Comparison of the synergistic effects of tamsulosin versus phentolamine on penile erection: in vitro and in vivo studies

1999 ◽  
Vol 27 (6) ◽  
pp. 437-444 ◽  
Author(s):  
S. C. Kim ◽  
K. K. Seo ◽  
S. K. Lee ◽  
E. S. Song ◽  
M. Y. Lee
Keyword(s):  
Penile Erection ◽  
Synergistic Effects ◽  
In Vivo Studies

scholarly journals Nutraceutical Activity in Osteoarthritis Biology: A Focus on the Nutrigenomic Role

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1232
Author(s):  
Stefania D’Adamo ◽  
Silvia Cetrullo ◽  
Veronica Panichi ◽  
Erminia Mariani ◽  
Flavio Flamigni ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Antioxidant Activities ◽  
Synergistic Effects ◽  
National Health System ◽  
Bioactive Molecules ◽  
Joint Disease ◽  
In Vivo Studies ◽  
Wide Range

Osteoarthritis (OA) is a disease associated to age or conditions that precipitate aging of articular cartilage, a post-mitotic tissue that remains functional until the failure of major homeostatic mechanisms. OA severely impacts the national health system costs and patients’ quality of life because of pain and disability. It is a whole-joint disease sustained by inflammatory and oxidative signaling pathways and marked epigenetic changes responsible for catabolism of the cartilage extracellular matrix. OA usually progresses until its severity requires joint arthroplasty. To delay this progression and to improve symptoms, a wide range of naturally derived compounds have been proposed and are summarized in this review. Preclinical in vitro and in vivo studies have provided proof of principle that many of these nutraceuticals are able to exert pleiotropic and synergistic effects and effectively counteract OA pathogenesis by exerting both anti-inflammatory and antioxidant activities and by tuning major OA-related signaling pathways. The latter are the basis for the nutrigenomic role played by some of these compounds, given the marked changes in the transcriptome, miRNome, and methylome. Ongoing and future clinical trials will hopefully confirm the disease-modifying ability of these bioactive molecules in OA patients.

scholarly journals Low Intensity Pulsed Ultrasound for Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1488
Author(s):  
Colleen McCarthy ◽  
Gulden Camci-Unal
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Synergistic Effects ◽  
In Vivo Studies ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

As explained by Wolff’s law and the mechanostat hypothesis, mechanical stimulation can be used to promote bone formation. Low intensity pulsed ultrasound (LIPUS) is a source of mechanical stimulation that can activate the integrin/phosphatidylinositol 3-OH kinase/Akt pathway and upregulate osteogenic proteins through the production of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). This paper analyzes the results of in vitro and in vivo studies that have evaluated the effects of LIPUS on cell behavior within three-dimensional (3D) titanium, ceramic, and hydrogel scaffolds. We focus specifically on cell morphology and attachment, cell proliferation and viability, osteogenic differentiation, mineralization, bone volume, and osseointegration. As shown by upregulated levels of alkaline phosphatase and osteocalcin, increased mineral deposition, improved cell ingrowth, greater scaffold pore occupancy by bone tissue, and superior vascularization, LIPUS generally has a positive effect and promotes bone formation within engineered scaffolds. Additionally, LIPUS can have synergistic effects by producing the piezoelectric effect and enhancing the benefits of 3D hydrogel encapsulation, growth factor delivery, and scaffold modification. Additional research should be conducted to optimize the ultrasound parameters and evaluate the effects of LIPUS with other types of scaffold materials and cell types.

scholarly journals Effects of Korean ginseng berry extract (GB0710) on penile erection: evidence from in vitro and in vivo studies

2013 ◽  
Vol 15 (4) ◽  
pp. 503-507 ◽  
Author(s):  
KS Cho ◽  
CW Park ◽  
CK Kim ◽  
HY Jeon ◽  
WG Kim ◽  
...  
Keyword(s):  
Penile Erection ◽  
In Vivo Studies ◽  
Korean Ginseng

In vitro and in vivo studies on the importance of the soluble guanylyl cyclase α1 subunit in penile erection

2010 ◽  
Vol 28 (5) ◽  
pp. 643-650 ◽  
Author(s):  
Kelly Decaluwé ◽  
Sofie Nimmegeers ◽  
Robrecht Thoonen ◽  
Emanuel Buys ◽  
Peter Brouckaert ◽  
...  
Keyword(s):  
Guanylyl Cyclase ◽  
Penile Erection ◽  
Soluble Guanylyl Cyclase ◽  
In Vivo Studies ◽  
Α1 Subunit

scholarly journals Comprehensive Review of Hybrid Collagen and Silk Fibroin for Cutaneous Wound Healing

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3097 ◽  
Author(s):  
Ruth Naomi ◽  
Juthamas Ratanavaraporn ◽  
Mh Busra Fauzi
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Silk Fibroin ◽  
Synergistic Effects ◽  
In Vivo Studies ◽  
Cutaneous Wound Healing ◽  
Hybrid Scaffold ◽  
Cutaneous Wound

The use of hybridisation strategy in biomaterials technology provides a powerful synergistic effect as a functional matrix. Silk fibroin (SF) has been widely used for drug delivery, and collagen (Col) resembles the extracellular matrix (ECM). This systematic review was performed to scrutinise the outcome of hybrid Col and SF for cutaneous wound healing. This paper reviewed the progress of related research based on in vitro and in vivo studies and the influence of the physicochemical properties of the hybrid in wound healing. The results indicated the positive outcome of hybridising Col and SF for cutaneous wound healing. The hybridisation of these biomaterials exhibits an excellent moisturising property, perfectly interconnected structure, excellent water absorption and retention capacity, an acceptable range of biodegradability, and synergistic effects in cell viability. The in vitro and in vivo studies clearly showed a promising outcome in the acceleration of cutaneous wound healing using an SF and Col hybrid scaffold. The review of this study can be used to design an appropriate hybrid scaffold for cutaneous wound healing. Therefore, this systematic review recapitulated that the hybridisation of Col and SF promoted rapid cutaneous healing through immediate wound closure and reepithelisation, with no sign of adverse events. This paper concludes on the need for further investigations of the hybrid SF and Col in the future to ensure that the hybrid biomaterials are well-suited for human skin.

scholarly journals Revealing the Potential Application of EC-Synthetic Retinoid Analogues in Anticancer Therapy

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 506
Author(s):  
Mohamed R. Abdelaal ◽  
Sameh H. Soror ◽  
Mohamed R. Elnagar ◽  
Hesham Haffez
Keyword(s):  
Cell Cycle ◽  
Retinoic Acid ◽  
Cell Lines ◽  
Synergistic Effects ◽  
Substantial Reduction ◽  
Fluorescein Isothiocyanate ◽  
In Vivo Studies ◽  
Mcf 7

(1) Background and Aim: All-trans retinoic acid (ATRA) induces differentiation and inhibits growth of many cancer cells. However, resistance develops rapidly prompting the urgent need for new synthetic and potent derivatives. EC19 and EC23 are two synthetic retinoids with potent stem cell neuro-differentiation activity. Here, these compounds were screened for their in vitro antiproliferative and cytotoxic activity using an array of different cancer cell lines. (2) Methods: MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, AV/PI (annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI)), cell cycle analysis, immunocytochemistry, gene expression analysis, Western blotting, measurement of glutamate and total antioxidant concentrations were recruited. (3) Results: HepG2, Caco-2, and MCF-7 were the most sensitive cell lines; HepG2 (ATRA; 36.2, EC19; 42.2 and EC23; 0.74 µM), Caco-2 (ATRA; 58.0, EC19; 10.8 and EC23; 14.7 µM) and MCF-7 (ATRA; 99.0, EC19; 9.4 and EC23; 5.56 µM). Caco-2 cells were selected for further biochemical investigations. Isobologram analysis revealed the combined synergistic effects with 5-fluorouracil with substantial reduction in IC50. All retinoids induced apoptosis but EC19 had higher potency, with significant cell cycle arrest at subG0-G1, -S and G2/M phases, than ATRA and EC23. Moreover, EC19 reduced cellular metastasis in a transwell invasion assay due to overexpression of E-cadherin, retinoic acid-induced 2 (RAI2) and Werner (WRN) genes. (4) Conclusion: The present study suggests that EC-synthetic retinoids, particularly EC19, can be effective, alone or in combinations, for potential anticancer activity to colorectal cancer. Further in vivo studies are recommended to pave the way for clinical applications.

scholarly journals In vitro and in vivo effects of rifabutin alone or combined with atovaquone against Toxoplasma gondii.

1996 ◽  
Vol 40 (9) ◽  
pp. 2015-2020 ◽  
Author(s):  
S Romand ◽  
C Della Bruna ◽  
R Farinotti ◽  
F Derouin
Keyword(s):  
Toxoplasma Gondii ◽  
Synergistic Effects ◽  
Survival Rates ◽  
Culture Method ◽  
In Vivo Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Brain ◽  
Acute Toxoplasmosis

The efficacy of rifabutin (RIFA) alone or in combination with atovaquone (ATO) was examined in vitro and in a murine model of acute toxoplasmosis. In vitro studies were performed with MRC5 fibroblast tissue cultures, with quantification of Toxoplasma growth by enzyme-linked immunosorbent assay. For in vivo studies, mice were acutely infected with 10(4) tachyzoites of the virulent RH strain and were then treated perorally for 10 days from day 1 or day 4 postinfection. The efficacy of each drug regimen was assessed by determination of survival rates and sequential titration of parasites in blood, brain, and lungs by a tissue culture method. In vitro, RIFA was inhibitory for Toxoplasma growth at concentrations between 0.5 and 20 micrograms/ml; the 50% inhibitory concentration was estimated to be 1.68 micrograms/ml. When RIFA and ATO were combined, synergistic effects were noted for RIFA at 20 micrograms/ml combined with ATO at 0.01 or 0.02 microgram/ml and RIFA at 1, 2, or 5 micrograms/ml combined with ATO at 0.02 microgram/ml. In vivo, administration of RIFA at 200 mg/kg of body weight per day from day 1 to day 10 resulted in a 100% protection during treatment, with clearance of parasites from the blood, brain, and lungs. After the cessation of therapy, relapses occurred in the brain and lungs; the mortality was 46% at the end of the experiment (day 30). Among the mice treated with RIFA at 200 mg/kg/day from day 4 to day 14, no death was recorded during the treatment period and a marked reduction in parasite burdens was observed in blood and tissues; however, relapses occurred and 10% of mice survived until day 30. Administration of RIFA at 200 mg/kg/day in combination with ATO at 100 mg/kg/day resulted in a marked prolongation of survival compared with that for mice that received ATO or RIFA alone. However, in mice receiving the combination, parasite burdens in blood and organs were similar to those in mice treated with RIFA alone. These results confirmed the activity of RIFA in the treatment of acute toxoplasmosis and the potential of the combination of RIFA-ATO since the two drugs act synergistically against Toxoplasma gondii.

scholarly journals In vitro and in vivo studies on the importance of the soluble gyanylyl cyclase alpha 1 subunit in penile erection

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Kelly Decaluwé ◽  
Sofie Nimmegeers ◽  
Robrecht Thoonen ◽  
Peter Brouckaert ◽  
Johan Van de Voorde
Keyword(s):  
Penile Erection ◽  
In Vivo Studies
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