Caco-2 Cell Line Used as an In Vitro Model to Study Cadmium Accumulation in Intestinal Epithelial Cells

1997 ◽  
Vol 158 (1) ◽  
pp. 31-48 ◽  
Author(s):  
C. Jumarie ◽  
P.G.C. Campbell ◽  
A. Berteloot ◽  
M. Houde ◽  
F. Denizeau
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
In Vitro Model ◽  
Intestinal Epithelial Cells ◽  
Cadmium Accumulation ◽  
Intestinal Epithelial ◽  
Vitro Model

scholarly journals Understanding mammary gland functioning in livestock species using in-vitro mammary epithelial cells model -An overview

2013 ◽  
Vol 3 (1) ◽  
pp. 160-168
Author(s):  
M Mukesh ◽  
Umesh Kumar Shandilya ◽  
Monika Sodhi ◽  
Ankita Sharma ◽  
Neha Kapila
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Cell Line ◽  
In Vitro Model ◽  
Mammary Epithelial Cells ◽  
Branching Morphogenesis ◽  
Mammary Epithelial ◽  
Livestock Species ◽  
Vitro Model

Understanding the mechanisms of the development of the mammary gland can increase the efficiency of milk production, as well as improve animal health. Mammary epithelial cells (MEC) are the functional unit of the mammary gland. Although, there is a well-established MEC cell line, known as MAC-T, the use of a primary cell line is preferred because it more closely mimics an in vivo model. This review focuses on utilization of MECs as a potential in vitro model to better understand mammary gland functions in livestock species. Recently, considerable advances in three dimensional modeling of the mammary gland have been made with the used of extracellular matrix for the study of branching morphogenesis which may enable rapid advances in our understanding of mammary gland biology. Progress in the exploitation of MECs as in vitro model is more productive than ever, however further research is vital.    

scholarly journals Isolation, culture, and characterization of chicken intestinal epithelial cells

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Federico Ghiselli ◽  
Barbara Rossi ◽  
Martina Felici ◽  
Maria Parigi ◽  
Giovanni Tosi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Protective Function ◽  
Novel Method ◽  
Vitro Model ◽  
Epithelial Cell Population

Abstract Background Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors and contribute to the development of strategies against pathogens. Results The present study describes a novel method to culture and maintain primary chicken enterocytes and their characterization by immunofluorescence and biomolecular approaches. Starting from 19-day-old chicken embryos it was possible to isolate viable intestinal cell aggregates that can expand and produce a self-maintaining intestinal epithelial cell population that survives until 12 days in culture. These cells resulted positive in immunofluorescence to Cytokeratin 18, Zonula occludens 1, Villin, and Occludin that are common intestinal epithelial markers, and negative to Vimentin that is expressed by endothelial cells. Cells were cultured also on Transwell® permeable supports and trans-epithelial electrical resistance, was measured. This value gradually increased reaching 64 Ω*cm2 7 days after seeding and it remained stable until day 12. Conclusions Based on these results it was confirmed that it is possible to isolate and maintain chicken intestinal epithelial cells in culture and that they can be suitable as in vitro intestinal model for further studies.

scholarly journals Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 328 ◽  
Author(s):  
Claudio Salaris ◽  
Melania Scarpa ◽  
Marina Elli ◽  
Alice Bertolini ◽  
Simone Guglielmetti ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Plaque Assay ◽  
Immune Response Gene ◽  
Intestinal Epithelial ◽  
Antiviral Immune Response ◽  
Qrt Pcr ◽  
Anti Inflammatory

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.

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