Successful Detection of Active Osteoclasts In Situ by Systemic Administration of an Osteoclast-Specific Monoclonal Antibody

1998 ◽  
Vol 63 (2) ◽  
pp. 148-153 ◽  
Author(s):  
T. Kukita ◽  
A. Kukita ◽  
L. Xu ◽  
H. Maeda ◽  
T. Iijima
Keyword(s):  
Monoclonal Antibody ◽  
Systemic Administration ◽  
Specific Monoclonal Antibody

scholarly journals In Situ Assay of Basic Fibroblast Growth Factor in Cultured Cells with a Specific Monoclonal Antibody.

1993 ◽  
Vol 18 (5) ◽  
pp. 333-343
Author(s):  
Masami Minemura ◽  
Yoshino Yoshitake ◽  
Kouichi Matsuzaki ◽  
Akiharu Watanabe ◽  
Katsuzo Nishikawa
Keyword(s):  
Monoclonal Antibody ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cultured Cells ◽  
Fibroblast Growth ◽  
Specific Monoclonal Antibody ◽  
In Situ Assay

scholarly journals Heat Shock in C. elegans Induces Downstream of Gene Transcription andAccumulation of Double-Stranded RNA

2018 ◽  
Author(s):  
Marko Melnick ◽  
Patrick Gonzales ◽  
Joseph Cabral ◽  
Mary A. Allen ◽  
Robin D. Dowell ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Caenorhabditis Elegans ◽  
Heat Shock ◽  
Gene Transcription ◽  
Specific Monoclonal Antibody ◽  
Rna Seq ◽  
Antisense Transcripts ◽  
Double Stranded Rna ◽  
C Elegans

AbstractWe observed that heat shock of Caenorhabditis elegans leads to the formation of nuclear double-stranded RNA (dsRNA) foci, detectable with a dsRNA-specific monoclonal antibody. These foci significantly overlap with nuclear HSF-1 granules. To investigate the molecular mechanism(s) underlying dsRNA foci formation, we used RNA-seq to globally characterize total RNA and immunoprecipitated dsRNA from control and heat shocked worms. We find antisense transcripts are generally increased after heat shock, and a subset of both sense and antisense transcripts enriched in the dsRNA pool by heat shock overlap with dsRNA transcripts enriched by deletion of tdp-1, which encodes the C. elegans ortholog of TDP-43. Interestingly, transcripts involved in translation are over-represented in the dsRNAs induced by either heat shock or deletion of tdp-1. Also enriched in the dsRNA transcripts are sequences downstream of annotated genes (DoGs), which we globally quantified with a new algorithm. To validate these observations, we used fluorescence in situ hybridization (FISH) to confirm both antisense and downstream of gene transcription for eif-3.B, one of the affected loci we identified.

Therapeutic Potential of SARS-CoV-2-Specific Monoclonal Antibody CR3022

2020 ◽  
Author(s):  
Caroline Atyeo ◽  
Matthew D. Slein ◽  
Stephanie Fischinger ◽  
John Burke ◽  
Alexandra Schӓfer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Therapeutic Potential ◽  
Specific Monoclonal Antibody

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

SIRPα-specific monoclonal antibody enables antibody-dependent phagocytosis of neuroblastoma cells

2021 ◽  
Author(s):  
Meriem Bahri ◽  
Sareetha Kailayangiri ◽  
Sarah Vermeulen ◽  
Natacha Galopin ◽  
Claudia Rossig ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Neuroblastoma Cells ◽  
Specific Monoclonal Antibody

scholarly journals A new reliable and highly specific monoclonal antibody to detect the C‐terminal region of Silencer of Cytokine Signaling 1 (SOCS1)

2021 ◽  
Author(s):  
Stephanie E. Weissinger ◽  
Malena Zahn ◽  
Ralf Marienfeld ◽  
Claudia Tessmer ◽  
Gerhard Moldenhauer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Terminal Region ◽  
Cytokine Signaling ◽  
Specific Monoclonal Antibody

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

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