Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis

2000 ◽  
Vol 74 (10) ◽  
pp. 602-608 ◽  
Author(s):  
Zhi-Zhong Guan ◽  
Kai-Qi Xiao ◽  
Xian-Yun Zeng ◽  
Yi-Guo Long ◽  
Yuan-Hua Cheng ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Composition ◽  
Cellular Membrane ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Chronic Fluorosis

Impact of PrsA on membrane lipid composition during daptomycin-resistance-mediated β-lactam sensitization in clinical MRSA strains

2021 ◽  
Author(s):  
Carla C C R de Carvalho ◽  
Agustina Taglialegna ◽  
Adriana E Rosato
Keyword(s):  
Lipid Composition ◽  
Cellular Membrane ◽  
Membrane Lipid ◽  
Antibiotic Concentration ◽  
Membrane Lipid Composition ◽  
Oxacillin Resistance ◽  
Lactam Antibiotic ◽  
Severe Infections ◽  
Resistant Strains ◽  
Mrsa Strains

Abstract Background The cyclic anionic lipopeptide daptomycin is used in the treatment of severe infections caused by Gram-positive pathogens, including MRSA. Daptomycin resistance, although rare, often results in treatment failure. Paradoxically, in MRSA, daptomycin resistance is usually accompanied by a concomitant decrease in β-lactam resistance in what is known as the ‘see-saw effect’. This resensitization is extensively used for the treatment of MRSA infections, by combining daptomycin and a β-lactam antibiotic, such as oxacillin. Objectives We aimed: (i) to investigate the combined effects of daptomycin and oxacillin on the lipid composition of the cellular membrane of both daptomycin-resistant and -susceptible MRSA strains; and (ii) to assess the involvement of the post-translocational protein PrsA, which plays an important role in oxacillin resistance in MRSA, in membrane lipid composition and remodelling during daptomycin resistance/β-lactam sensitization. Results The combination of microbiological and biochemical studies, with fluorescence microscopy using lipid probes, showed that the lipid composition and surface charge of the daptomycin-resistant cells exposed to daptomycin/oxacillin were dependent on antibiotic concentration and directly associated with PrsA, which influenced cardiolipin remodelling/relocation. Conclusions Our findings show that PrsA, in addition to its post-transcriptional role in the maturation of PBP 2a, is a key mediator of cell membrane remodelling connected to the see-saw effect and may have a key role in the resensitization of daptomycin-resistant strains to β-lactams, such as oxacillin.

scholarly journals P4-199: Role of γ secretase in the cellular membrane lipid composition

2008 ◽  
Vol 4 ◽  
pp. T729-T730
Author(s):  
Jessica Hommes ◽  
Jochen Walter ◽  
Bernadette Breiden ◽  
Irfan Y. Tamboli ◽  
Günter Schwarzmann
Keyword(s):  
Lipid Composition ◽  
Cellular Membrane ◽  
Membrane Lipid ◽  
Membrane Lipid Composition
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