Electrochemical methods for quantification and characterization of metallothioneins induced in Mytilus galloprovincialis

1996 ◽  
Vol 354 (5-6) ◽  
pp. 529-534
Author(s):  
B. Raspor ◽  
Jasenka Pavicic
Keyword(s):  
Mytilus Galloprovincialis ◽  
Electrochemical Methods

SEPARATION AND CHARACTERIZATION OF ANTHOCYANINS BY ANALYTICAL AND ELECTROCHEMICAL METHODS

2011 ◽  
Vol 10 (5) ◽  
pp. 697-701 ◽  
Author(s):  
Firuta Fitigau ◽  
Claudia Popescu ◽  
Adina-Elena Segneanu ◽  
Ionel Balcu ◽  
Raluca Martagiu ◽  
...  
Keyword(s):  
Electrochemical Methods

scholarly journals Characterization of the Domoic Acid Uptake Mechanism of the Mussel (Mytilus galloprovincialis) Digestive Gland

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 458
Author(s):  
Juan Blanco ◽  
Carmen Mariño ◽  
Helena Martín ◽  
Gonzalo Álvarez ◽  
Araceli E. Rossignoli
Keyword(s):  
Digestive Gland ◽  
Domoic Acid ◽  
Mytilus Galloprovincialis ◽  
Metabolic Inhibitor ◽  
Acid Uptake ◽  
Free Medium ◽  
Shellfish Poisoning ◽  
Uptake Mechanism ◽  
Amnesic Shellfish Poisoning

Cultures of the mussel Mytilus galloprovincialis are frequently affected by accumulation of the amnesic shellfish poisoning toxin domoic acid (DA). This species is characterized by a fast uptake and release of the toxin. In this work, the main characteristics of the uptake mechanism have been studied by incubation of digestive gland thin slices in media with different composition and DA concentration. DA uptake seems to follow Michaelis–Menten kinetics, with a very high estimated KM (1722 µg DA mL−1) and a Vmax of 71.9 µg DA g−1 h−1, which is similar to those found for other amino acids in invertebrates. Replacement of NaCl from the incubation media by Cl-choline (Na+-free medium) did not significantly reduce the uptake, but replacement by sorbitol (Na+-free and Cl−-depleted medium) did. A new experiment replacing all chlorides with their equivalent gluconates (Na+- and Cl−-free medium) showed an important reduction in the uptake that should be attributed to the absence of chloride, pointing to a Na+-independent, Cl− (or anion-) dependent transporter. In media with Na+ and Cl−, neither decreasing the pH nor adding cyanide (a metabolic inhibitor) had significant effect on DA uptake, suggesting that the transport mechanism is not H+- or ATP-dependent. In a chloride depleted medium, lowering pH or adding CN increased the uptake, suggesting that other anions could, at least partially, substitute chloride.

scholarly journals Characterization of a 20 kHz sonoreactor. Part II: analysis of chemical effects by classical and electrochemical methods

2005 ◽  
Vol 12 (1-2) ◽  
pp. 67-72 ◽  
Author(s):  
V. Sáez ◽  
A. Frı́as-Ferrer ◽  
J. Iniesta ◽  
J. González-Garcı́a ◽  
A. Aldaz ◽  
...  
Keyword(s):  
Electrochemical Methods ◽  
Chemical Effects

Isolation and partial characterization of cadmium-induced metallothionein-like proteins in Mytilus galloprovincialis

1991 ◽  
Vol 36 (1-4) ◽  
pp. 249-265 ◽  
Author(s):  
J. Pavičić ◽  
E. Balestreri ◽  
P. Lenzi ◽  
B. Raspor ◽  
M. Branica ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Partial Characterization
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