Disrupted carbonic anhydrase distribution in the avian shell gland following in ovo exposure to estrogen

2001 ◽  
Vol 75 (6) ◽  
pp. 362-368 ◽  
Author(s):  
Lena Holm ◽  
Cecilia Berg ◽  
Björn Brunström ◽  
Yvonne Ridderstråle ◽  
Ingvar Brandt
Keyword(s):  
Carbonic Anhydrase ◽  
Shell Gland ◽  
In Ovo

scholarly journals Embryonic exposure to oestrogen causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen

Reproduction ◽  
2004 ◽  
Vol 128 (4) ◽  
pp. 455-461 ◽  
Author(s):  
C Berg ◽  
A Blomqvist ◽  
L Holm ◽  
I Brandt ◽  
B Brunström ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Environmental Concern ◽  
Carbonic Anhydrase Activity ◽  
Shell Gland ◽  
In Ovo ◽  
Functional Disturbance ◽  
Embryonic Exposure ◽  
Eggshell Thinning ◽  
Resistance To Deformation ◽  
Domestic Hen

Eggshell thinning among wild birds has been an environmental concern for almost half a century. Although the mechanisms for contaminant-induced eggshell thinning are not fully understood, it is generally conceived to originate from exposure of the laying adult female. Here we show that eggshell thinning in the domestic hen is induced by embryonic exposure to the synthetic oestrogen ethynyloestradiol. Previously we reported that exposure of quail embryos to ethynyloestradiol caused histological changes and disrupted localization of carbonic anhydrase in the shell gland in the adult birds, implying a functional disturbance in the shell gland. The objective of this study was to examine whether in ovo exposure to ethynyloestradiol can affect eggshell formation and quality in the domestic hen. When examined at 32 weeks of age, hens exposed to ethynyloestradiol in ovo (20 ng/g egg) produced eggs with thinner eggshells and reduced strength (measured as resistance to deformation) compared with the controls. These changes remained 14 weeks later, confirming a persistent lesion. Ethynyloestradiol also caused a decrease in the number of shell gland capillaries and in the frequency of shell gland capillaries with carbonic anhydrase activity. These data suggested that a disrupted carbonic anhydrase expression was involved in the mechanism for the oestrogen-induced eggshell thinning found in this study. The results support our hypothesis that eggshell thinning in avian wildlife can result from a structural and functional malformation in the shell gland, induced by xeno-oestrogen exposure during embryonic development.

scholarly journals Shell Strength and Carbonic Anhydrase Activity of the Shell Gland of the Domestic Fowl

1968 ◽  
Vol 47 (3) ◽  
pp. 858-862 ◽  
Author(s):  
P.J. Heald ◽  
Dianne Pohlman ◽  
E.G. Martin
Keyword(s):  
Carbonic Anhydrase ◽  
Domestic Fowl ◽  
Carbonic Anhydrase Activity ◽  
Shell Gland

scholarly journals Functional involvement of carbonic anhydrase in calcium transport of the chick chorioallantoic membrane

1978 ◽  
Vol 176 (1) ◽  
pp. 67-74 ◽  
Author(s):  
R S Tuan ◽  
J Zrike
Keyword(s):  
Carbonic Anhydrase ◽  
Chorioallantoic Membrane ◽  
Carbonic Anhydrase Activity ◽  
Calcium Deposition ◽  
Dependent Manner ◽  
Transport Activity ◽  
In Ovo ◽  
Chick Chorioallantoic Membrane ◽  
Age Dependent

Carbonic anhydrase activity was demonstrated in the chick-embryonic chorioallantoic membrane and was correlated with the Ca2+-transport activity of the membrane. It is inhibited by sulphonamides and is expressed in the chorioallantoic membrane in an age-dependent fashion during embryonic development. Ca2+ uptake by the chorioallantoic membrane in vivo also increases in a similar age-dependent manner. The temporal increase in these activities is coincident with calcium deposition in the embryonic skeleton. Incubation of the chorioallantoic membrane in ovo with sulphonamides specifically inhibits both the carbonic anhydrase and the Ca2+ uptake activities of the membrane in vivo. Enzyme histochemistry revealed the carbonic anhydrase activity is localized in the Ca2+-transporting ectodermal cells of the chorioallantoic membrane. These results, taken together, indicate that carbonic anhydrase may be functionally important in the Ca2+-transport activity of the chorioallantoic membrane.

scholarly journals LOCALIZATION OF CARBONIC ANHYDRASE IN AVIAN GASTRIC MUCOSA, SHELL GLAND AND BONE BY IMMUNOHISTOCHEMISTRY

1974 ◽  
Vol 22 (8) ◽  
pp. 819-825 ◽  
Author(s):  
CAROL V. GAY ◽  
HARALD SCHRAER ◽  
EDWARD J. FALESKI ◽  
ROSEMARY SCHRAER
Keyword(s):  
Carbonic Anhydrase ◽  
Fluorescent Antibody ◽  
Fluorescein Isothiocyanate ◽  
Shell Gland ◽  
Significant Finding ◽  
Specific Fluorescence ◽  
Tubular Gland ◽  
Γ Globulins ◽  
Mucosal Lining ◽  
Avian Erythrocyte

Antisera to purified avian erythrocyte carbonic anhydrase (CA) were produced in rabbits and the γ-globulin fractions were isolated and purified by ammonium sulfate precipitation and diethylaminoethyl-Sephadex chromatography. The anti-CA immuno-γ-globulins were tested by immunoelectrophoresis and judged to be highly specific for CA. Fluorescein isothiocyanate-conjugated goat antirabbit γ-globulins were used in the indirect fluorescent antibody localization of CA in cryostat sections. Specific fluorescence of CA was observed in gastric mucosal lining cells, shell gland columnar and tubular gland cells, red blood cells, erythroblasts and osteoclasts. Specific fluorescence was absent in the several immunologic controls, including the blocking test. Specific fluorescence was also lacking in tissue constituents which contain no detectable amounts of CA, i.e., muscle, connective tissue, blood vessels and nuclei. A significant finding in this study is the occurrence of cross-reactions between antibodies to red blood cell CA and CA from other tissues, indicating the immunologic similarity of CA from different tissue sources.

EMBRYONIC EXPOSURE TO O,P′-DDT CAUSES EGGSHELL THINNING AND ALTERED SHELL GLAND CARBONIC ANHYDRASE EXPRESSION IN THE DOMESTIC HEN

2006 ◽  
Vol 25 (10) ◽  
pp. 2787 ◽  
Author(s):  
Lena Holm ◽  
Alexandra Blomqvist ◽  
Ingvar Brandt ◽  
Björn Brunström ◽  
Yvonne Ridderstråle ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Shell Gland ◽  
Embryonic Exposure ◽  
Eggshell Thinning ◽  
Domestic Hen

The influence of saline drinking water on the activity of carbonic anhydrase in the shell gland of laying hens

1989 ◽  
Vol 40 (5) ◽  
pp. 1111 ◽  
Author(s):  
I Yoselewitz ◽  
D Balnave
Keyword(s):  
Drinking Water ◽  
Carbonic Anhydrase ◽  
Laying Hens ◽  
Specific Activity ◽  
Carbonic Anhydrase Activity ◽  
Shell Gland ◽  
Shell Quality ◽  
The Poor ◽  
Egg Shell ◽  
Reduced Activity

Carbonic anhydrase activity was measured in three experiments using mucosal extracts from the shell gland of laying hens given drinking water consisting of town water with or without the addition of sodium chloride (2 g/l). Hens laying eggs with normal or defective shells on both treatments were used. The specific activity of carbonic anhydrase was significantly lower in hens receiving the saline drinking water, but egg shell type had no significant effect on enzyme activity. The results indicate that a reduced activity of carbonic anhydrase in the shell gland contributes to the poor egg shell quality observed in hens receiving saline drinking water.

Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken

1992 ◽  
Vol 262 (5) ◽  
pp. R800-R805 ◽  
Author(s):  
A. Bar ◽  
S. Striem ◽  
E. Vax ◽  
H. Talpaz ◽  
S. Hurwitz
Keyword(s):  
Carbonic Anhydrase ◽  
Sexual Maturation ◽  
Carbonic Anhydrase Inhibitor ◽  
Temporal Association ◽  
Shell Gland ◽  
Half Time ◽  
Synthetic Oligonucleotide ◽  
Egg Shell

A synthetic oligonucleotide was used as a probe for measurement of calbindin mRNA in the shell gland and intestine of chickens. The half time of calbindin mRNA in the duodenum and shell gland was estimated at 2 and 3.6 h and that of calbindin at 13.9 and 32.6 h, respectively. The formation rates of calbindin mRNA were 0.37 and 0.17 pmol.h-1.g-1 and the rate of calbindin formation was 0.099 and 0.031 microgram.pmol mRNA-1.h-1 in the duodenum and shell gland, respectively. In the shell gland, calbindin mRNA and calbindin appeared at the time of sexual maturation during calcification of the first egg shell. Calbindin mRNA fluctuated markedly during the daily egg cycle, in close temporal association with egg shell calcification. When Ca2+ deposition was eliminated by expulsion of the ovum, the rise in calbindin mRNA was prevented. An indirect suppression of Ca2+ deposition by administration of the carbonic anhydrase inhibitor acetazolamide also resulted in a decrease in calbindin mRNA. The results are consistent with a possible role of Ca2+ flux in the regulation of calbindin mRNA appearance in the shell gland of chickens.

Sign in / Sign up

Export Citation Format

Share Document