Enhanced uridine diphosphate N-acetylglucosamine production using whole-cell catalysis

2009 ◽  
Vol 84 (4) ◽  
pp. 677-683 ◽  
Author(s):  
Hanjie Ying ◽  
Xiaochun Chen ◽  
Haiping Cao ◽  
Jian Xiong ◽  
Yuan Hong ◽  
...  
Keyword(s):  
Uridine Diphosphate ◽  
Whole Cell ◽  
Whole Cell Catalysis

GM3 trisaccharide biosynthesis and process optimization using engineered E. coli lysate and whole-cell catalysis

2020 ◽  
Vol 39 (5-6) ◽  
pp. 217-231
Author(s):  
Lipeng Feng ◽  
Jie Shi ◽  
Haofei Hong ◽  
Zhifang Zhou ◽  
Zhimeng Wu
Keyword(s):  
Process Optimization ◽  
Whole Cell ◽  
E Coli ◽  
Whole Cell Catalysis

Living whole-cell catalysis in compartmentalized emulsion

2020 ◽  
Vol 295 ◽  
pp. 122221 ◽  
Author(s):  
Qingcai Zhao ◽  
Marion B. Ansorge-Schumacher ◽  
Rainer Haag ◽  
Changzhu Wu
Keyword(s):  
Whole Cell ◽  
Whole Cell Catalysis

Biosynthesis of 1,4-butanediol from erythritol using whole-cell catalysis

2018 ◽  
Vol 37 (2) ◽  
pp. 92-96 ◽  
Author(s):  
Lu Dai ◽  
Cui Tai ◽  
Yaling Shen ◽  
Yali Guo ◽  
Fei Tao
Keyword(s):  
Whole Cell ◽  
Whole Cell Catalysis

scholarly journals Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xia Hua ◽  
Xin Zhou ◽  
GenLai Du ◽  
Yong Xu
Keyword(s):  
Oxygen Supply ◽  
Chemical Engineering ◽  
Gluconobacter Oxydans ◽  
Critical Issue ◽  
High Density ◽  
Chemical Production ◽  
Oxygen Utilization ◽  
Whole Cell ◽  
Xylonic Acid ◽  
Whole Cell Catalysis

Abstract Background The critical issue in the competitiveness between bioengineering and chemical engineering is the products titer and the volume productivity. The most direct and effective approach usually employs high-density biocatalyst, while the weakened mass transfer and evoked foam problem accompany ultrahigh-density biocatalyst loading and substrate/product titer. In high-density obligate aerobic bioconversion, oxygen as electron acceptor is a speed-limiting step in bioprocesses, but sufficient oxygen supply will lead to the foaming which results in a significant reduction in oxygen utilization and the use of additional defoamers. In this study, we designed a novel sealed-oxygen supply (SOS) biotechnology to resolve the formidable barrier of oxygen transferring rate (OTR), for bio-based fuels and chemical production process. Results Based on systemic analysis of whole-cell catalysis in Gluconobacter oxydans, a novel sealed-oxygen supply technology was smartly designed and experimentally performed for biocatalytic oxidation of alcohols, sugars and so on. By a simple operation skill of automatic online supply of oxygen in a sealed stirring tank bioreactor of SOS, OTR barrier and foaming problem was resolved with great ease. We finally obtained ultrahigh-titer products of xylonic acid (XA), 3-hydroxypropionic acid (3-HPA), and erythrulose at 588.4 g/L, 69.4 g/L, and 364.7 g/L, respectively. Moreover, the volume productivity of three chemical products was improved by 150–250% compared with normal biotechnology. This SOS technology provides a promising approach to promote bioengineering competitiveness and advantages over chemical engineering. Conclusion SOS technology was demonstrated as an economic and universally applicable approach to bio-based fuels and chemicals production by whole-cell catalysis. The novel technology greatly promotes the competitiveness of bioengineering for chemical engineering, and provides a promising platform for the green and environmental use of biofuels.

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