Characterization of Calcium-activated Chloride Channels in Patches Excised from the Dendritic Knob of Mammalian Olfactory Receptor Neurons

1998 ◽  
Vol 161 (2) ◽  
pp. 163-171 ◽  
Author(s):  
M. Hallani ◽  
J.W. Lynch †, ‡ , P.H. Barry
Keyword(s):  
Olfactory Receptor ◽  
Chloride Channels ◽  
Olfactory Receptor Neurons ◽  
Receptor Neurons

Functional properties of vertebrate olfactory receptor neurons

1986 ◽  
Vol 66 (3) ◽  
pp. 772-818 ◽  
Author(s):  
T. V. Getchell
Keyword(s):  
Olfactory Receptor ◽  
Olfactory Receptor Neuron ◽  
Olfactory Nerve ◽  
Sensory Transduction ◽  
Olfactory Receptor Neurons ◽  
Receptor Neuron ◽  
Olfactory Pathway ◽  
Receptor Neurons ◽  
Molecular Biological Techniques

The interaction of an odorant with the chemosensitive membrane of olfactory receptor neurons initiates a sequence of molecular and membrane events leading to sensory transduction, impulse initiation, and the transmission of sensory information to the brain. The main steps in this sequence are summarized in Figure 6. Several lines of evidence support the hypothesis that the initial molecular events and subsequent stages of transduction are mediated by odorant receptor sites and associated ion channels located in the membrane of the cilia and apical dendritic knob of the olfactory receptor neuron. Similarly, the membrane events associated with impulse initiation and propagation are mediated by voltage-gated channels located in the initial axonal segment and the axolemma. The ionic and electrical events associated with the proposed sequence have been characterized in general using a variety of experimental techniques. The identification, localization, and sequence of membrane events are consistent with the neurophysiological properties observed in specific regions of the bipolar receptor neuron. The influence of other cells in the primary olfactory pathway such as the sustentacular cells in the olfactory epithelium, the Schwann cells in the olfactory nerve, and the astrocytes in the olfactory nerve layer in the olfactory bulb on the physiological activity of the olfactory receptor neuron is an emerging area of research interests. The general principles derived from the experimental results described in this review provide only a framework that is both incomplete and of necessity somewhat speculative. As noted in the Introduction, the multidisciplinary study of the primary olfactory pathway is undergoing a renaissance of research interest. The application of modern biophysical, cell, and molecular biological techniques to the basic issues of odorant recognition and membrane excitability will clarify the speculations and lead to the establishment of new hypotheses. Three broad areas of research will benefit from such studies. First, the application of biophysical techniques will lead to a detailed characterization of the membrane properties and associated ion conductance mechanisms. Second, the isolation and biochemical characterization of intrinsic membrane and cytosolic proteins associated with odorant recognition, sensory transduction, and the subsequent electrical events will result from the utilization of cell and molecular biological techniques.(ABSTRACT TRUNCATED AT 400 WORDS)

Molecular and functional characterization of an Ih-channel from lobster olfactory receptor neurons

2005 ◽  
Vol 21 (6) ◽  
pp. 1635-1647 ◽  
Author(s):  
Günter Gisselmann ◽  
Thomas Marx ◽  
Yuriy Bobkov ◽  
Christian H. Wetzel ◽  
Eva M. Neuhaus ◽  
...  
Keyword(s):  
Olfactory Receptor ◽  
Functional Characterization ◽  
Olfactory Receptor Neurons ◽  
Ih Channel ◽  
Receptor Neurons

Characterization of a plasma membrane Ca2+ ATPase expressed in olfactory receptor neurons of the moth Spodoptera littoralis

2012 ◽  
Vol 350 (2) ◽  
pp. 239-250 ◽  
Author(s):  
Adrien François ◽  
Françoise Bozzolan ◽  
Elodie Demondion ◽  
Nicolas Montagné ◽  
Philippe Lucas ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Olfactory Receptor ◽  
Spodoptera Littoralis ◽  
Olfactory Receptor Neurons ◽  
Receptor Neurons

scholarly journals Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons

2018 ◽  
Author(s):  
Joseph D. Zak ◽  
Julien Grimaud ◽  
Rong-Chang Li ◽  
Chih-Chun Lin ◽  
Venkatesh N. Murthy
Keyword(s):  
Olfactory Receptor ◽  
Chloride Channels ◽  
Feedback Mechanism ◽  
Olfactory Receptor Neurons ◽  
Odor Source ◽  
Wild Type ◽  
Behavioral Deficits ◽  
Receptor Neurons

AbstractThe calcium-activated chloride channel anoctamin-2 (Ano2) is thought to amplify transduction currents in ORNs, a hypothesis supported by previous studies in dissociated neurons from Ano2-/- mice. Paradoxically, despite a reduction in transduction currents in Ano2-/- ORNs, their spike output for odor stimuli may be higher. We examined the role of Ano2 in ORNs in their native environment in freely breathing mice by imaging activity in ORN axons as they arrive in the olfactory bulb glomeruli. Odor-evoked responses in ORN axons of Ano2-/- mice were consistently larger for a variety of odorants and concentrations. In an open arena, Ano2-/- mice took longer to approach a localized odor source than wild-type mice, revealing clear olfactory behavioral deficits. Our studies provide the first in vivo evidence toward an alternative role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp ORN spike output.

scholarly journals Characterization of voltage- and Ca(2+)-activated K+ channels in squid olfactory receptor neurons.

1997 ◽  
Vol 200 (11) ◽  
pp. 1571-1586
Author(s):  
M T Lucero ◽  
N Chen
Keyword(s):  
Olfactory Receptor ◽  
Cell Voltage ◽  
Olfactory Receptor Neurons ◽  
Delayed Rectifier ◽  
Outward Currents ◽  
K Current ◽  
Receptor Neurons ◽  
Q10 Values ◽  
K Currents

We performed whole-cell voltage-clamp experiments on isolated olfactory neurons from the squid Lolliguncula brevis. Total outward currents were composed of three identifiable K+ currents: a delayed rectifier K+ current that showed slow inactivation and was sensitive to 5 mmol l-1 tetraethylammonium; a rapidly inactivating, 4-aminopyridine (4-AP)-sensitive, A-type K+ current and a Ca(2+)-sensitive K+ current that was blocked by 200 nmol l-1 charybdotoxin and 10 mmol l-1 Cd2+ but was insensitive to apamin. The proportion of each current type varied from cell to cell, suggesting that responses to a given odorant would depend of the complement of channels present. The kinetics of the K+ currents were affected by temperature, with Q10 values ranging from 2 to 6. The identification and characterization of these K+ currents will greatly aid our understanding of action potential generation in these cells and will facilitate modelling of how odor responses are transduced and coded in squid olfactory receptor neurons.

scholarly journals Characterization of Forskolin-Induced Ca2+ Signals in Rat Olfactory Receptor Neurons

2005 ◽  
Vol 97 (4) ◽  
pp. 510-518 ◽  
Author(s):  
Ken-ichi Otsuguro ◽  
Shree Hari Gautam ◽  
Shigeo Ito ◽  
Yoshiaki Habara ◽  
Toshiyuki Saito
Keyword(s):  
Olfactory Receptor ◽  
Olfactory Receptor Neurons ◽  
Receptor Neurons

scholarly journals Initial Characterization of a Subpopulation of Inherent Oscillatory Mammalian Olfactory Receptor Neurons

2019 ◽  
Vol 44 (8) ◽  
pp. 583-592 ◽  
Author(s):  
Kirill Ukhanov ◽  
Yuriy V Bobkov ◽  
Jeffrey R Martens ◽  
Barry W Ache
Keyword(s):  
Olfactory Receptor ◽  
Dynamic Properties ◽  
Rhythmic Activity ◽  
Olfactory Receptor Neurons ◽  
Odor Stimulus ◽  
Fundamental Feature ◽  
Published Evidence ◽  
Odor Plumes ◽  
Receptor Neurons

Abstract Published evidence suggests that inherent rhythmically active or “bursting” primary olfactory receptor neurons (bORNs) in crustaceans have the previously undescribed functional property of encoding olfactory information by having their rhythmicity entrained by the odor stimulus. In order to determine whether such bORN-based encoding is a fundamental feature of olfaction that extends beyond crustaceans, we patch-clamped bORN-like ORNs in mice, characterized their dynamic properties, and show they align with the dynamic properties of lobster bORNs. We then characterized bORN-like activity by imaging the olfactory epithelium of OMP-GCaMP6f mice. Next, we showed rhythmic activity is not dependent upon the endogenous OR by patching ORNs in OR/GFP mice. Lastly, we showed the properties of bORN-like ORNs characterized in mice generalize to rats. Our findings suggest encoding odor time should be viewed as a fundamental feature of olfaction with the potential to be used to navigate odor plumes in animals as diverse as crustaceans and mammals.

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