The Temperature Dependence of Intracellular pH in Isolated Frog Skeletal Muscle: Lessons Concerning the Na + -H + Exchanger

1998 ◽  
Vol 161 (3) ◽  
pp. 215-225 ◽  
Author(s):  
M. Marjanovic ◽  
A.C. Elliott ◽  
M.J. Dawson
Keyword(s):  
Skeletal Muscle ◽  
Temperature Dependence ◽  
Intracellular Ph ◽  
Frog Skeletal Muscle

Intracellular pH regulation in detubulated frog skeletal muscle fibers

1996 ◽  
Vol 271 (4) ◽  
pp. C1358-C1366 ◽  
Author(s):  
R. W. Putnam
Keyword(s):  
Skeletal Muscle ◽  
Intracellular Ph ◽  
Ph Regulation ◽  
Muscle Fibers ◽  
Disulfonic Acid ◽  
Frog Skeletal Muscle ◽  
Intracellular Ph Regulation ◽  
Tubular Membrane ◽  
Skeletal Muscle Fibers ◽  
Recovery From Acidification

Intracellular pH regulation was studied in semitendinosus muscle fibers from frog (Rana pipiens). Intracellular pH (pHi) was measured with recessed-tip glass microelectrodes and membrane potential with conventional microelectrodes. Fibers had their connections between the surface and transverse tubular membrane disrupted (detubulation) with the formamide shock technique. Fibers were approximately 80% detubulated as determined by the decrease in membrane capacitance and the loss of contractile capability. The initial rate of pHi recovery from acidification to approximately 6.8 (no CO2) was dependent on external buffering power, reaching a maximum of approximately 0.6 pH/h at 50 mM HEPES, indicating that the rate of pHi recovery in frog muscle is limited by the diffusion of buffer through an external "unstirred layer". In detubulated fibers, pHi recovery from acidification due to both the amiloride-sensitive Na+/H+ and the 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS)-sensitive (Na+ + HCO3-)/Cl- exchangers was nearly identical to recovery in fully tubulated fibers. This is consistent with these two pH recovery transporters being localized to the surface, and not the transverse tubular, membrane domain in frog skeletal muscle fibers.

scholarly journals Temperature dependence of the force‐generating process in single fibres from frog skeletal muscle

2003 ◽  
Vol 549 (1) ◽  
pp. 93-106 ◽  
Author(s):  
G. Piazzesi ◽  
M. Reconditi ◽  
N. Koubassova ◽  
V. Decostre ◽  
M. Linari ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Temperature Dependence ◽  
Frog Skeletal Muscle ◽  
Single Fibres
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