Reversible hydrogenase in

1994 ◽  
Vol 161 (2) ◽  
pp. 140 ◽  
Author(s):  
Larissa Serebriakova ◽  
Nikolaj A. Zorin ◽  
Peter Lindblad
Keyword(s):  
Reversible Hydrogenase

Electrochemical Study of Reversible Hydrogenase Reaction ofDesulfovibrio vulgarisCells with Methyl Viologen as an Electron Carrier

1999 ◽  
Vol 71 (9) ◽  
pp. 1753-1759 ◽  
Author(s):  
Hirosuke Tatsumi ◽  
Kazuyoshi Takagi ◽  
Megumi Fujita ◽  
Kenji Kano ◽  
Tokuji Ikeda
Keyword(s):  
Methyl Viologen ◽  
Electrochemical Study ◽  
Electron Carrier ◽  
Reversible Hydrogenase

Reversible hydrogenase in Anabaena variabilis ATCC 29413

1994 ◽  
Vol 161 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Larissa Serebriakova ◽  
Nikolaj A. Zorin ◽  
Peter Lindblad
Keyword(s):  
Anabaena Variabilis ◽  
Reversible Hydrogenase

The reversible hydrogenase inAnacystis nidulans is a component of the cytoplasmic membrane

1991 ◽  
Vol 78 (12) ◽  
pp. 559-560 ◽  
Author(s):  
T. Kentemich ◽  
M. Casper ◽  
H. Bothe
Keyword(s):  
Cytoplasmic Membrane ◽  
Reversible Hydrogenase

Localization of the Reversible Hydrogenase in Cyanobacteria

1989 ◽  
Vol 44 (5-6) ◽  
pp. 384-391 ◽  
Author(s):  
Thomas Kentemich ◽  
Margret Bahnweg ◽  
Frank Mayer ◽  
Hermann Bothe
Keyword(s):  
Western Blot ◽  
Blot Analysis ◽  
Cytoplasmic Membrane ◽  
Specific Activity ◽  
Protein A ◽  
Anacystis Nidulans ◽  
Cell Periphery ◽  
Native Page ◽  
Activity Staining ◽  
Reversible Hydrogenase

Abstract The reversible hydrogenase of the cyanobacterium Anacystis nidulans was purified 250-fold by classical methods. Activity staining on gels obtained by native PAGE allowed to identify two bands. A ntibodies were raised against the electrophoretically homogeneous protein. The molecu­lar weight of the hydrogenase was determined by SD S-PAGE follow ed by Western blot analysis with these antibodies. Crude extracts from the unicellular Anacystis and from heterocysts and vegetative cells of Anabaena variabilis showed precipitation bands of 56 and 17 kd. The reversible hydrogenase appeared to be com posed o f a larger and a smaller subunit as the NiFeS-hydrogen-ases from other bacteria. The hydrogenase subunits, particularly the smaller on e, were subject to proteolysis. By application of the protein A-immuno-gold labeling technique the reversible hydro­ genase was shown to associate with the cytoplasmic membrane in Anacystis. When A nacystis was grown microaerobically, the increase in the specific activity of the reversible hydrogenase paral­leled the raise in the gold-labeling at the cell periphery as measured from the electron micro­ graphs. It is concluded that Anacystis has two different hydrogenases: the reversible or bidirec­tional hydrogenase which is located exclusively at the cytoplasmic membrane of the cells, and a thylakoid-bound enzyme which catalyzes only the uptake of H2.

scholarly journals Reversible hydrogenase activity of Gloeocapsa alpicola in continuous culture

1998 ◽  
Vol 166 (1) ◽  
pp. 89-94 ◽  
Author(s):  
L.T Serebryakova ◽  
M Sheremetieva ◽  
A.A Tsygankov
Keyword(s):  
Continuous Culture ◽  
Hydrogenase Activity ◽  
Reversible Hydrogenase

scholarly journals Reversible hydrogenase of Anabaena variabilis ATCC 29413: catalytic properties and characterization of redox centres

FEBS Letters ◽  
1996 ◽  
Vol 383 (1-2) ◽  
pp. 79-82 ◽  
Author(s):  
Larissa T. Serebryakova ◽  
Milagros Medina ◽  
Nikolay A. Zorin ◽  
Ivan N. Gogotov ◽  
Richard Cammack
Keyword(s):  
Catalytic Properties ◽  
Anabaena Variabilis ◽  
Properties And Characterization ◽  
Reversible Hydrogenase

scholarly journals Physiological Reactions of the Reversible Hydrogenase from Anabaena 7120

1981 ◽  
Vol 68 (3) ◽  
pp. 717-721 ◽  
Author(s):  
Jeffrey P. Houchins ◽  
Robert H. Burris
Keyword(s):  
Anabaena 7120 ◽  
Reversible Hydrogenase ◽  
Physiological Reactions

scholarly journals Accumulation of Ferrous Iron in Chlamydomonas reinhardtii. Influence of CO2 and Anaerobic Induction of the Reversible Hydrogenase

2003 ◽  
Vol 131 (4) ◽  
pp. 1756-1764 ◽  
Author(s):  
Boris K. Semin ◽  
Lira N. Davletshina ◽  
Alla A. Novakova ◽  
Tat'yana Y. Kiseleva ◽  
Victoriya Y. Lanchinskaya ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Ferrous Iron ◽  
Anaerobic Induction ◽  
Reversible Hydrogenase
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