Mycosporine-like amino acids prevent UVB-induced abnormalities during early development of the green sea urchin Strongylocentrotus droebachiensis

2001 ◽  
Vol 138 (2) ◽  
pp. 267-280 ◽  
Author(s):  
N. L. Adams ◽  
J. M. Shick
Keyword(s):  
Amino Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Strongylocentrotus Droebachiensis ◽  
Green Sea Urchin

scholarly journals New microsatellite loci for the green sea urchin Strongylocentrotus droebachiensis using universal M13 labelled markers

2014 ◽  
Vol 7 (1) ◽  
pp. 699 ◽  
Author(s):  
Marc B Anglès d’Auriac ◽  
Anders Hobæk ◽  
Hartvig Christie ◽  
Hege Gundersen ◽  
Camilla Fagerli ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Microsatellite Loci ◽  
Strongylocentrotus Droebachiensis ◽  
Green Sea Urchin

Synthesis of nuclear and cytoplasmic proteins in the early development of fishes and echinoderms

Development ◽  
1971 ◽  
Vol 26 (3) ◽  
pp. 611-622
Author(s):  
Maya R. Krigsgaber ◽  
Alla A. Kostomarova ◽  
Tamara A. Terekhova ◽  
Tatiana A. Burakova
Keyword(s):  
Amino Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Nuclear Volume ◽  
Sea Urchin Embryos ◽  
Cytoplasmic Proteins ◽  
Specific Activities ◽  
Strongylocentrotus Nudus ◽  
Silver Grains ◽  
Misgurnus Fossilis

Synthesis of nuclear and cytoplasmic proteins was studied biochemically and autoradiographically in early loach (Misgurnus fossilis) and sea-urchin (Strongylocentrotus nudus) embryos. After incubation with [14C]amino acids for 5–120 min the ratio of the specific activities of nuclear, mitochondrial and 12000 g supernatant proteins was shown to be equal approximately to 6:1:2 in loach embryos and to 8:4:3 in sea-urchin embryos independently of the duration of labelling. After incubation with [3H]amino acids the number of silver grains per unit section was on the average 2·4 times higher for nuclei than it was for cytoplasm at mid-blastula and mid-gastrula stages. At the mid-gastrula the vegeto-animal gradient of protein synthesis was found. A higher level of the synthesis of nuclear proteins as compared with that of cytoplasmic proteins appears to be related to an increase in the nuclear volume and the nucleo-cytoplasmic ratio during the early development of the loach and sea-urchin embryos.

Evidence for Association of Vibrio Echinoideorum with Tissue Necrosis on Body Shell of the Green Sea Urchin Strongylocentrotus Droebachiensis

2021 ◽  
Author(s):  
Jonathan Hira ◽  
Klara Stensvåg
Keyword(s):  
Sea Urchin ◽  
Virulence Genes ◽  
Vibrio Vulnificus ◽  
Sea Urchins ◽  
Opportunistic Pathogen ◽  
Significant Degree ◽  
Strongylocentrotus Droebachiensis ◽  
Host Immune System ◽  
Progressive Development ◽  
Green Sea Urchin

Abstract “Sea urchin lesion syndrome” is known as sea urchins disease with the progressive development of necrotic epidermal tissue and loss of external organs, including appendages on the outer body surface. Recently, a novel strain, Vibrio echinoideorum has been isolated from the lesions of green sea urchin (Strongylocentrotus droebachiensis), an economically important mariculture species in Norway. V. echinoideorum has not been reported elsewhere in association of with green sea urchin lesion syndrome. Therefore, in this study, an immersion based bacterial challenge experiment was performed to expose sea urchins (wounded and non-wounded) to V. echinoideorum, thereby mimicking a nearly natural host-pathogen interaction under controlled conditions. This infection experiment demonstrated that only the injured sea urchins developed the lesion to a significant degree when exposed to V. echinoideorum. Pure cultures of the employed bacterial strain was recovered from the infected animals and its identity was confirmed by the MALDI-TOF MS spectra profiling. Additionally, the hemolytic phenotype of V. echinoideorum substantiated its virulence potential towards the host, and this was also supported by the cytolytic effect on red spherule cells of sea urchins. Furthermore, the genome sequence of V. echinoideorum was assumed to encode potential virulence genes and were subjected for in silico comparison with the established virulence factors of Vibrio vulnificus and Vibrio tasmaniensis. This comparative virulence profile provided novel insights about virulence genes and their putative functions related to chemotaxis, adherence, invasion, evasion of the host immune system, and damage of host tissue and cells. Thus, it supports the pathogenicity of V. echinoideorum. In conclusion, the interaction of V. echinoideorum with injured sea urchins appears to be essential for the development of lesion syndrome and therefore, revealing its potentiality as an opportunistic pathogen.

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