Localization and Identification of Populations of Phosphatase-Active Bacterial Cells Associated with Activated Sludge Flocs

1999 ◽  
Vol 38 (3) ◽  
pp. 201-214 ◽  
Author(s):  
F. Van Ommen Kloeke
Keyword(s):  
Activated Sludge ◽  
Bacterial Cells ◽  
Sludge Flocs

scholarly journals Molecular characterization of filamentous bacteria isolated from full-scale activated sludge processes

2007 ◽  
Author(s):  
◽  
Zinhle Marrengane
Keyword(s):  
Activated Sludge ◽  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Support Network ◽  
Bulk Liquid ◽  
Bacterial Cells ◽  
Filamentous Bacteria ◽  
Vast Number ◽  
Sludge Flocs ◽  
Activated Sludge Processes

Activated sludge flocs are responsible for flocculation, settling and dewaterability. It is important to maintain the growth off loc-forming bacteria for efficient sludge settleability and compaction for good quality effluent. Filamentous bacteria on the other hand are believed to provide rigid support network or backbone upon which floc-forming bacteria adhere to form stable activated sludge flocs (Wilderer et al., 2002; Ramothokang et al., 2003). Filamentous bacteria can also be detrimental to the process when they outgrow floc-forming bacteria. Morphologically filamentous bacteria are at an advantage as they have higher outward growth velocity and can extend freely to bulk liquid substrate. Proliferation of filamentous bacteria causes foaming and bulking (Martins et al., 2004). Although chemical alleviation measures to circumvent bulking are present, they are symptomatic (Chang et al., 2004). Eikelboom (1975) developed the first identification keys for the classification of filamentous bacteria that is primarily based on morphological characteristics and microscopic examination. Although very useful, this type of identification has its limitations. For instance some filamentous bacteria can change morphology in response to changes in the environment and although some of them can be morphologically similar they may vary considerably in their physiology and taxonomy (Martins et al., 2004). A vast number of filamentous bacteria are still very poorly understood which could be due to the problems of cultivation due to their slow growing nature and maintenance of cultures (Rossetti et al., 2006). This limitation necessitates a molecular approach to resolve the taxonomy of filamentous bacteria as it is a culture-independent technique which is highly accurate. This project was undertaken to verify the identity of pure cultures of filamentous bacteria isolated previously through the application of molecular techniques. The 16S rDNA are conserved regions in bacterial cells and they can be extracted and specific nucleic acid fragments amplified. Denaturation gradient gel electrophoresis enabled the separation of fragments of identical length but different size and served as an indication of purity (Muyzer et al., 1993).

Adsorption of bacterial cells onto activated sludge flocs

1999 ◽  
Vol 87 (4) ◽  
pp. 513-518 ◽  
Author(s):  
Satoshi Soda ◽  
Michihiko Ike ◽  
Masanori Fujita
Keyword(s):  
Activated Sludge ◽  
Bacterial Cells ◽  
Sludge Flocs

Application of Image Analysis System for Study of Activated Sludge Flocs

1986 ◽  
Vol 21 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Da-hong Li ◽  
J. J. Ganczarczyk
Keyword(s):  
Image Analysis ◽  
Activated Sludge ◽  
Statistical Processing ◽  
Equivalent Diameter ◽  
Image Analysis System ◽  
Sectional Area ◽  
Cross Sectional ◽  
Computerized Image Analysis ◽  
Sludge Flocs ◽  
Analysis System

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.

scholarly journals Deep learning-based morphology classification of activated sludge flocs in wastewater treatment plants

2021 ◽  
Author(s):  
Hisashi Satoh ◽  
Yukari Kashimoto ◽  
Naoki Takahashi ◽  
Takashi Tsujimura
Keyword(s):  
Wastewater Treatment ◽  
Deep Learning ◽  
Activated Sludge ◽  
Morphological Change ◽  
Wastewater Treatment Plants ◽  
Filamentous Bacteria ◽  
Sludge Flocs

A deep learning-based two-label classifier 1 recognized a 20% morphological change in the activated flocs. Classifier-2 quantitatively recognized an abundance of filamentous bacteria in activated flocs.

Study of factors affecting simultaneous nitrification and denitrification (SND)

1999 ◽  
Vol 39 (6) ◽  
pp. 61-68 ◽  
Author(s):  
Klangduen Pochana ◽  
Jürg Keller
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Physical Phenomenon ◽  
Treatment Plant ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Factors Affecting ◽  
Floc Size ◽  
Sludge Flocs ◽  
Activated Sludge Systems

Experiments have been performed to gain an understanding of the conditions and processes governing the occurrence of SND in activated sludge systems. Sequencing batch reactors (SBRs) have been operated under controlled conditions using the wastewater from the first anaerobic pond in an abattoir wastewater treatment plant. Under specific circumstances, up to 95% of total nitrogen removal through SND has been found in the system. Carbon source and oxygen concentrations were found to be important process parameters. The addition of acetate as an external carbon source resulted in a significant increase of SND activity in the system. Stepwise change of DO concentration has also been observed in this study. Experiments to determine the effect of the floc size on SND have been performed in order to test the hypothesis that SND is a physical phenomenon, governed by the diffusion of oxygen into the activated sludge flocs. Initial results support this hypothesis but further experimental confirmation is still required.

Effects of potassium ion on sludge settling, dewatering and effluent properties

1998 ◽  
Vol 37 (4-5) ◽  
pp. 317-324 ◽  
Author(s):  
Sudhir N. Murthy ◽  
John T. Novak
Keyword(s):  
Activated Sludge ◽  
Field Studies ◽  
Potassium Ion ◽  
Potassium Ions ◽  
Nutrient Requirements ◽  
Sludge Dewatering ◽  
Capillary Suction ◽  
Sludge Flocs ◽  
Sludge Properties ◽  
Sludge Settling

Potassium ions appear to play an important role in determining the nature of activated sludge flocs. Relative to sodium, the concentration of potassium ions in most industrial activated sludge is typically low. Laboratory and field studies were conducted to examine the influence of potassium on activated sludge properties. The concentration of potassium affected the concentration of readily extractable (slime) proteins in the floc and the proteins in the surrounding solution. In laboratory tests, an increase in this cation's concentration beyond nutrient requirements impeded sludge dewatering properties as measured by capillary suction time (CST) and specific resistance to filtration (SRF) and associated with an increase in soluble protein. An increase in effluent total organic carbon and effluent turbidity was observed at higher concentrations of this ion. Conversely, an increase in concentration of potassium ion improved the settling properties of sludge with low equivalent monovalent to divalent cation ratio.

scholarly journals Efficiency of Biological Phosphorus Removal by Filamentous Bacteria

2016 ◽  
Vol 21 (1-2) ◽  
pp. 117-123 ◽  
Author(s):  
Alicja Machnicka ◽  
Klaudiusz Grübel
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Water Environment ◽  
Treatment Plant ◽  
Chemical Precipitation ◽  
Heterogeneous Structure ◽  
Filamentous Bacteria ◽  
Biological Phosphorus Removal ◽  
High Concentration ◽  
Sludge Flocs

AbstractPhosphorus removal in wastewater treatment plant is carried out by chemical precipitation, advanced biological treatment or a combination of both. One of the biggest problems with high concentration of phosphorus in water environment is eutrophication. Activated sludge flocs have a heterogeneous structure, which consist of a variety of microorganisms. Filamentous bacteria are normally present in the activated sludge and have ability to assimilation of phosphorus. In this study phosphorus accumulation by isolated filamentous bacteria from activated sludge foam was present.

Flotation efficiency of activated sludge flocs using population balance model in dissolved air flotation

2006 ◽  
Vol 23 (2) ◽  
pp. 271-278 ◽  
Author(s):  
Heung-Joe Jung ◽  
Jae-Wook Lee ◽  
Do-Young Choi ◽  
Seong-Jin Kim ◽  
Dong-Heui Kwak
Keyword(s):  
Activated Sludge ◽  
Population Balance ◽  
Balance Model ◽  
Population Balance Model ◽  
Dissolved Air Flotation ◽  
Sludge Flocs ◽  
Air Flotation ◽  
Dissolved Air

Strength and breakage of activated sludge flocs

2010 ◽  
Vol 199 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Yuan Yuan ◽  
Ramin R. Farnood
Keyword(s):  
Activated Sludge ◽  
Sludge Flocs
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