Inhibition by Vitamin C of DNA adduct formation and arylamine N -acetyltransferase activity in human bladder tumor cells

2000 ◽  
Vol 28 (4) ◽  
pp. 235-240 ◽  
Author(s):  
H. C. Wu ◽  
H. F. Lu ◽  
C. F. Hung ◽  
J. G. Chung
Keyword(s):  
Vitamin C ◽  
Tumor Cells ◽  
Bladder Tumor ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Human Bladder ◽  
Acetyltransferase Activity

Paeonol promotes arylamine N‐acetyltransferase activity in human bladder tumor cells

1999 ◽  
Vol 71 (1-2) ◽  
pp. 211-219
Author(s):  
Huei L. Chang ◽  
Lii T. Wu ◽  
Feng T. Yeh ◽  
Hsueh F. Lu ◽  
Chin C. Yeh ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Bladder Tumor ◽  
Human Bladder ◽  
Acetyltransferase Activity

Detection of Tumor Cells in Bladder Washings by a Monoclonal Antibody to Human Bladder Tumor-Associated Antigen

1988 ◽  
Vol 140 (3) ◽  
pp. 672-677 ◽  
Author(s):  
Chi-Wei Lin ◽  
Deborah A. Young ◽  
Sandra D. Kirley ◽  
Aii-Hwa Khaw ◽  
George R. Prout
Keyword(s):  
Monoclonal Antibody ◽  
Tumor Cells ◽  
Bladder Tumor ◽  
Human Bladder ◽  
Tumor Associated Antigen

Targeting bladder tumor cells in vivo and in the urine by a peptide identified using phage display

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 10071-10071
Author(s):  
T. Kwon ◽  
B. Lee ◽  
E. Yoo ◽  
S. Chung ◽  
Y. Park ◽  
...  
Keyword(s):  
Phage Display ◽  
Tumor Cells ◽  
Bladder Tumor ◽  
Tumor Model ◽  
Human Bladder ◽  
Selective Binding ◽  
Tumor Xenografts ◽  
Tumor Tissues ◽  
Fluorescent Peptide

10071 Background: Bladder cancer is one of the most common tumors of genitourinary tract. Selective delivery of drugs to tumor tissues is important for effective tumor therapy. Here we identified a peptide targeting bladder tumor cells using phage display. Methods: Phage peptide library containing CX7C (C-cysteine and X-any amino acid residue) was based on T7 415–1b phage vector (Novagen). Tumor xenografts were prepared by subcutaneously injecting BALB/c Nu/nu female nude mice with HT-1376 bladder tumor cells. For a carcinogen-induced tumor model, Fischer 344 female rats were supplied ad libitum with tap water containing 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) for 8 weeks. Results: A phage library containing CX7C random peptides was screened for selective binding to cells from human bladder tumor xenografts. Selected phage clones were individually evaluated for binding to cultured bladder tumor cells and for binding to cells from human tumor tissues of six patients. The peptide displayed by the most promising clone was synthesized and designated as Bld-1. Fluorescein-conjugated Bld-1 peptide showed selective binding to frozen sections of human bladder tumor tissues of three patients. In vivo tumor targeting was examined in a carcinogen-induced rat tumor model (n=20). When the fluorescent peptide was introduced into the bladder lumen, it selectively bound to tumor epithelium. Next, when the fluorescent peptide was intravenously injected into the tail vein, it homed to the bladder tumor, but was not detectable in normal bladder and control organs such as lung. Moreover, the fluorescent peptide bound to cells from urinary specimens of tumor patients (n=10), whereas little binding was observed in cells from healthy individuals (n=3). Conclusions: The Bld-1 peptide may be useful for targeting bladder tumor cells in vivo and in the urine. No significant financial relationships to disclose.

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