production of 4HPAA from 6.32 to 7.76 g/L. Automatically downregulating the expression of the pabA gene using the Esa-PesaS quorum-sensing-repressing system further improved the production of 4HPAA. The final strain E. coli 4HPAA-∆yfp produced 28.57 g/L of 4HPAA with a yield of 27.64% (mol/mol) in 2-L bioreactor fed-batch fermentations. The titer and yield are the highest values to date. Conclusion This CECRiS strategy will be useful in engineering microorganisms for the high-level production of bioproducts.

IR and 13C, 17O, and 119Sn NMR spectra of some bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids

1991 ◽  
Vol 56 (9) ◽  
pp. 1908-1915 ◽  
Author(s):  
Jaroslav Holeček ◽  
Antonín Lyčka ◽  
Milan Nádvorník ◽  
Karel Handlíř
Keyword(s):  
Infrared Spectroscopy ◽  
Nmr Spectroscopy ◽  
Dicarboxylic Acid ◽  
Nmr Spectra ◽  
Dicarboxylic Acids ◽  
119Sn Nmr ◽  
Carboxylic Groups ◽  
Cyclic Oligomers ◽  
The Media ◽  
Oxygen Atoms

Infrared spectroscopy and multinuclear (13C, 17O, and 119Sn NMR spectroscopy have been used to study the structure of bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids (1-C4H9)2. Sn(X(COO)2), where X = (CH2)n (n = 0-8), CH=CH (cis and trans) and C6H4 (ortho and para).The crystalline compounds are formed by linear or cyclic oligomers or polymers whose basic building units represent a grouping composed of the central tin atom substituted by two 1-butyl groups and coordinated with both oxygen atoms of two anisobidentate carboxylic groups derived from different molecules of a dicarboxylic acid. The environment of the tin atom has a shape of a trapezoidal bipyramid. When dissolvet in non-coordinating solvents, the compounds retain the oligomeric character with unchanged structure of environment of the central tin atom. In the media of coordinating solvents the bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids form complexes whose central hexacoordinated tin atom binds two molecules of the solvent trough their donor atoms. Carboxylic groups form monodenate linkages in these complexes.

Protein body-inducing fusions for high-level production and purification of recombinant proteins in plants

2011 ◽  
Vol 9 (4) ◽  
pp. 419-433 ◽  
Author(s):  
Andrew J. Conley ◽  
Jussi J. Joensuu ◽  
Alex Richman ◽  
Rima Menassa
Keyword(s):  
Recombinant Proteins ◽  
Protein Body ◽  
High Level ◽  
Level Production

scholarly journals Identification and purification of a peroxisomal branched chain fatty acyl-CoA oxidase.

1991 ◽  
Vol 266 (36) ◽  
pp. 24676-24683
Author(s):  
P.P. Van Veldhoven ◽  
G. Vanhove ◽  
F. Vanhoutte ◽  
G. Dacremont ◽  
G. Parmentier ◽  
...  
Keyword(s):  
Fatty Acyl ◽  
Acyl Coa Oxidase ◽  
Branched Chain
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