Polymorphisms of the human IFNG gene noncoding regions

2000 ◽  
Vol 51 (1) ◽  
pp. 50-58 ◽  
Author(s):  
J. H. Bream ◽  
M. Carrington ◽  
S. O'Toole ◽  
M. Dean ◽  
B. Gerrard ◽  
...  
Keyword(s):  
Noncoding Regions ◽  
Ifng Gene

scholarly journals The Evolution of Isochores: Evidence From SNP Frequency Distributions

Genetics ◽  
2002 ◽  
Vol 162 (4) ◽  
pp. 1805-1810 ◽  
Author(s):  
Martin J Lercher ◽  
Nick G C Smith ◽  
Adam Eyre-Walker ◽  
Laurence D Hurst
Keyword(s):  
Population Genetics ◽  
Large Scale ◽  
Gc Content ◽  
Nucleotide Composition ◽  
Compositional Variation ◽  
Mutation Bias ◽  
Single Nucleotide ◽  
Frequency Distributions ◽  
Noncoding Regions ◽  
Standard Population

AbstractThe large-scale systematic variation in nucleotide composition along mammalian and avian genomes has been a focus of the debate between neutralist and selectionist views of molecular evolution. Here we test whether the compositional variation is due to mutation bias using two new tests, which do not assume compositional equilibrium. In the first test we assume a standard population genetics model, but in the second we make no assumptions about the underlying population genetics. We apply the tests to single-nucleotide polymorphism data from noncoding regions of the human genome. Both models of neutral mutation bias fit the frequency distributions of SNPs segregating in low- and medium-GC-content regions of the genome adequately, although both suggest compositional nonequilibrium. However, neither model fits the frequency distribution of SNPs from the high-GC-content regions. In contrast, a simple population genetics model that incorporates selection or biased gene conversion cannot be rejected. The results suggest that mutation biases are not solely responsible for the compositional biases found in noncoding regions.

Levels of ordering in coding and noncoding regions of DNA sequences

1996 ◽  
Vol 222 (5) ◽  
pp. 354-360 ◽  
Author(s):  
V.R. Chechetkin ◽  
V.V. Lobzin
Keyword(s):  
Dna Sequences ◽  
Noncoding Regions

Replication of chimeric tobacco mosaic viruses which carry heterologous combinations of replicase genes and 3′ noncoding regions

Virology ◽  
1988 ◽  
Vol 164 (1) ◽  
pp. 290-293 ◽  
Author(s):  
Masayuki Ishikawa ◽  
Tetsuo Meshi ◽  
Yuichiro Watanabe ◽  
Yoshimi Okada
Keyword(s):  
Noncoding Regions

The chicken ubiquitin gene contains a heat shock promoter and expresses an unstable mRNA in heat-shocked cells

1986 ◽  
Vol 6 (12) ◽  
pp. 4602-4610
Author(s):  
U Bond ◽  
M J Schlesinger
Keyword(s):  
Heat Shock ◽  
Chicken Embryo ◽  
Actinomycin D ◽  
Genomic Library ◽  
Protein Coding ◽  
Noncoding Regions ◽  
Genomic Location ◽  
Embryo Fibroblasts ◽  
The Stability ◽  
Chicken Embryo Fibroblasts

A chicken genomic library was screened to obtain genomic clones for ubiquitin genes. Two genes that differ in their genomic location and organization were identified. One gene, designated Ub I, contains four copies of the protein-coding sequence arranged in tandem, while the second gene, Ub II, contains three. The origin of the two major mRNAs that are induced after heat shock in chicken embryo fibroblasts was determined by generating DNA probes from the 5'-and 3'-noncoding regions of the two genes. Both mRNAs are transcribed from Ub I, the larger being the unspliced precursor of the smaller. A 674-base-pair intron was located within the 5'-noncoding region of Ub I. The second gene, Ub II, does not appear to code for an RNA species in normal or heat-shocked chicken embryo fibroblasts. The expression of ubiquitin mRNA during heat shock and recovery was examined. Addition of actinomycin D before heat shock completely abolished the response of ubiquitin mRNA to the stress. Analysis of the stability of the mRNA during recovery revealed that the mRNA accumulated during the heat shock is rapidly degraded with a half-life of approximately 1.5 h, suggesting a specialized but transient role for ubiquitin during heat shock.

Regulation, linkage, and sequence of mouse metallothionein I and II genes

1984 ◽  
Vol 4 (7) ◽  
pp. 1221-1230
Author(s):  
P F Searle ◽  
B L Davison ◽  
G W Stuart ◽  
T M Wilkie ◽  
G Norstedt ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Hela Cells ◽  
Conserved Sequences ◽  
Noncoding Regions ◽  
Coding Regions ◽  
Four Blocks ◽  
I Gene

The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other mammals) reveals that the coding regions are highly conserved even at "silent" positions but that the noncoding regions and introns are extremely divergent between primates and rodents. There are four blocks of conserved sequences in the promoters of mouse MT-I, mouse MT-II, and human MT-IIA genes; one includes the TATAAA sequence, and another has been implicated in regulation by heavy metals. Mouse MT-I and MT-II mRNAs are induced to approximately the same extent in vivo in response to cadmium, dexamethasone, or lipopolysaccharide. Mouse MT-I and MT-II genes are regulated by metals but not by glucocorticoids after transfection into HeLa cells.

Sign in / Sign up

Export Citation Format

Share Document