Immunocytochemical distribution of nitric oxide synthase in the human corpus cavernosum: an electron microscopical study using the tyramide signal amplification technique

2001 ◽  
Vol 29 (3) ◽  
pp. 168-172 ◽  
Author(s):  
Andreas Stanarius ◽  
Stefan Ückert ◽  
Stefan A. Machtens ◽  
Christian G. Stief ◽  
Gerald Wolf ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Signal Amplification ◽  
Corpus Cavernosum ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
Tyramide Signal Amplification ◽  
Amplification Technique ◽  
Electron Microscopical ◽  
Oxide Synthase

Immunocytochemical distribution of nitric oxide synthase in the human seminal vesicle: a light and electron microscopical study

2003 ◽  
Vol 31 (4) ◽  
pp. 262-266 ◽  
Author(s):  
Stefan �ckert ◽  
Andreas Stanarius ◽  
Christian G. Stief ◽  
Gerald Wolf ◽  
Udo Jonas ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Seminal Vesicle ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
Electron Microscopical ◽  
Oxide Synthase

scholarly journals Relaxation Effect of Patchouli Alcohol in Rat Corpus Cavernous and Its Underlying Mechanisms

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Fangjun Chen ◽  
Yifei Xu ◽  
Jing Wang ◽  
Xufeng Yang ◽  
Hongying Cao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Soluble Guanylate Cyclase ◽  
Corpus Cavernosum ◽  
Relaxation Effect ◽  
Calcium Antagonism ◽  
Nitric Oxide Signaling ◽  
Patchouli Alcohol ◽  
Cox Inhibitor ◽  
Oxide Synthase

In this study, we investigated the relaxation effect and mechanisms of patchouli alcohol (PA) on rat corpus cavernosum. Corpus cavernosum strips were used in organ baths for isometric tension studies. The results showed that PA demonstrated concentration-dependent relaxation effect on rat corpus cavernosum. The relaxant response to PA was not influenced by tetrodotoxin and atropine while it was significantly inhibited by removal of endothelium. L-NG-nitroarginine methyl ester (L-NAME, a nitric oxide synthase inhibitor) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, a soluble guanylate cyclase inhibitor) significantly inhibited relaxation response to PA, whereas indomethacin (COX inhibitor) had no effect on PA-induced relaxation. The treatment of endothelium-deprived corpus cavernosum with several potassium channel blockers including tetraethylammonium (TEA), 4-aminopyridine (4-AP), and glibenclamide had no effect on PA-induced relaxation. Endothelium-deprived corpus cavernosal contractions induced by cumulative addition of Ca2+ to high KCl solution without CaCl2 were significantly inhibited by PA. Also, PA improved relaxant capacity of sildenafil in rat corpus cavernosum. In addition, the perfusion with PA significantly increased the levels of cGMP and expression of mRNA and protein of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS). Furthermore, intracavernous injection of PA enhanced the rise in intracavernous pressure in rats during cavernosal nerve electric stimulation. In conclusion, PA relaxed the rat corpus cavernosum attributed to both endothelium-dependent and -independent properties. While the former component was mostly involved in nitric oxide signaling pathway, the endothelium-independent mechanism involved in PA-induced relaxation was probably linked to calcium antagonism.

IMMUNOCYTOCHEMICAL DISTRIBUTION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE (eNOS) IN HUMAN CORPUS CAVERNOSUM

1999 ◽  
pp. 221 ◽  
Author(s):  
Andreas Stanarius ◽  
Stefan Uckert ◽  
Stefan A. Machtens ◽  
Christian G. Stief ◽  
Gerald Wolf ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Corpus Cavernosum ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Effect of berberine on the mRNA expression of nitric oxide synthase (NOS) in rat corpus cavernosum

2005 ◽  
Vol 25 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Tan Yan ◽  
Ming Zhangyin ◽  
Tang Qiang ◽  
Jiang Zhaojian ◽  
Hu Benrong ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Mrna Expression ◽  
Corpus Cavernosum ◽  
Oxide Synthase
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