Molecular characterization and heterologous expression of hypCD , the first two [NiFe] hydrogenase accessory genes of Thermococcus litoralis

2001 ◽  
Vol 176 (3) ◽  
pp. 231-235 ◽  
Author(s):  
Mária Takács ◽  
Gábor Rákhely ◽  
Kornél Kovács
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Thermococcus Litoralis ◽  
Accessory Genes

Molecular characterization and heterologous expression of an endo-β-1,6-glucanase gene from the mycoparasitic fungus Trichoderma harzianum

1995 ◽  
Vol 247 (5) ◽  
pp. 639-645 ◽  
Author(s):  
José M. Lora ◽  
Jesús De la Cruz ◽  
Antonio Llobell ◽  
Tahía Benítez ◽  
José A. Pintor-Toro
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Trichoderma Harzianum ◽  
Mycoparasitic Fungus

Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

2010 ◽  
Vol 75 (4) ◽  
pp. 452-459 ◽  
Author(s):  
A. S. Vangnai ◽  
W. Promden ◽  
W. De-Eknamkul ◽  
K. Matsushita ◽  
H. Toyama
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Gluconobacter Oxydans ◽  
Dehydrogenase Gene

Heterologous Expression of SARS-CoV ORF10 and X5 Genes in E. coli and Streptomyces lividans TK24

2007 ◽  
Vol 62 (9-10) ◽  
pp. 765-771
Author(s):  
Jiang-qiang Kong ◽  
Wei Wang ◽  
Guan-hua Du ◽  
Ping Zhu ◽  
Ke-di Cheng
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Western Blot ◽  
Heterologous Expression ◽  
Streptomyces Lividans ◽  
Potential Functions ◽  
Structural Genes ◽  
First Report ◽  
E Coli ◽  
Accessory Genes ◽  
Sds Page

In previous studies a variety of novel accessory genes has been identified that were interspersed among the structural genes of the SARS-CoV (severe acute respiratory syndrome coronavirus) genome. The predicted unknown proteins (PUPs) encoded by the accessory genes, which are considered to be unique to the SARS-CoV genome, might play important roles in the SARS-CoV infection. Two of these genes, called ORF10 and X5, were synthesized and introduced into E. coli and Streptomyces lividans TK24, respectively. SDS-PAGE and Western blot revealed that the ORF10 and X5 genes have been expressed in the two hosts. This is the first report of heterologous expression of ORF10 and X5 genes in E. coli and S. lividans TK24. This work makes it possible to study the structure and potential functions of proteins encoding by these two genes.

Heterologous expression and molecular characterization of the NAD(P)H:acceptor oxidoreductase (FerB) of Paracoccus denitrificans

2009 ◽  
Vol 68 (2) ◽  
pp. 233-238 ◽  
Author(s):  
Radek Tesařík ◽  
Vojtěch Sedláček ◽  
Jana Plocková ◽  
Michaela Wimmerová ◽  
Jaroslav Turánek ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Paracoccus Denitrificans

scholarly journals Cloning, molecular characterization and heterologous expression of AMY1, an α-amylase gene from Cryptococcus flavus

2008 ◽  
Vol 280 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Alexsandro S. Galdino ◽  
Cirano J. Ulhoa ◽  
Lídia Maria P. Moraes ◽  
Maura V. Prates ◽  
Carlos Bloch ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Amylase Gene
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