Concurrent and Independent HCO − 3 and Cl −; Secretion in a Human Pancreatic Duct Cell Line (CAPAN-1)

1998 ◽  
Vol 164 (2) ◽  
pp. 155-167 ◽  
Author(s):  
H.S. Cheng ◽  
P.Y. Leung ◽  
S.B. Cheng Chew ◽  
P.S. Leung ◽  
S.Y. Lam ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Cell Line ◽  
Duct Cell ◽  
Pancreatic Duct Cell ◽  
Cl Secretion

Exendin-4 differentiation of a human pancreatic duct cell line into endocrine cells: Involvement of PDX-1 and HNF3? transcription factors

2002 ◽  
Vol 192 (3) ◽  
pp. 304-314 ◽  
Author(s):  
Jie Zhou ◽  
Marco A. Pineyro ◽  
Xiaolin Wang ◽  
Maire E. Doyle ◽  
Josephine M. Egan
Keyword(s):  
Transcription Factors ◽  
Pancreatic Duct ◽  
Cell Line ◽  
Endocrine Cells ◽  
Duct Cell ◽  
Pancreatic Duct Cell

3,5,3′-Triiodo-L-thyronine enhances the differentiation of a human pancreatic duct cell line (hPANC-1) towards a β-cell-Like phenotype

2005 ◽  
Vol 204 (1) ◽  
pp. 286-296 ◽  
Author(s):  
Silvia Misiti ◽  
Emanuela Anastasi ◽  
Salvatore Sciacchitano ◽  
Cecilia Verga Falzacappa ◽  
Laura Panacchia ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Cell Line ◽  
Duct Cell ◽  
Β Cell ◽  
Pancreatic Duct Cell

Calcium-Activated Chloride Currents and Non-selective Cation Channels in a Novel Cystic Fibrosis-Derived Human Pancreatic Duct Cell Line

1996 ◽  
Vol 225 (2) ◽  
pp. 505-513 ◽  
Author(s):  
Ana Luisa Eguiguren ◽  
Juan Rı́os ◽  
Nora Riveros ◽  
Francisco V. Sepúlveda ◽  
Andrés Stutzin
Keyword(s):  
Cystic Fibrosis ◽  
Pancreatic Duct ◽  
Cell Line ◽  
Duct Cell ◽  
Cation Channels ◽  
Chloride Currents ◽  
Pancreatic Duct Cell

Immortalization of bovine pancreatic duct epithelial cells

1996 ◽  
Vol 270 (4) ◽  
pp. G676-G683 ◽  
Author(s):  
L. R. Marino ◽  
C. U. Cotton
Keyword(s):  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Duct Cell ◽  
Simian Virus ◽  
T Antigen ◽  
Intracellular Camp ◽  
Large T Antigen ◽  
Pancreatic Duct Cell

Pancreatic duct cell lines have been isolated from a number of animal and human tumors, but none appear to express ion transport properties expected for differentiated pancreatic duct epithelial cells. We sought to generate an immortalized ductal cell line from well-differentiated primary cultures of bovine pancreatic duct epithelium. Epithelial cells from the main duct of the bovine pancreas were isolated and immortalized by transfection with a DNA construct encoding simian virus 40 large T antigen. A single clone (BPD1) survived negative selection and was maintained in culture for > 100 passages over 2 yr. The cells grow readily in culture as monolayers and express several properties characteristic of differentiated pancreatic ductal epithelium. The cells do not appear to form a functional tight junction complex, since the transepithelial resistance of the monolayer cultures grown on a permeable support is < 10 omega.cm2. Northern blot analysis revealed that the cells continue to express simian virus 40 large T antigen and contain significant levels of mRNA for proteins thought to be important in transepithelial bicarbonate secretion [carbonic anhydrase II, Cl-/HCO3- exchanger, Na+/H+ exchanger, and cystic fibrosis transmembrane conductance regulator (CFTR)]. In vivo pancreatic ductal secretion is stimulated by the peptide hormone secretin. The secretin receptor is expressed and functionally coupled to adenylate cyclase in the immortalized cells, since secretin caused a dose-dependent accumulation of adenosine 3'5'-cyclic monophosphate (cAMP; approximately 20-fold increase over basal levels) with a mean effective concentration of 15 nM. Elevation of intracellular cAMP by exposure of the cells to forskolin (10 microM) or secretin (0.1 microM) increase plasma membrane Cl- permeability, most likely mediated by activation of CFTR. The results of these studies demonstrate that the pancreatic duct cell line (BPD1) retains several properties exhibited by the secretory epithelial cells that line the pancreatic ductal tree. This cell line should prove useful for studies of expression, function, and regulation of pancreatic duct cell proteins.

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