A Novel Approach to Study the Geometry of the Water Lumen of Ion Channels: Colicin Ia Channels in Planar Lipid Bilayers

1998 ◽  
Vol 161 (1) ◽  
pp. 83-92 ◽  
Author(s):  
O.V. Krasilnikov *, † , J.B. Da Cru ◽  
R.A. Nogueira
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Novel Approach ◽  
Colicin Ia

scholarly journals Microfabricated Teflon Membranes for Low-Noise Recordings of Ion Channels in Planar Lipid Bilayers

2003 ◽  
Vol 85 (4) ◽  
pp. 2684-2695 ◽  
Author(s):  
Michael Mayer ◽  
Jennah K. Kriebel ◽  
Magdalena T. Tosteson ◽  
George M. Whitesides
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Low Noise ◽  
Planar Lipid Bilayers

Formation of ion channels by Colicin B in planar lipid bilayers

1990 ◽  
Vol 114 (1) ◽  
pp. 79-95 ◽  
Author(s):  
J. O. Bullock ◽  
S. K. Armstrong ◽  
J. L. Shear ◽  
D. P. Lies ◽  
M. A. McIntosh
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Colicin B

Ion Channel Sensor on a Silicon Support

2004 ◽  
Vol 820 ◽  
Author(s):  
Michael Goryll ◽  
Seth Wilk ◽  
Gerard M. Laws ◽  
Stephen M. Goodnick ◽  
Trevor J. Thornton ◽  
...  
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Chemical Vapor ◽  
Fast Method ◽  
Fluorocarbon Films ◽  
Novel Approach ◽  
Voltage Dependent ◽  
Assembled Monolayers

AbstractWe are building a biosensor based on ion channels inserted into lipid bilayers that are suspended across an aperture in silicon. The process flow only involves conventional optical lithography and deep Si reactive ion etching to create micromachined apertures in a silicon wafer. In order to provide surface properties for lipid bilayer attachment that are similar to those of the fluorocarbon films that are currently used, we coated the silicon surface with a fluoropolymer using plasma-assisted chemical vapor deposition. When compared with the surface treatment methods using self-assembled monolayers of fluorocarbon chemicals, this novel approach towards modifying the wettability of a silicon dioxide surface provides an easy and fast method for subsequent lipid bilayer formation. Current-Voltage measurements on OmpF ion channels incorporated into these membranes show the voltage dependent gating action expected from a working porin ion channel.

scholarly journals Ion channels formed in planar lipid bilayers byBacillus thuringiensistoxins in the presence ofManduca sextamidgut receptors

FEBS Letters ◽  
1997 ◽  
Vol 412 (2) ◽  
pp. 270-276 ◽  
Author(s):  
Jean-Louis Schwartz ◽  
Yiang-Jiang Lu ◽  
Petra Söhnlein ◽  
Roland Brousseau ◽  
Raynald Laprade ◽  
...  
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers
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