Quality criteria for the isotope dilution method with HRGC/MS

1996 ◽  
Vol 354 (7-8) ◽  
pp. 818-822 ◽  
Author(s):  
B. Henkelmann ◽  
K. -W. Schramm ◽  
C. Klimm ◽  
A. Kettrup
Keyword(s):  
Isotope Dilution ◽  
Quality Criteria ◽  
Isotope Dilution Method ◽  
Dilution Method

ON THE VALUE OF SOME PARAMETERS OF ADRENOCORTICAL GLUCOCORTICOID FUNCTION

1963 ◽  
Vol 44 (4) ◽  
pp. 499-504 ◽  
Author(s):  
M. Van Der Straeten ◽  
A. Vermeulen ◽  
N. Orie ◽  
P. Regniers
Keyword(s):  
Urinary Excretion ◽  
Adrenal Cortex ◽  
Isotope Dilution ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
Steroid Excretion ◽  
Colorimetric Methods

ABSTRACT The authors studied the correlation between cortisol production, as measured by an isotope dilution method, and the urinary excretion of total and free Porter-Silber chromogens, as well as of 17-ketogenic steroids. Although a significant correlation exists between total Porter-Silber chromogens, 17-ketogenic steroid excretion and cortisol production, discrepancies are occasionally observed. Hence, different colorimetric methods should be used to assess the glucocorticoid activity of the adrenal cortex.

Secretion rate of dehydroepiandrosterone and dehydroepiandrosterone sulfate in benign essential hypertension as compared to normal subjects

1970 ◽  
Vol 48 (12) ◽  
pp. 1308-1313 ◽  
Author(s):  
A. Shao ◽  
W. Nowaczynski ◽  
O. Kuchel ◽  
J. Genest
Keyword(s):  
Essential Hypertension ◽  
Isotope Dilution ◽  
Normal Subjects ◽  
Dehydroepiandrosterone Sulfate ◽  
Secretion Rate ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
Hypertensive Patients ◽  
Double Isotope ◽  
Column Chromatographic

A study of the secretion rate of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) in normal subjects and patients with benign essential hypertension by a double isotope dilution method showed a fivefold increase in the secretion rate of dehydroepiandrosterone (from 9.8 mg/day ± 3.1 mg/day S.D. to 52.0 mg/day ± 15.5 mg/day S.D.), and a sixfold increase of dehydroepiandrosterone sulfate in hypertensive patients (from 10.7 mg/day ± 2.9 mg/day S.D. to 60.7 mg/day ± 18.6 mg/day S.D.). This study was carried out following the administration of 14C-labelled DHEA and 3H-labelled DHEA-S and involved an initial column chromatographic separation of urinary DHEA-glucuronide and sulfate.

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