Supercritical fluid extraction combined with solid phase extraction as sample preparation technique for the analysis of β-blockers in serum and urine

1998 ◽  
Vol 360 (5) ◽  
pp. 618-621 ◽  
Author(s):  
G. Meissner ◽  
Kari Hartonen ◽  
M.-L. Riekkola
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Solid Phase ◽  
Phase Extraction ◽  
Preparation Technique ◽  
Sample Preparation Technique ◽  
Β Blockers ◽  
Serum And Urine

Comparison of Different Sample Preparation Techniques for Untargeted Metabolomics utilizing Q-TOF LC/MS and MetaboAnalyst 4.0

2020 ◽  
Vol 08 ◽  
Author(s):  
Ozan Kaplan ◽  
Engin Koçak ◽  
Mustafa Çelebier
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Preparation Technique ◽  
Sample Preparation Technique ◽  
Ms Analysis ◽  
Ionization Mode ◽  
Human Blood Samples ◽  
Preparation Techniques

Background: Profiling the whole metabolome with a single injection is not an easy process because the chemical and physical properties of metabolites are totally different within each other and the analytical methodologies and datamining procedures need lots of effort to make such an approach for real. This reality leads researchers to select an already applied methodology for metabolite profiling and analyze the samples through identical techniques. Objective: In this study, it was focused whether the sample preparation techniques on human blood samples prior to QTOF LC/MS analysis affect the number of detectable peaks and to analyze the matched metabolites of these peaks. The results were compared within each other. Method: Precipitation of proteins with methanol, ultrafiltration (Amicon® Ultra 3 kDa 0.5 mL Centrifugal Filters), liquidphase extraction (EXtrelut® NT 3 cartridges) and solid-phase extraction (Supelco HybridSPE®-Phospholipid Cartridge) were used for sample preparation on commercial pooled plasmas samples. C18 column (Agilent Zorbax 1.8 μM, 50 x 2.1 mm) was used as the chromatography column. Q-TOF LC/MS analysis was performed on positive ionization mode. XCMS and MetaboAnalyst 4.0 - MS Peaks to Pathways utility were used to evaluate the raw data. Results: Although the number of detected peaks through precipitation with methanol was the highest one (624 peaks), the detected peaks observed through ultrafiltration sample preparation technique matched with the highest number of metabolite peaks (151 metabolites). The number of the matched peaks with metabolites on liquid phase extraction (81 metabolites) was higher than the ones for solid phase extraction (29 metabolites). Conclusion: The results in this study may provide a novel perspective to analytical chemists working with clinicians to select their sample preparation technique prior to Q-TOF LC/MS based untargeted metabolomic approaches.

Miniaturized solid-phase extraction as a sample preparation technique for the determination of phthalates in water

2002 ◽  
Vol 373 (1-2) ◽  
pp. 81-86 ◽  
Author(s):  
Yoshihiro Saito ◽  
Yuji Nakao ◽  
Motohiro Imaizumi ◽  
Yoriko Morishima ◽  
Yoshiaki Kiso ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Preparation Technique ◽  
Sample Preparation Technique
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