Effect of Dicyclohexylcarbodiimide (DCCD) on Transport Parameters in the Frog Cornea Epithelium

2000 ◽  
Vol 174 (2) ◽  
pp. 97-103 ◽  
Author(s):  
G. Carrasquer ◽  
M. Li ◽  
M.A. Dinno
1997 ◽  
Vol 75 (3) ◽  
pp. 199-204
Author(s):  
G. Carrasquer ◽  
S. Yang ◽  
M. Schwartz ◽  
M.A. Dinno

1985 ◽  
Vol 249 (2) ◽  
pp. F185-F191
Author(s):  
G. Carrasquer ◽  
S. Ahn ◽  
M. Schwartz ◽  
W. S. Rehm

The effect of changing the K concentration in the aqueous solution was studied in the frog cornea. In general, when the K concentration was increased from 4 to 20 or 79 mM, the transepithelial PD and resistance decreased. If K was decreased from 79 to 4, 20 to 4, or 4 to 0 mM, or any other combination, the PD and resistance increased. These are normal PD responses. If after a K-free period of more than 5-10 min the K was increased to 4 mM, the PD increased, an anomalous PD response. If K was increased from 0 to 20 mM, there was an initial PD increase (anomalous response) followed by a PD decrease (normal response). If K was increased from 0 to 79 mM the PD decreased, normal response only. The resistance decreased with every increase in K concentration. Anomalous responses were abolished in Na-free solutions and in the presence of both 10(-3) M ouabain and 10(-4) M vanadate in the aqueous solution. We interpret the results on the basis of two pathways, a simple K-conductive pathway and an electrogenic Na-K-ATPase pump pathway with more Na's than K's per cycle. The normal or anomalous PD responses to changes in aqueous K concentration depend on the relative resistance of the two pathways.


1996 ◽  
Vol 211 (2) ◽  
pp. 205-210 ◽  
Author(s):  
G. Carrasquer ◽  
S. Yang ◽  
M. Schwartz ◽  
M. A. Dinno

Author(s):  
Gaspar Carrasquer ◽  
Ming Li ◽  
Shen Yang ◽  
Manuel Schwartz ◽  
Mumtaz A. Dinno

1997 ◽  
Vol 75 (3) ◽  
pp. 199-204 ◽  
Author(s):  
G Carrasquer ◽  
S Yang ◽  
M Schwartz ◽  
M A Dinno

1991 ◽  
Vol 223 ◽  
Author(s):  
A. Vaseashta ◽  
L. C. Burton

ABSTRACTKinetics of persistent photoconductivity, photoquenching, and thermal and optical recovery observed in low energy Ar+ bombarded on (100) GaAs surfaces have been investigated. Rate and transport equations for these processes were derived and simulated employing transport parameters, trap locations and densities determined by deep level transient spectroscopy. Excellent correlation was obtained between the results of preliminary simulation and the experimentally observed values. The exponential decay of persistent photoconductivity response curve was determined to be due to metastable electron traps with longer lifetime and is consistent with an earlier proposed model.


2017 ◽  
Vol 68 (5) ◽  
pp. 903-907
Author(s):  
Ecaterina Anca Serban ◽  
Ioana Diaconu ◽  
Elena Ruse ◽  
Georgiana Ileana Badea ◽  
Adriana Cuciureanu ◽  
...  

Indole-3-acetic acid is a growth phytohormone considered the most important representative of auxin class. This paper presents the assessment of some kinetic parameters in the process of transport of indole-3-acetic acid taking into consideration the kinetic model of consecutive irreversible first order reactions. It was pursued the influence upon the process of parameters such as: feed phase concentration, stripping phase concentration in the presence of two type carriers: tributyl phosphate (TBP) and trioctylphosphine oxide (TOPO). Depending on these transport parameters were calculated kinetics parameters such as: pseudo-first-order apparent membrane entrance and exit rate constants, the maximum flux at the entrance and exit out of the membrane. The highest values of the transport flux is obtained in the presence of carrier trioctylphosphine oxide (TOPO) at the concentration in the feed phase of 10-4 mol/L indole-3-acetic acid and a concentration of 10--2mol/L NaOH in the stripping phase.


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