An excellent electrochemical aptasensor for amyloid-β oligomers based on a triple-helix aptamer switch via target-triggered signal transduction DNA displacement events

2021 ◽  
Author(s):  
Xiaoying Wang ◽  
Xuan Gu ◽  
Linyu Li ◽  
Bingjia Yu ◽  
Liangrui Lv ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Triple Helix ◽  
Amyloid Β ◽  
Electrochemical Aptasensor

A sensitive and versatile “signal-on” electrochemical aptasensor based on a triple-helix molecular switch

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6272-6278 ◽  
Author(s):  
Xiuzhong Wang ◽  
Aiwen Jiang ◽  
Ting Hou ◽  
Feng Li
Keyword(s):  
Triple Helix ◽  
Molecular Switch ◽  
Electrochemical Aptasensor

O3-04-03: Identification of a signal transduction mechanism of amyloid-β/PrPC interaction

2012 ◽  
Vol 8 (4S_Part_12) ◽  
pp. P435-P435
Author(s):  
Wataru Kudo ◽  
Hyun-pil Lee ◽  
Robert Petersen ◽  
Hyoung-gon Lee
Keyword(s):  
Signal Transduction ◽  
Amyloid Β ◽  
Transduction Mechanism ◽  
Signal Transduction Mechanism

A triple-helix molecular switch-based electrochemical aptasensor for interferon-gamma using a gold electrode and Methylene Blue as a redox probe

2017 ◽  
Vol 184 (10) ◽  
pp. 4151-4157 ◽  
Author(s):  
Khalil Abnous ◽  
Noor Mohammad Danesh ◽  
Mohammad Ramezani ◽  
Mona Alibolandi ◽  
Koroush Yousefi Hassanabad ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Interferon Gamma ◽  
Triple Helix ◽  
Gold Electrode ◽  
Molecular Switch ◽  
Electrochemical Aptasensor ◽  
Redox Probe

An electrochemical aptasensor for amyloid-β oligomer based on double-stranded DNA as “conductive spring”

2020 ◽  
Vol 187 (4) ◽  
Author(s):  
Chunyan Deng ◽  
Hui Liu ◽  
Shihui Si ◽  
Xiaojun Zhu ◽  
Qiuyun Tu ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Electrochemical Aptasensor ◽  
Double Stranded Dna

A signal on aptamer-based electrochemical sensing platform using a triple-helix molecular switch

2014 ◽  
Vol 6 (16) ◽  
pp. 6294-6300 ◽  
Author(s):  
Yudan Du ◽  
Yinfei Mao ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Genping Yan ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Triple Helix ◽  
Molecular Switch ◽  
Electrochemical Sensing ◽  
Label Free ◽  
Sensing Platform ◽  
Aptamer Sequence

A signal on aptamer-based electrochemical sensing platform was demonstrated by taking advantage of a triple-helix molecular switch, which contained a label-free target specific aptamer sequence and a hairpin-shaped structured signal transduction probe.

Abstract 482: Amyloid-β Peptides Induce a Signal Transduction via Activation of the α1-Adrenoreceptor in Vsmc and Cardiomyocytes

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Nadine Haase ◽  
Hannelore Haase ◽  
Florian Herse ◽  
Friedrich C Luft ◽  
Dominik N Muller ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Alzheimer Disease ◽  
Amyloid Β ◽  
Cardiac Contraction ◽  
Amyloid Angiopathy ◽  
Chronotropic Effect ◽  
Aβ Peptides ◽  
Chronotropic Response ◽  
Increased Risk ◽  
Receptor Blockers

Hypertension is associated with increased risk of Alzheimer disease (AD) that features Amyloid-β peptide (Aβ) deposition in brain (neuritic plaques) and in blood vessels (cerebral amyloid angiopathy). Recently, it was shown by others that Aβ peptides exert a chronotropic effect in neonatal cardiomyocytes, similar to α1-adrenoreceptor autoantibodies (α-AB) as we described earlier. We hypothesized that Aβ peptides elicit a signal transduction pathway in vascular cells, induced by activation of the α1-adrenoreceptor. Aβ peptides (10-35) and (25-35) induced a positive chorontropic effect in the cardiac contraction assay (CCA) after long term activation up to 4 hours (28.84±1.04 bpm and 28.96±1.08 bpm). The effect was attenuated by the α1-receptor blockers urapidil and prazosin (1.72±0.4 bpm and -0.76±1.16 bpm). β1, β2, AT1, or AT2 receptor blockers had no effect on the positive chronotropic effect of Aβ (10-35) and (25-35). Using confocal microscopy and phosphospecific immunoblotting we observed that both peptides induced ERK 1/2 phosphorylation in Chinese hamster ovary (CHO) cells, overexpressing the α1A adrenoreceptor (CHO-α1-AR), but not in wildtype CHO. pERK was also induced in vascular smooth muscle cells (VSMC) and cardiomyocytes. Both Aβ peptides induced an intracellular Ca2+ signal in VSMC. The positive chronotropic response to Aβ (25-35) in the CCA was blocked with a peptide corresponding to the 1st extracellular loop of the α1-AR, the identical epitope to which α1-AB binds. Our data show that Amyloid-β peptides induce a signal transduction cascade via the α1-adrenoreceptor in VSMC and cardiomyocytes. These data might help explain the newly discovered association between hypertension and Alzheimer disease. The role of Aβ peptides in vascular medicine deserves further study.

Triple-helix molecular switch-induced hybridization chain reaction amplification for developing a universal and sensitive electrochemical aptasensor

RSC Advances ◽  
2016 ◽  
Vol 6 (93) ◽  
pp. 90310-90317 ◽  
Author(s):  
Qiaoqiao Liu ◽  
Jinquan Liu ◽  
Dinggeng He ◽  
Taiping Qing ◽  
Xiaoxiao He ◽  
...  
Keyword(s):  
Triple Helix ◽  
Molecular Switch ◽  
Hybridization Chain Reaction ◽  
Electrochemical Aptasensor ◽  
Chain Reaction ◽  
Reaction Amplification

In this work, a universal and sensitive “signal-on” electrochemical aptasensor platform has been developed based on a triple-helix molecular switch (THMS)-induced hybridization chain reaction (HCR) amplification.

Signal transduction during amyloid-β-peptide neurotoxicity: role in Alzheimer disease

2004 ◽  
Vol 47 (1-3) ◽  
pp. 275-289 ◽  
Author(s):  
Rodrigo A. Fuentealba ◽  
Ginny Farias ◽  
Jessica Scheu ◽  
Miguel Bronfman ◽  
María Paz Marzolo ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Alzheimer Disease ◽  
Amyloid Β ◽  
Amyloid Β Peptide
Sign in / Sign up

Export Citation Format

Share Document